Term
|
Definition
cocci
white/golden opaque colonies
form grapelike clusters by dividing in 2 dimensions |
|
|
Term
|
Definition
Gram Positive
facultative anaerobes (catalase positive)
non motile
salt resistant and fermentative (mannitol)
coagulase: positive tend to be more pathogenic; can also be negative
can be hemolytic |
|
|
Term
|
Definition
staph aureus: protein binds to prothrombin to form staphylothrombin
non proteolytic activation of prothrombin
catalyse fibrinogen -> fibrin
other Staph spp: non specific proteolysis or coagulase homologs
role of coagulase in virulence has not been proven; coagulase activity is associated w more pathogenic spp |
|
|
Term
examples of coagulase positive staph |
|
Definition
s aureus
s pseudointermedius
s intermedius
s hyicus
s delphini
|
|
|
Term
examples of coagulase negative staph |
|
Definition
s epidermis
s hemolyticus
s simulans
many more
NORMAL FLORA! |
|
|
Term
virulence factors: cell surface |
|
Definition
teichoic/lipoteichoic acid (same as all Gram +)
Ig binding protein: PROTEIN A
fibronectin/fibrinogen binding (attach to epithelium and endothelium)
heme binding proteins: sequester Iron (ties in w hemolysis)
capsule (not that much)
accessory gene regulators aka Quorum sensing (colonies and biofilms)
|
|
|
Term
virulence factors: extracellular |
|
Definition
DNAase
phospholipase
staphylokinase (breaks up fibrin - keeps infections from being "walled off" by the body)
hyaluronidase (inc tissue permeability)
alpha toxin: hemolysin
leukocidin
exfoliative toxin (spreading)
pyrogenic endotoxins (fever)
TSS toxins: superantigen
enterotoxins: food poisoning
|
|
|
Term
penicillinase mediated resistance |
|
Definition
plasmid in s aureus, chromosomal in other spp
can't treat with penicillin, but could use other beta lactams
semisynthetic penicillin is also resistant to penicillinase |
|
|
Term
|
Definition
present in multiple species (not just s aureus)
chromosomal mecA gene (MEChanism of Aquisition unknown)
encodes penicillin binding protein PBP 2a
low binding affinity for ALL beta lactam subclasses
multi drug resistance in other classes can occur (MRSA)
|
|
|
Term
s. aureus affected species |
|
Definition
human, bovine, sm ruminants, pigs, horses, dogs, cats, birds |
|
|
Term
s pseudointermedius affected species |
|
Definition
dog, cat, occasionally others
used to be classified w s. intermedius |
|
|
Term
|
Definition
pigs, occasionally cattle
"greasy pig disease!"
endogenous infection |
|
|
Term
|
Definition
endogenous: common (opportunistic infection)
animal to animal
contaminated fomites
nosocomical: resistant strains |
|
|
Term
|
Definition
phage typing: some bacteriophages are specific to the level of the bacterial strain
molecular epidemiology (PCR) |
|
|
Term
|
Definition
acute pyogenic infections most common
usually involve the skin
can cause septicemia
chronic infections can also occur (osteomyelitis, botryomycosis - cattle) |
|
|
Term
epidermis: barrier factors |
|
Definition
thin layer of epithelial cells that constantly slough off
keratin
dryness
high salt content
lipids (toxic and seal the surface)
organic acids
resident flora compete for nutrients
antigen presenting cells, lymphocytes: local immune response
keratinocytes: produce cytokines |
|
|
Term
|
Definition
thicker
connective tissue
blood supply (immune)
hair follicles
sebaceous glands
sweat glands
pH 3-5 (low)
fatty acids |
|
|
Term
|
Definition
can be resident or transient
mostly gram positive bacteria and yeasts
|
|
|
Term
common risk factors for staph infection |
|
Definition
break in epithelium
moisture retention: folds
exocrine or endocrine disorders (glands don't secrete)
altered microflora (lose competition)
keratinization defects |
|
|
Term
|
Definition
superficial pyoderma - impetigo, superficial folliculitis
deep pyoderma (folliculitis, BOIL, cellulitis, subcutaneous abcess)
hoof infections
outer ear (otitis externa)
mastitis
vascular compromise leading to skin issues |
|
|
Term
|
Definition
diffuse inflammation of subcutaneous layer
rapidly advances along tissue planes
associated w exotoxin producing bacteria
|
|
|
Term
|
Definition
risks: thin stratum corneum
lack of follicular plug
epidermal barriers don't develop well enough
ex keratin, glands, acids, lipids, immune |
|
|
Term
|
Definition
drain abcess (ABx not very effective if abcess has formed)
treat topically or systemically with antimicrobials |
|
|
Term
|
Definition
culture
serology uncommon (bovine)
|
|
|