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Vet Lab Procedures
Covers all topics up to the midterm review
81
Veterinary Medicine
Undergraduate 1
06/17/2010

Additional Veterinary Medicine Flashcards

 


 

Cards

Term

Can be refrigerated

NEVER frozen

Definition
Whole Blood
Term
The fluid portion of whole blood in which the cells are suspended
Definition
Plasma
Term
The liquid portion of clotted blood
Definition
Serum
Term
Can be refrigerated or frozen
Definition
Plasma & Serum
Term
Leukocyte
Definition
White Blood Cell
Term
Erythrocyte
Definition
Red Blood Cell
Term
Thrombocyte
Definition
Platelet
Term
Leukocytes that contain granules in their cytoplasm
Definition

Granulocytes:

 

  • Neutrophils
  • Eosinophils
  • Basophils
Term
Leukocytes that do not contain granules in their cytoplasm
Definition

Agranulocytes:

 

  • Monocytes
  • Lymphocytes
Term
Each RBC has how many hemoglobin molecules?
Definition
400 Million
Term
[image]
Definition
Hemolyzed serum/plasma sample
Term
[image]
Definition

Icteric serum/plasma sample

 

Caused by impaired liver function, allowing the buildup of the yellow pigment bilirubin

Term
The normal appearance of a serum/plasma sample
Definition
Clear to Straw in color
Term
A serum or plasma sample that is cloudy or milky in appearance is...
Definition

Lipemic

(excess fat in the bloodstream)

Term
No additive
Definition
Red top
Term

Lab Uses:

Serum

General chemistry analysis

Definition
Red or Red & Gray Stripe top
Term

Additive:

Clot activator & gel serum separator

Definition
Red & Gray Stripe top
Term

Lab Uses:

Whole Blood

Plasma

Definition
Lavender or Green top
Term

Lab Uses:

Whole blood

CBCs

Routine hematology

Plasma

Specific chemistry tests (ACTH)

Not suitable for general chemistry analysis

Definition
Lavender top
Term

Additive:

EDTA (anticoagulant)

Definition
Lavender top
Term

Lab Uses:

Whole blood

Enzyme testing (HW, FeLV)

CBCs on avian blood

Plasma

General chemistry analysis

*Sodium heparin is used for IV fluid therapy

Definition
Green top
Term

Additive:

Sodium or Lithium Heparin

(anticoagulant)

Definition
Green Top
Term

Additive:

Sodium citrate

(anticoagulant)

Definition
Blue top
Term

Lab Uses:

Platelet studies

Can be used for blood transfusion

Definition
Blue top
Term

Additive:

Diatomaceous earth

Definition
Gray top
Term

Lab Uses:

Activated Clotting Time Test

Definition
Gray top
Term
Instrument used to measure the dissolved solid content of substances
Definition
Refractometer
Term
Normal range for Total Protein
Definition
5.94 - 7.82 g/dL
Term
A correctly calibrated refractometer should read what using distilled H2O?
Definition
1.000
Term
Positively charged particle inside the nucleus of an atom
Definition
Proton
Term
Uncharged particle within the nucleus of an atom
Definition
Neutron
Term
Negatively charged particle that orbits around the nucleus of an atom
Definition
Electron
Term
The number of electrons is equal to the number of what to make the atom neutral?
Definition
Protons
Term
What is the number of protons in the atom of an element called?
Definition
The atomic number, listed above its symbol on the periodic chart
Term
Inert
Definition
When an atom's outermost shell is completely filled
Term
Covalent Bond
Definition
A bond between two atoms sharing electrons to fill their outermost shells
Term
Ionic Bond
Definition
A bond between atoms that have transferred electrons from one to the other, creating an electrical (positive/negative) attraction to each other
Term
Ion
Definition
An atom with an electrical charge
Term
Cation
Definition
A postively charged ion
Term
Anion
Definition
A negatively charged ion
Term
Electrolyte
Definition

Molecules that separate into ions when they are dissolved in water.

