Term
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Definition
| infected animals with murine leukemia viruse and they developed tumors |
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Term
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Definition
| discovered as contaminant in cell cultures used for human poliovirus vaccines. resistant to formalin and in permissive cells it undergoes lytic. not a tumor inducing virus in its natural host |
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Term
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Definition
nonenveloped, VP1, 2, 3,
ds circular DNA with cellular histones
resistant to heat, formalin, and lipid solvent
beads on a string |
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Term
| functional regions of virus genome |
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Definition
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Term
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Definition
promoters
drive transcription of mRNA
one direction around DNA
bidirectional synthesis of mRNA
ori
poly A recognition sequence |
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Term
| overview of replication cycle |
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Definition
1. binds to receptor
2. enters cell via endocytic entry via caviolin, goes through ER, genome to nucleus
3. early gene expression; necessary to drive host cell into S phase, increase in amount of proteins necessary for DNA replication
Host RNA Pol II
4. DNA replication
5. late gene
6. assembly and release |
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Term
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Definition
Promoter for early genes encodes for mRNA which is spliced; one is large T and one small T
TATA box in promoter region binds necessary proteins to drive transcription |
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Term
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Definition
1. ori overlaps
2. sp1 binds to bp repeated region
binding controls expression of large T
3. requires specific transcription factors provided by host cell
4. enhancer region binds transcription factors |
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Term
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Definition
Binds to PP2A and inactivates PP2A
(PP2A is a phosphatase that down regulates cell signaling pathways. mitogen-activated kinase pathway)
stimulates transcription |
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Term
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Definition
when MAP gets activated it phosphorylates AP1 (AP1 is cellular transcription factor)
AP1 binds to promoters and stimulates transcription |
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Term
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Definition
Binds to Rb
E2F is dissociated and binds to DNA
Drives cell to S phase
can inactivate p53 tumor suppression |
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Term
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Definition
transcription factor
DNA damage leads to rapid induction of p53
promotes G1/S arrest
apoptosis
stops cell from dividing to allow repair |
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Term
| p53 activates transcription of ______ |
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Definition
p21
p21 blocks cell cycle progression |
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Term
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Definition
1. Large T binds to ori and allows bidirectional replication
2. recruites cellular polymerase
3. association of topoisomerase to introduce nicks in DNA to relax to continue replication
4. Large T forms 2 hexamers and binds to specific sites in ori. prevents further expression of early genes
5. has helicase acitivity and associates with DNA pol |
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Term
| Replication vs. transformation |
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Definition
1. permissive cells allow viral replication and then lysis
2. nonpermissive cells don't allow viral replication. primase does not inteact correctly. no change from early gene to DNA replication. causes over abundance of large and small T--transofrmation because p53 inactivated and causes tumor and cancer |
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Term
Mouse polyomavirus
encodes how many antigens?
and which one intitates transformation? |
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Definition
three antigens (L,S,M)
middle interacts with membrane proteins to initiate cellular transformation
forces Src (Tyr kinase) into active conformation
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Term
SV40
Transmission/shed
symptomatic? |
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Definition
inhalation and shed in urine (kidney)
in natural host (primates): persistent and asymptomatic
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Definition
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