Shared Flashcard Set

Details

zPRINCIPLES OF INFLAMMATION
Biochemical and Molecular Principles of Drug Action Biochemical and Molecular Principles of Drug Action
72
Pharmacology
Professional
03/16/2012

Additional Pharmacology Flashcards

 


 

Cards

Term
Innate responses
Definition
recognizing the non-self react to this stimulus: histamine release, phagocytosis, process the agent into small fragments, hence activate adaptive immune system.
Term
Adaptive responses
Definition
neutralizing/kill reactions that are specific to the offending agent: cytotoxic T cells, antibodies
Term
Three tasks of innate immunity
Definition
Defend against bacterial/parasitic infections via neutralization of the pathogen with secreted cytotoxic proteins or by phagocytosis of the bacteria/parasite.
Phagocytosis initiate proteolytic digestion of the macromolecules to fragments (antigen) that are then presented together with MHC II proteins on the surface of the antigen presenting cells (APC) which activates the adaptive immune system
APC= macrophages and dendrites
3) The innate immune cells secrete a variety of cytokines that further amplify the response.
Term
toll –like receptors
Definition
Transmembrane protein that bind to shared microbial component such as TLR4-LPS complex
TLR-ligand activates a cascade of events including expresion of pro-inflammatory cytokines, leading to further immune cell recruitment and activation of the inflammatory response
Term
IL-1
Definition
Enhances activity of NK cells
Attracts neutrophils and macrophages
Term
IL-2
Definition
Induces proliferation of antigen-primed T cells
Enhances activity of NK cells
Term
IFN-y
Definition
Enhances activity of macrophages and NK cells
Increases expression of MHC molecules
Enhances production of IgG2a
Term
TNF-a
Definition
Cytotoxic effects on tumor cells
Induces cytokines secretion in the inflammatory response
Term
An additional degree of specificit with MHC II APCs
Definition
provided by the interaction of CD28 on TH cells with proteins of the B7 family on APC; this “costimulatory signal” is required for TH activation. The activated TH cell secretes IL-2 and expresses the IL-2 receptor (IL-2R); this autocrine pathway stimulates further TH-cell proliferation and activation. IL-2 and other cytokines secreted by the TH cell activate not only TH cells, but also TC cells and B cells.
Term
Costimulation in the T-Cell Activation Pathway
Definition
Co- stimulation ensures that the stimulation of a single receptor does not elicits
a damage immune reaction: signal 1->provides specificity, signal 2->permissive
Term
Costimulation and the CD40–CD40L Interaction
Definition
A. An antigen-presenting cell (APC) presents MHC class II-bound antigen to a CD4+ T cell. T-cell recognition of antigen initiates an intracellular signaling cascade that leads to expression of CD40 ligand (CD40L) at the T-cell surface. B. CD40L on the activated T cell binds to CD40 on the surface of the APC. Activation of CD40 generates an intracellular signaling cascade that leads to expression of B7 on the APC surface. C. Enhanced T-cell proliferation and differentiation are promoted by costimulation of the T cell by MHC class II antigen (which binds to the T-cell receptor), CD40 (which binds to T-cell CD40L), and B7 (which binds to T-cell CD28). Cytokines secreted by the activated APC augment T-cell proliferation and differentiation.
Term
Histamine-
Definition
Stored in the granules of mast cells/basophiles. Vasodilatation of arterioles and constriction of veins, constriction of endothelial cells: vascular permeability
Term
Complement system-
Definition
serine proteases activated by antigen-antibody binding. Complement triggers A) inflammatory stimulation (C3b), B) leukocyte chemotaxis (C3a, C5a).
Term
Eicosanoids-
Definition
metabolites of arachidonic acid, prostaglandins, leukotrienes, lipoxines. Involved in inflammatory responses and tissue repair.
Term
Cytokines-
Definition
proteins that act in paracrine manner to regulate leukocyte activity. Interleukins are cykines secreted primarily by cells of hematopoietic linage.
Term
Chemokines
Definition
Term
Four phases of Inflammatory Response
Definition
Dilation of Vessels
Recruitment of cells
Chemotaxis
Phagocytosis
Term
Chronic Inflammation
Definition
Pathological state characterized by continued and inappropriate response of the immune system to the inflammatory stimulus
Term
Tissue repair
Definition
Release of growth factors and cytokines stimulating healing and scar formation through angiogenesis:
(cytokines, EGF, PDGF, TGF-β)
Term
Arachidonic Acid Pathways
Definition
Phospholipase A2 acts on the phospholipids phosphatidylcholine (PC) to release arachidonic acid.

