Term
| To obtain isolated colonies of bacteria with one bacterial type |
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Definition
| What are culture media used for? |
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Term
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Definition
| Liquid or solid that supports growth |
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Term
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Definition
| These are stored at 5-10 degrees C away from fridge walls. |
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Term
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Definition
| Most common agar, has trypticase soy agar with sheep blood enriched medium. |
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Term
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Definition
| This differential medium shows up as a greenish zone. Hemolysis |
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Term
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Definition
| This differential medium show up in a clear zone. Hemolysis |
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Term
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Definition
| This differential medium will show no change. Hemolysis. |
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Term
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Definition
| This differential medium will show a double zone. Hemolysis. |
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Term
| MacConkey Agar and Eosin-Methylene |
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Definition
| This agar contains crystal violet and bile salts. |
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Term
| Eosin Methylene blue agar |
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Definition
| This agar is good for lactose-fermenting Enterobacteriaceae. |
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Term
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Definition
| This agar is good for E-Coli, Enterobacter, and Klebsiella species. |
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Term
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Definition
| This agar is used for fecal specimens. Very selective for Salmonella. |
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Term
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Definition
| This agar moderately inhibits the growth of species of bacteria that are disaccharide fermenters. |
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Term
| Selenite Broth or Tetrathionate Broth. |
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Definition
| This media is used for samples with higher concentrations of mixed bacterial species. Selectively enhances the growth of salmonella. |
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Term
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Definition
| A liquid medium used to culture anaerobic bacteria to determine the oxygen tolerance of microbes. |
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Term
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Definition
| These will grow only in oxygen rich top layer. |
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Term
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Definition
| These will grow in the lower part of the tube where no oxygen is present. |
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Term
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Definition
| Used to culture anaerobic bacteria. Also used for blood cultures. |
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Term
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Definition
| Slanted medium in a tube. |
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Term
| Sulfied-Indole Motility Tubes |
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Definition
| Tests for motility of bacteria, hydrogen sulfide production, and the break down of trytophan. |
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Term
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Definition
| Slant: Differential media that detects the presence of bacteria that use carbohydrate fermentation for metabolism that produce hydrogen sulfide. |
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Term
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Definition
| Slant Differential media that detects bacteria that produce urease. |
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Term
Gather a bacterial colony with a sterile swab
Streak the loop over 1/4 of the agar in a back and fourth motion
Re-sterilize the loop under a flame
Streak another 1/4 of the agar in the back and fourth motion, going though first streak
Repeat two more times (sterilizing in between) |
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Definition
| How do you perform the Quadrant streak method? |
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Term
| To grow individual colonies of the specimen. |
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Definition
| What is the main goal of plate streaking? |
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Term
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Definition
| This media will need to be stabbed with an inoculation needle. The needle should be inserted and removed without lateral movement to make a single tract for bacteria. |
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Term
Tilt the tube
Stab the butt
Streak from the bottom to the top. |
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Definition
| How do you perform an inoculation slant? |
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Term
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Definition
| Most agar plates are incubated inverted for how long? |
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Term
| -35-37 Degrees C for 48hrs. |
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Definition
| Most tube media is placed in the incubator immediately at what temp and for how long? |
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Term
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Definition
| Most bacteria grow best at what temperature? |
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Term
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Definition
| Bacteria that require lower temperatures. |
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Term
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Definition
| Bacteria that require high temperatures to grow. |
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Term
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Definition
| These microbes have very strict requirements and very complex nutrition. |
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Term
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Definition
Spherical cells.
Ex: Staphylococcus aureus |
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Term
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Definition
Rod shaped bacteria.
Ex- Bacillus anthracis |
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Term
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Definition
| Loose, tight spiral or comma. |
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Term
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Definition
| This refers to having both cocci or rods. |
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Term
| You have both cocci and rods. If this is in fact a pure culture, and you definitely see rods, you have bacillus. |
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Definition
| What is the pleomorphic rule? |
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Term
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Definition
| These spores occur in the center of the cell. Exp- Bacillus anthracis |
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Term
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Definition
| These spores reside near the end of the cell. Exp- Clostridum chauvoei |
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Term
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Definition
| These spores are found at the very end(pole) of the cell. |
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Term
Number of types of bacteria isolated
Relative number of each bacteria
Morphologic characteristics of each colony
Any changes in media surrounding colony. |
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Definition
| Information to note when evaluating primary cultures: |
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Term
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Definition
| Colonies that grow in the agar are usually what type of bacteria? |
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Term
| Lactose fermenting bacteria. |
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Definition
| pink to red colonies in the agar are usually what type of bacteria. |
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Term
| Bacteria not capable of lactose fermentation |
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Definition
| Colorless colonies have a slight yellowing of the media and are what kind of bacteria? |
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Term
| Disaccharide fermenting bacteria |
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Definition
| This group of bacteria will produce bright orange, yellow, salmon or pink colonies. |
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Term
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Definition
| This bacteria will produce blue-green colonies. |
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