Term
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Definition
animal inoculation
embryonated eggs
tissues cultures |
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Term
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Definition
| study pathogenesis, immune response, epidemoiology and oncongenesis. |
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Term
| Disadvantage for inoculation |
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Definition
| immunity may interfere with viral growth. |
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Term
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Definition
several sites for cultivation of viruses
chorioallantoic membrane- variola and vacccinia virus
allantoic cavity: influenzae and paramyxovirus
amniotic sac- influenza
yolk sac |
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Term
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Definition
organ culture
explant culture
cell culture |
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Term
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Definition
small bits of organs
maintained for days and weeks
tracheal ring- corona virus |
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Term
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Definition
fragments of minced tissues
adenoid tissue- adeno virus |
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Term
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Definition
tissue dissociated into component cells-protoeolytic enzymes
washed, counted and suspended in medium
constituents include amino acids, vitamins, salts, glucose, bicarbonate & 5% fetal cal serum incubated at 5% carbon dioxide. |
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Term
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Definition
| prevent bacterial contaminants |
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Term
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Definition
| cell adhere to glass surface & on incubation form a confluent growth of a single layer of cells. |
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Term
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Definition
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Term
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Definition
normal cells
limited growth
useful isolation of virus and vaccine preparation
ex: monkey kidney, human embryonic kidney, human amnion chick embryo |
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Term
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Definition
single type
sub cultivation for limited numer of times(50)
isolation of fastidious pathogens & viral vaccine production
ex: human fibroblasts |
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Term
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Definition
single type
derived from cancer cells
continuous serial cultivation
used in vaccine preparation- vero cell lines for rabies vaccine. |
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Term
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Definition
viruses cause morphological changes in cultured cells
-observed under microscope
-CPE produced by different virus and help in presumptive identification.
examples: entero - crenation of cells
measles-syncytium
herpes- discrete focal degeneration
adeno virus: large granular clumps
SV40- cytoplasmic vacuolation |
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Term
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Definition
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Term
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Definition
The CPE effect can be used to quantitate infectious virus particles by the plaque forming unit assay
cells are grown until they form a monolayer of cells
infected with virus
plaque is produced when a virus particle infect a cells then kills that cell.
cells are stained with a dye which only stains living cells
each plaque is the result of a infection of one cell by one virus followed by replication and spreading
viruses that do not kill cells may not produce plaques. |
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Term
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Definition
in normal cells medium turns acid due to cellular metabolism
virus grows-no acid production |
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Term
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Definition
Heamagglutinating virus- prescence indicated by guinea pig erythroycytes
RBA will absorb on surface- heamadsorption |
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Term
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Definition
growth of non CPA producing virus- tested by challenging with known CPE producing virus
No CPE- presence of non-pathogenic virus. |
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Term
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Definition
oncogenic virus induce cell transformation
growth- mico tumors. |
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Term
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Definition
stained by fluroescent dyes- examined for presence of viral antigens
widely used in virology. |
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Term
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Definition
small infections agents that cause disease
NA molecules without protein coat
plant viruses ss-closed circular RNA |
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Term
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Definition
infectious particle only of protein
highly resistant to inactivation by heat, formaldehyde, UV light
prion diseases- Mad cow, Kuru, scrapie in sheep. |
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