Unesterified arachidonic acid is used as substrate for the cyclooxygenase, lipoxygenase, epoxygenase pathways.

The cyclooxygenase pathways produce prostaglandins, prostacyclin, and thromboxane.

The lipoxygenase pathways produce leukotrienes and lipoxins.

The epoxygenase pathway produces epoxyeicosatetraenoic acids (EETs).

Non-enzymatic peroxidation of arachidonic acid generates isoprostanes.
Term
Generation of Arachidonic Acid
Definition
Arachidonic acid is biosynthesized from the essential fatty acid precursor linoleic acid
Biotransformation of -linolenic acid into EPA and DHA (omega -3), also the precursors of resolvins, protectins and maresins.
Arachidonic acid is esterified to the membrane phospholipids and released from cellular phospholipids by the enzyme Phopholipase A2.
This reaction is the rate-determining step in the generation of eicosanoids.
Term
Prostaglandin Biosynthesis and Function
Definition
Cyclooxygenases are membrane bound
heme-containing enzymes

COX1 and COX2 are found in humans

Each enzyme catalyzes two sequential reactions:
Arach. A->PGG2->PGH2
Term
Cyclooxygenases
Definition
Two cyclooxygenase isoforms, COX-1 and COX-2
Term
COX-1
Definition
is constitutively expressed and is involved in “housekeeping” activities; vascular homeostasis, renal function, platelet function, and antithrombogenesis
Term
COX-2
Definition
is inducible and involves in inflammation, fever, pain, transduction of painful stimulus in spinal cord, renal adaptation to stress, uterine contraction during labor..
Term
Prostaglandins
Definition
Term
lance between TxA2 and PGI2 levels
Definition
The local balance between TxA2 and PGI2 levels is critical in regulation of systemic blood pressure and thrombogenesis
Term
Platelets express
Definition
express only high levels of thromboxane synthase

TxA2 the main eicosanoid product of platelets
TxA2 is a potent vasoconstrictor and promoter of platelet adhesion and aggregation
Term
Vascular endothelium express
Definition
express only prostacyclin synthase
PGI2 is the main ecosanoid product of vascular endothelium
PGI2 is vasodilator, and inhibitor of platelet aggregation
Term
Leukotriene Biosynthesis, and Function,
Definition
Lipoxygenase catalyse the insertion of O2 into Arach.A.
HPETEs is reduced to HETE by GSP.
HPETE formed by 5-LOX is the precursor of leukotriene A4 (LTA4)
LTA4 is the precursor of all bioactive leukotriens
5-LOX is translocated to the nuclear membrane and activated by 5-lipoxygenase activating protein (FLAP)
LTB4/LTC4/LTD4 play role in psoriasis, artheritis, vascular diseases and asthma
Term
Lipoxygenase catalyse
Definition
the insertion of O2 into Arach.A.
Term
HPETEs is reduced to
Definition
HETE by GSP.
Term
HPETE formed by
Definition
5-LOX is the precursor of leukotriene A4 (LTA4)
Term
LTA4 is the precursor of
Definition
all bioactive leukotriens
Term
5-LOX is translocated
Definition
to the nuclear membrane and activated by 5-lipoxygenase activating protein (FLAP)
Term
TB4/LTC4/LTD4 play role in
Definition
psoriasis, artheritis, vascular diseases and asthma
Term
Phospholipase A2 acts on
Definition
the phospholipids phosphatidylcholine (PC) to release arachidonic acid.
Term
Unesterified arachidonic acid is used as substrate for
Definition
the cyclooxygenase, lipoxygenase, epoxygenase pathways
Term
The cyclooxygenase pathways produce
Definition
prostaglandins, prostacyclin, and thromboxane.
Term
The lipoxygenase pathways produce
Definition
leukotrienes and lipoxins.
Term
The epoxygenase pathway produces
Definition
epoxyeicosatetraenoic acids (EETs).
Term
Non-enzymatic peroxidation of arachidonic acid generates
Definition
isoprostanes
Term
Arachidonic acid is biosynthesized from the essential fatty acid precursor
Definition
linoleic acid
Term
Biotransformation of ______ to Generation of Arachidonic Acid
Definition
Biotransformation of -linolenic acid into EPA and DHA (omega -3), also the precursors of resolvins, protectins and maresins.
Term
Arachidonic acid is esterified to the membrane phospholipids and released from
Definition
cellular phospholipids by the enzyme Phopholipase A2
This reaction is the rate-determining step in the generation of eicosanoids
Term
Lipoxin Biosynthesis
Definition
Lipoxin are derivates of arachidonic acid
Both LXA4 and LXB4 modulate the actions of leukotrienes and cytokines
Resolution of inflammation
LXA4 receptors are expressed in neutrophils, lung, spleen, and blood vessels
Lipoxin stops neutrophil chemotaxis, adhesion and transmigration, stimulate vasodilation (increase PGE2, PGI2), inhibit LTC4/LTD4-mediated vasoconstriction and inhbit LTB4 inflammatory effects.

