Term
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Definition
| classifying virus based on genome type and the polymerases that go with them |
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Term
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Definition
| POLIO,SINDBIS, Murine Hepatitis, MS2, Qbeta |
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Term
| Picornaviridae meaning and virus in it |
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Definition
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Term
| Genome type for and shape polio |
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Definition
| SS + RNA, icosahedral (looks like typical PHAGE) |
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Term
| # of coat proteins for polio and 4 functions |
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Definition
| 4 proteins (34k,30k,26k,7k), protects RNA, Has specific receptor, determines host range and tissue tropism, delivers and disgorges DNA |
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Term
| Polio virus RNA description |
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Definition
| poly A 3', viral protein genome on 5' end, 7435 NT's long, one open reading frame, 740 NT's non coding on 5'which is leader and 80 on 3'terminal |
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Term
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Definition
| one step growth in 5-10 hours, .1-2% plaque forming efficiency, mutates every 10-4 per base position |
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Term
| Polio Virus adsorbtion, penetration, etc...how does get there |
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Definition
| Receptor mediated endocytosis, acidification of endosome, conformational change of capsid including loss of v4 |
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Term
| Steps for Polio virus genome replication |
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Definition
1.)form polysome on incoming RNA
2.)form polyprotein which is cleaved during translation into 3 segments (p1,p2,p3)
3.)Intiation of replication on smooth cytoplasmic membranes in a replication to make a dsRNA intermediate
4.)Intitiation occurs at 3' end of the + RNA poly-A tail and the genome is held circular by virus and Host proteins
5.)Loosely held antigenome is only found conected to genome and allows for up to 5 multiple initiations
6.)newly synthesized antigenome is used to make more genome with Vpg attached to 5' end |
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Term
| effect polio has on cell and why |
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Definition
| shuts down translation of host mrna by cleaving eIF 4G in cap binding complex (no 5' for polio) |
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Term
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Definition
| -RNA synthesis doubles every 15 minutes at first but then but then makes up 10% at end. As amount of proteins made increases amount of + RNA gets packaged at according rate |
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Term
| Sindbis virus family and general structure |
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Definition
| togaviridae, 5' cap poly adenylated + mrna, icosahedral enveloped, 2 open reading frames |
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Term
| sindbis virus eclipse phase steps |
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Definition
1.)translation of first 7,600 bases for polyprotein that is cleaved
2.)formation of 49s antigenome from 49s genome |
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Term
| what occurs for translation of 26s mrna in sindbis virus |
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Definition
| c is cleaved on it to give hydrophobic signal, which is bound to signal particle receptors in host cell. |
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