Term
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Definition
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Term
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Definition
| Sample color versus product color |
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Term
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Definition
| Highly purified chemical that can be measured directly to produce a substance of exact known concentration and purity |
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Term
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Definition
| Made from a primary standard |
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Term
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Definition
| Standard reference material; substances assigned a value after analysis using reference or state-of-the-art methods. |
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Term
| Beer's Law aaption formula |
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Definition
| (ABS of standard)/(Standard CONCENTRATION)= (ABS of unknown)/ (Unknown CONCENTRATION) |
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Term
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Definition
| Process by which unknown results (patients, QC) are compared to the value (output signal) initially set by the standard curve or instrument for comparison) |
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Term
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Definition
What we are trying to read
Absorbance
Emission
Chemi-bioluminescence
Flurescence
Reflection
RIA (radioactive label) |
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Term
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Definition
| Using one standard or substance with a known value in the middle of the expected range |
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Term
| Calculation of 1pt. calibration is based on |
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Definition
| Existing standard (calibration) curve and linearity verification |
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Term
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Definition
| Involves the didynium filter, holmonoxine, solutions and a color check. Must have a known maxima/ wavelength |
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Term
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Definition
| Checks for absorbance at known maxima. checks the light source and the cuvette |
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Term
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Definition
| Test by using 400nm and 700nm, measure %T, block the light path using cut-off filters or solutions |
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Term
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Definition
| Graduated to the tip. Used for transfer of the same reagent over and over. (Repeated assays/ reproducibility) |
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Term
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Definition
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Term
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Definition
| Used to deliver a particular volume of liquid. Used for accuracy and one time use. Can also be used as a calibrator or for QC |
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Term
| Are volumetric pipettes blow-out? |
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Definition
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Term
| What does Beer's Law state? |
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Definition
| That the concentration of a substance is directly proportional to the amount of light absorbed, or inversely proportional to the logarithm of the transmitted light |
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Term
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Definition
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Term
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Definition
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Term
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Definition
| Concentration of absorbing material |
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Term
| Causes of hyperproteinemia |
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Definition
Dehydration
Multiple myeloma
Increased synthesis
Plasma replacement |
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Term
| Causes of hypoporiteinemia |
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Definition
Malabsorption
Liver disease
Plasma loss |
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Term
| What does Biuret contain? |
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Definition
| Copper sulfate and sodium hydroxide to establish an alkaline environment and stabilizers to keep copper in the Cu2+ state. |
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Term
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Definition
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Term
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Definition
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Term
| Limitations to Beer's Law |
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Definition
Requires dilute solutions
Requires monochromatic incident light
Cuvette interferences
Another analyte/substance is absorbing
Any changes in the test system
Stray
Deviation in photodetector |
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Term
| What are some cuvette interferences? |
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Definition
| Scratches, dirt, fingerprints |
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Term
| If there is an anticoagulant present in the liquid portion of blood it is known as what? |
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Definition
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Term
| What is the anticoagulant present in plasma? |
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Definition
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Term
| If there is no anticoagulant present in the liquid portion of the blood it is known as what? |
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Definition
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Term
| What are some separation methods of blood? |
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Definition
Centrifuge
Barrier Gel/plastic |
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Term
| What are some sample criteria? |
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Definition
Volume
Proper anticoagulants or preservatives
Timing
Specimen is intact
Specimen been transported properly |
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Term
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Definition
| Matching of blood collection tubes with appropriate analyte request |
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Term
| Patient processing entails |
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Definition
Checking the patient id labels
Giving the sample a lab number
Readying the sample for analysis
(Bar code readers)
Separating cells from liquid |
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Term
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Definition
Hemolyzed
Icteric (orange due to bilirubin)
Turbidity/lipemia/proteinemia |
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Term
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Definition
Accounting for color of the reagent. Water replaces sample volume. (Takes abs of reagent only)
Set abs to zero with reagent blank |
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Term
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Definition
| Accounting for sample characteristcs (color of patient's sample) |
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Term
| How do you make a sample blank? |
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Definition
| Volume of sample 100ul of sample and 4.9ml of water. take abs. subtract abs from final abs |
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