Lipoxins stimulate the uptake and clearance of apoptotic neutrophils by macrophages-> resolution of inflammation
Lipoxin-leukotriene homeostasis may be a key factor in the pathogenesis of inflammation
Term
Lipoxin are derivates of
Definition
arachidonic acid
Term
Both LXA4 and LXB4 modulate
Definition
the actions of leukotrienes and cytokines
Term
LXA4 receptors are expressed in
Definition
neutrophils, lung, spleen, and blood vessels
Term
Lipoxin stops
Definition
neutrophil chemotaxis, adhesion and transmigration, stimulate vasodilation (increase PGE2, PGI2), inhibit LTC4/LTD4-mediated vasoconstriction and inhbit LTB4 inflammatory effects.
Term
Lipoxins stimulate
Definition
the uptake and clearance of apoptotic neutrophils by macrophages-> resolution of inflammation
Term
Resolvins, Protectins, and Maresins: Omega-3-Derived Mediators
Definition
Specialized local mediators that limit neutrophil recruitment to the site of inflammation.
Stimulate macrophage dependent clearance of apoptotic cells
Inflammation resolution is an active process
Term
The Allergic Response in Asthma
Definition
APC present the antigens to CD4+ T cells->TH2 lymphocytes.
Secreted IL-4, IL-13, and IL-5 recruit B cells and eosinophils.
The B cells differentiate into IgE-producing plasma cells.
The IgE binds to FcεRI receptors on mast cells and APC. The IgE-bound FcεRI is cross-linked, inducing the mast cell to degranulate and release inflammatory mediators including histamine, leukotrienes, platelet-activating factor, and other cytokines.
These cytokines cause acute airway inflammation.
Term
Chronically Allergic Response in Asthma
Definition
Chronically, activated TH2 cells and mast cells produce circulating IL-5 that recruits eosinophils, and TH2 cells release products that stimulate local mast cells and neurons.
Together, the inflammatory mediators and catabolic enzymes produced by eosinophils, mast cells, and neurons cause chronic airway inflammation and lead to airway remodeling.
Term
The Leukotriene Pathway in Asthma
Definition
Leukotrienes are some of the most potent bronchoconstrictors known and are important mediators of inflammation in the airway.
LTB4 is expressed in neutrophils and monocytes. It binds to BLT1 receptors expressed on leukocytes, leading to leukocyte chemotaxis, recruitment and promote cellular aggregation
LTA4 is converted to LTC4 in mast cells and eosinophils, which is transported out of the cell. LTC4 is converted to LTD4 /LTE4; all three of these cysteinyl leukotrienes bind to CysLT1 receptors expressed on airway smooth muscle cells, leading to bronchoconstriction and airway edema.

