Term
| ASAP. No longer than 15 minutes |
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Definition
| How quickly do we need to get the agar in the incubator after antibiotic disks have been added? |
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Term
|
Definition
| When doing a catalase test what do you need to add to your specimen? |
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Term
Bubbles=staph positive
No bubbles= staph negative |
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Definition
| A positive catalase test will show what? |
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Term
|
Definition
| If you perform a KOH test, what is the additive? |
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Term
| 1% Tetramethyl-p-phenylenediamine |
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Definition
| What is the reagent used in the oxidase test? |
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Term
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Definition
| On the MIC zone on your susceptibility test, the larger the area the lower the concentration of drug needed. True or False? |
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Term
|
Definition
| What is the reagent needed for the coagulase test? |
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|
Term
| 37 degrees C, 98.6 degrees F. |
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Definition
| What should the temperature in the incubator be for normal culturing? |
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Term
|
Definition
| When scoring a CMT,(California Mastitis test) what does the symbol T stand for? |
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Term
|
Definition
| For a CMT, at what point do you score an initial test? |
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Term
| To get a sample that represents the type of bacteria. |
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Definition
| What is the objective of specimen collection? |
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Term
|
Definition
| We have a draining tract, what are they frequently contaminated with? |
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Term
|
Definition
| Gram negative bacteria will stain what color? |
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Term
|
Definition
| Gram positive bacteria will stain what color? |
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Term
| Enterobacteria. (Lactose fermenting Enterobacteriaceae) |
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Definition
| You have a McConkey agar, it is selective media. It grows bacteria in the presence of bile salts and crystal violet. What bacteria is this good for? |
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Term
| Solid media, or agar plates. |
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Definition
| What culture media is the best and the most common used? |
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Term
| To keep the condensation off the agar. |
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Definition
| Why do we invert the agar plates when we put them in the incubator? |
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Term
|
Definition
| What is the primary isolation media? |
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Term
|
Definition
| Your enrichment broth is used to grow what? |
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Term
|
Definition
| You pull out a blood agar and have delta hemolysis. This is commonly produced by what bacteria? |
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Term
| Glucose fermentation and the production of hydrogen sulfide. |
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Definition
| The triple sugar iron agar is used for detection of what? |
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Term
| Putting a candle in the jar and closing the lid tight. This will produce carbon dioxide. |
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Definition
| How do you create an oxygen free environment? |
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Term
|
Definition
| You have a McConkey agar, you see a healthy profuse growth. This is presumptive evidence of what organism? |
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Term
|
Definition
| Urea tubes turn what color when they are positive? |
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Term
|
Definition
| Before you use a blood culture, you would use the thioglycollate broth to grow what? |
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Term
|
Definition
| How many mls are needed for a blood culture? |
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Term
| Where did the specimen come from, what area? |
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Definition
| When making the preliminary evaluation of primary cultures, what must you keep in mind? |
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Term
|
Definition
| Nosocromial infections come from where? |
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Term
| Color outside the stab line. Everything is mixed. |
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Definition
| Ho do you know if a motility test is positive? |
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Term
|
Definition
| What is it called when you are identifying microbes? |
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Term
|
Definition
| What type of organisms do we not isolate? |
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Term
| False because they need a host cell to survive. |
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Definition
| All viruses are free living. True or False and why? |
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Term
|
Definition
| If an organism is diplicoccus, what does this mean? |
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Term
|
Definition
| How do bacteria reproduce or replicate? |
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Term
|
Definition
| On a blood agar, you see green growth, what type of hemolysis is this? |
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Term
|
Definition
| Complete hemolysis is termed what? |
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Term
|
Definition
| No hemolysis is termed what? |
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Term
|
Definition
| If you use crystal violet, iodine, and saphrine, what is the name of the staining? |
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Term
|
Definition
| Bacteria prefer what pH range? |
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|
Term
Insulated walls
Strong seal
Holds plates, tubes & jars
Adjustable thermostat
-35-37 degrees F for most bacteria
-25-30 degrees F for fungi
Humidity
-70-80%
Thermometer |
|
Definition
| What must a quality incubator have inside of it? |
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Term
|
Definition
| Brucillis canins needs what type of culture? |
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Term
|
Definition
| Gram negative bacilli, aerobic and is oxidase positive. What bacteria is this? |
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Term
|
Definition
| You have a gram negative cocci, that is catalase positive, and coagulase posiutive, what bacteria is this? |
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Term
|
Definition
| You have a gram positive bacilli, it has spores but no hemolysis. What bacteria is this? |
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Term
| National Committee for Clinical Laboratory Standards |
|
Definition
| What does NCCLS stand for? |
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|
Term
| Central, subterminal & terminal |
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Definition
| What are the three categories of spores? |
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|
Term
Crustal Violet (30-60 seconds)
Rinse
Iodine (30-60 seconds)
Rinse
Decolorizing clear (10-15 seconds)
Rinse
Saphrine( 30-60 seconds)
Rinse |
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Definition
| List the gram stain in order: |
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|
Term
| To grow individual colonies of specimen. |
|
Definition
| What is the goal for streaking plates? |
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Term
|
Definition
| What bacteria grow best at low temperatures? |
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Term
|
Definition
| What are bacteria called that like to grow at high temperatures? |
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Term
|
Definition
| When describing the size, what are tiny colonies reffered to as? |
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Term
|
Definition
| This will describe the side view of a colony. |
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|
Term
Flat
Raised
Umbonmate
Crateriform
Convex
Pulvinate |
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Definition
| These are the most common types of elevation: |
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Term
|
Definition
| Most widley used inoculation media, will grow most bacteria positive and negative. Standard media selected to help identify colony morphology and hemolytic patterns. |
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Term
|
Definition
| This inoculation media provides environment similair to intestines. Selective for bacteria that grow in presence of bile salts. Gram negative only. |
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Term
|
Definition
| Least selective inoculation media. Must combine with microscopic evaluation. |
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Term
|
Definition
| This inoculation media is an exclusion test that does not allow growth of streptococcus. |
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Term
|
Definition
| A gram positive Bacilli with spores that is hemolytic is what bacteria? |
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Term
|
Definition
| A gram positive Bacilli that has no spores, is catalase positive, and motile is what bacteria? |
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Term
|
Definition
| A gram positive Bacilli that has no spores, catalase positive, and non-motile is what bacteria? |
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|
Term
|
Definition
| A gram positive Bacilli that has no spores, is catalse negative with alpha hemolysis is what bacteria? |
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|
Term
|
Definition
| A gram positive Bacilli, that is catalse negative with beta hemolysis is what bacteria? |
|
|
Term
|
Definition
| A gram negative Bacilli that is aerobic and oxidase negative is what bacteria? |
|
|
Term
|
Definition
| A gram negative Bacilli that is anaerobic is what bacteria? |
|
|
Term
| Staphylococcus epidermidis |
|
Definition
| A gram positive cocci that is catalase positive, and coagulase negative is what bacteria? |
|
|
Term
|
Definition
| A gram positive cocci that is catalase negative is what bacteria? |
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