Found in various body fluids, especiallly plasma.

Term
Na+
Definition
Sodium
Term
K+
Definition
Potassium
Term
Cl-
Definition
Chloride
Term
HCO3-
Definition
Bicarbonate
Term
Ca++
Definition
Calcium
Term
Mg++
Definition
Magnesium
Term
PO4-
Definition
Phosphate
Term
Primary functions of electrolytes
Definition

1. Maintain water balnce in the various body compartments through osmotic pressure

2. Maintain constant pH (acid-base regulation)

3. Activation of blood coagulation and enzyme systems

4. Maintain normal muscular and nervous functions

Term
Osmosis
Definition
The passage of water through a semi-permeable membrane from a dilute solution to a more concentrated one. Water will always travel towards a higher concentration.
Term
Osmosis is primarily controlled by which electrolyte(s)?
Definition
Sodium & Chloride
Term
Isotonic
Definition
The concentration is the same inside the cell as outside the cell
Term
Hypotonic
Definition
The concentration is lower outside the cell than inside, so fluid will trav4el from outside the cell into the cell
Term
Hypertonic
Definition
The concentration is higher outside the cell than inside, so fluid will travel from inside the cell out into the surrounding solution. This often gives the cell a creanated appearance.
Term
Acid
Definition
A substance that releases hydrogen ions (H+) when dissolved in water
Term
Base
Definition
A substance that releases hydroxide ions (OH-) when dissolved in water
Term
Salt
Definition

A substance composed of the postive ion of a base and the negative ion of an acid.

 

Ex: Hcl + NaOH --> NaCl + H2O

Term
Acid - Base balance within the body is primarily affected by which electrolyte(s)?
Definition
Bicarbonate (HCO3-)
Term
Neutral pH
Definition
7.0
Term
pH above 7.0
Definition
Basic (alkaline)
Term
pH below 7.0
Definition
acidic
Term
Normal pH for Blood
Definition
7.35 - 7.45
Term
Normal pH for urine
Definition
5.0 - 8.0
Term
Normal pH for stomach acid
Definition
1.0 - 4.0
Term
Normal pH for intestinal tract
Definition
8.0 - 10.0
Term
Amino acid
Definition

Organic substance (proteins) made of these four molecules:

carbon

hydrogen

oxygen

nitrogen

Term
Duration of Anestrus
Definition
Average 4.5 months
Term
Anestrus signs
Definition
Female does not attract the male. No vulvar swelling or discharge.
Term
Anestrus Cytology
Definition

Acellular

Predominately noncornified squamous epithelial cells

May also contain some neutrophils

Term
Proestrus signs
Definition

Female attracts male but will not allow breeding

Swollen vulva with reddish discharge

Term
Proestrus duration
Definition
Average 7 - 9 days
Term
Proestrus cytology
Definition
Predominately noncornified squamous epi cells which start to change to cornified cells as the cycle approaches the estrus stage. Neutrophil numbers decrease and RBCs present. Bacteria may be present.
Term
Estrus signs
Definition

Female attracts male and will allow breeding.

Swollen vulva, with straw colored discharge.

Term
Estrus duration
Definition
Average 7 - 9 days
Term
Estrus cytology
Definition
Predominately cornified squamous epi cells. Neutrophils absent, RBCs decrease. Almost 100% cornification. May be some sheeting of cells during last 1 - 2 days. Background should look very clean.
Term
Diestrus (Metestrus) Signs
Definition
Female no longer attracts the male and is not receptive to breeding
Term
Diestrus (Metestrus) duration
Definition
Average 2 - 3 months
Term
Diestrus (Metestrus) cytology
Definition
Cornified squamous epi cells are replaced by noncornified epi cells and cytologic debris.
Term
Cystitis
Definition

lower urinary tract infection (bladder)

will see WBC's and bacteria

No casts

Term
Pyelonephritis
Definition

upper urinary tract infection (kidney)

will see WBC's and bacteria

casts will be present

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