Drugs that inhibit leukotriene production or leukotriene receptor binding have a role in asthma therapy. The leukotriene pathway can be inhibited by the 5-lipoxygenase inhibitor zileuton or by the CysLT1 receptor antagonists zafirlukast.
Term
Rhemautic Arthritis
Definition
Chronic, systemic and autoimmune inflammatory disease
Primarily attacks joints, but also affects lungs, muscle and cardiovascular system
Secretion of TNF, growth factors, and IL-> induce COX-2 expression
Levels of COX-2 and PGE2 are elevated in synovial fluid of affected joints.
PGE2 stimulates pain pathways and COX-2 derivated eicosanoids and leukotrienes recruit inflammatory cells
Macrophages release collagenase and proteases that further contribute to joint damage
Common symptoms: synovitis, leukocytosis, rheumatoid nodules and the presence of rheumatoid factor (spec antibody against IgG)
Term
Proposed Roles for Tumor Necrosis Factor in Rheumatoid Arthritis
Definition
TNF is secreted by activated macrophages in an affected joint,
TNF activates endothelial cells to up-regulate expression of cell surface adhesion molecules that promote leukocyte adhesion and diapedesis.
TNF has a positive feedback effect on nearby monocytes and macrophages, promoting their secretion of cytokines such as IL-1.
IL-1 activates T cells, and the combination of IL-1 and TNF stimulates synovial fibroblasts to increase their expression of matrix metalloproteases, prostaglandins (especially PGE2), and cytokines (such as IL-6) that degrade the joint cartilage.
Synovial fibroblasts also secrete IL-8, which promotes neutrophil diapedesis
Term
Cardiovascular Disease
Definition
TxA2 is an important mediator of thrombosis in acute coronary syndrome and other cardiovascular diseases (therapeutic target)
COX-inhibitor aspirin is an effective antiplatelet agent in prophylaxis and treatment of these diseases.
Intravascular leukotriene production during rupture of atheromatous plaques also contribute to acute coronary syndrome.
5-LOX, FLAP and LTA4 are genetically linked to myocardial infarction (therapeutic targets).
Term
NSAIDs and GPIIb–IIIa antagonists inhibit
Definition
steps in thromboxane A2 (TxA2)-mediated platelet activation.
Term
Aspirin inhibits
Definition
COXs, leading to decreased TxA2 production. The platelets lack the ability to synthesize new enzyme molecules.
Aspirin is mostly used as antiplatelet agent to prevent arterial thrombosis leading to ischemic attack, stroke and myocardial infarction.
Term
GPIIb–IIIa antagonists, small-molecule antagonists inhibit
Definition
GPIIb–IIIa antagonists, such as the monoclonal antibody abciximab and the small-molecule antagonists eptifibatide and tirofiban, inhibit platelet aggregation by preventing activation of GpIIb–IIIa, leading to decreased platelet cross-linking by fibrinogen.
Term
What is the role of the innate immune system
Definition
Defend against bacterial/parasitic infections via neutralization

Recognizes non-self agent
Term
Which statement is correct for the MHC-I proteins
Definition
Term
Which combination is true for MHC-II proteins
Definition
MHC-II protein displays endocytic proteins; and has a CD4 recognition site
Term
What is true about COX enzymes:
Definition
They are involved in the prostaglandins biosynthesis from arachidonic acid
COX2 plays role in fever, pain and vasodilation
Term
Which of the following is true about cyclooxygenase pathway?
Definition
PGE2 is involved in vasodilation and bronchoconstriction
Prostaglandines are not cytoprotective

Prostacylins (PGI2) are vasodilators and promote platelet aggregation
Term
Which of the following statements are true for Leukotrienes …
Definition
Term
Which of the following cells are primarily responsible for this reaction?
Definition
Term
The action of lymphocytes attempting to eliminate this antigen is an example of..
Definition
Term
NSAIDs and GPIIb–IIIa antagonists inhibit
Definition
steps in thromboxane A2 (TxA2)-mediated platelet activation.
Term
Aspirin inhibits
Definition
COXs, leading to decreased TxA2 production. The platelets lack the ability to synthesize new enzyme molecules.
Aspirin is mostly used as antiplatelet agent to prevent arterial thrombosis leading to ischemic attack, stroke and myocardial infarction.
Term
GPIIb–IIIa antagonists, small-molecule antagonists inhibit
Definition
GPIIb–IIIa antagonists, such as the monoclonal antibody abciximab and the small-molecule antagonists eptifibatide and tirofiban, inhibit platelet aggregation by preventing activation of GpIIb–IIIa, leading to decreased platelet cross-linking by fibrinogen.
Supporting users have an ad free experience!