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Unit 4 Exam
N/A
74
Microbiology
Undergraduate 2
06/11/2011

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Term
The genetic information in a cell
Definition
genome
Term
What contains a cell's genome?
Definition
The chromosome
Term
What are genes?
Definition
segments of DNA
Term
What are the extrachromosomal pieces of DNA found in bacteria?
Definition
Plasmids
Term
What does haploid mean?
Definition
One
Term
Describe the bacterial chromosome
Definition
Single circular strand of DNA
1 per cell
Contained in nucleoid due to a lack of a nuclear membrane
NOT associated with histone proteins
Term
Describe Eukaryotic chromosomes
Definition
Peripheral
Exist in pairs (diploid)
Exist as linear molecs of DNA
Term
Eukaryotic extrachromosomal DNA can be found in ______ and _______
Definition
Mitochondria; chloroplasts
Term
What are the monomer units of DNA?
Definition
nucleotides
Term
What nitrogen base is ONLY present in RNA?
Definition
Uracil
Term
What are the three parts of a nucleotide?
Definition
Phosphate group, sugar, and the nitrogenous base
Term
What is the formula for the pairing rule?
Definition
%(A+T) + %(G+C)= 100%
Term
DNA is a ____ molecule: cut in half lengthwise, the DNA runs in opposite directions
Definition
antiparallel
Term
Who discovered the double helix shape of DNA?
Definition
Watson and Crick
Term
____ synthesizes new DNA using one of the parental strands as a template
Definition
DNA polymerase
Term
DNA replication is semi-_______
Definition
conservative (one original strand, one daughter strand)
Term
What makes RNA different from DNA?
Definition
Ribose is the sugar
Uracil replaces Thymine
SINGLE STRANDED molecule
SHORTER
Term
What are the locations of RNA in eukaryotic cells?
Definition
nucleus and cytoplasm
Term
What are the location of DNA in eukaryotes?
Definition
nucleus, mitochondria, or chloroplasts
Term
What are the different forms of RNA in prokaryotes?
Definition
mRNA, rRNA, and tRNA
Term
Identify:
The synthesis of a complementary strand of RNA from a DNA template
Definition
Transcription
Term
Describe the PROCESS of transcription
Definition
mRNA carries the coded information from DNA to the ribosomes where proteins are synthesized
Term
Identify:
Uses the enzyme RNA polymerase and RNA nucleotides from the cytoplasm for construction of the mRNA molecule
Definition
Transcription
Term
What is the "language" of mRNA?
Definition
Codons
Term
What is the "start" codon for mRNA?
Definition
AUG
Term
What types of codons code for amino acids?
Definition
Sense codons
Term
What is another name for "stop" codons?
Definition
nonsense codons
Term
What is the site of translation?
Definition
The ribosome
Term
What type of bonds join AMINO ACIDS?
Definition
peptide bonds
Term
Where does transcription occur in eukaryotes?
Definition
the nucleus
Term
True or false:
Prokaryotes have introns AND exons
Definition
False; only exons
Term
____ will remove the intron derived RNA and splice together the exon derived RNA To produce mRNA
Definition
Ribozymes
Term
Conjugation requires cell-to-cell ____
Definition
contact
Term
What kind of plasmid codes for the synthesis of sex pili
Definition
Gram -
Term
Conjugating cells must generally be of the _____ mating type
Definition
opposite
Term
What does Hfr stand for?
Definition
high frequency of recombinatino
Term
Define bacteriogenic
Definition
contain genes that synthesize toxic proteins that kill other bacteria
Term
What type of plasmid code for enzymes that trigger catabolism of unusual sugars and hydrocarbons?
Definition
Dissimilation plasmids
Term
Define transformation
Definition
Genes are transferred from one bacteria to another as "naked DNA"
Term
What are the three mechanisms of gene transfer?
Definition
transformation, conjugation, transduction
Term
Agents in the environment that directly or indirectly bring about mutations are called what?
Definition
mutagens
Term
What is the purpose of the Ames test?
Definition
to measure the reversion of histadine auxotrophs
Term
_____ uses bacteria as carcinogen indicators
Definition
the Ames test
Term
What are the different types of point mutations?
Definition
silent/neutral, missense, and nonsense
Term
a(n)___ has a nutritional requirement that is absent in the parent
Definition
auxotroph
Term
Identify:
when the mRNA is translated into protein the incorrect base may cause the insertion of the incorrect amino acid
Definition
Missense
Term
Identify:
creates a stop codon in the middle of an mRNA molec.
Definition
Nonsense codon
Term
What is genetic engineering?
Definition
A process by which genes are inserted into cells
Term
____ is where RNA polymerase initiates transcription
Definition
Promotor site
Term
Define transgenic
Definition
organisms that contain genes from another species to improve food quality and productivity
Term
____ lakes have deep, clean water, low BOD, high DO and high amount of game fish
Definition
Oligotrophic
Term
_____ lakes have shallow, murky water, high BOD, low DO
Definition
Eutrophic
Term
Identify:
The animal life of the plankton family
Definition
zooplankton
Term
What is the predominant fecal coliform?
Definition
e. coli
Term
____ water is safe to drink
Definition
potable
Term
Drinking water is obtained from either ___ ____ or _____
Definition
surface reservoirs or aquifers
Term
Primary treatment removes _____ ______
Definition
biodegradable matter
Term
What is the purpose of secondary treatment?
Definition
to remove organic matter thereby reducing BOD (biological oxygen demand)
Term
salmonella typhii
invades gall bladder leading to chronic carriers (typhoid mary)
Humans are the exclusive host
Definition
typhoid fever
Term
vibrio cholera
3-5 gallons diarrhea/day
best treatment is oral electrolyte therapy
Definition
Cholera
Term
shigella flexneri
Diarrhea with mucous and blood
Large intestine is the primary site of the disease
Definition
bacillary dysentery
Term
Entamoeba histolytica usually from unsanitary food/water treated with antidiarrheal meds
Definition
amoebic dysentery
Term
giardia lamblia
distinctive odor of H2S gas in breath and greasy stools
Common in wilderness water sources
Definition
giardiasis
Term
fecal-oral
virus can survive several days outside of the body
vaccine available
Definition
Hepatitis A
Term
E. coli
part of normal flora
watery diarrhea with low grade fever
Definition
Traveler's diarrhea
Term
Salk vaccine is most recommended
Sabin vaccine is oral
virus that has 3 serotypes
Definition
Poliomyelitis
Term
cryptosporidium parvum
protozoan that causes severe watery diarrhea
can be fatal in immunocompromised patients
Definition
Cryptosporidium
Term
What are saprophytes?
Definition
decomposers
Term
symbiotic organisms that consist of algae and fungi
Definition
lichens
Term
what are the pioneer organisms of barren rock to soil formation?
Definition
lichens and cyanobacteria
Term
_____ uses microbes to detoxify or degrade pollutants
Definition
bioremediation
Term
_____ makes use of certain microbes selected for growth on petroleum products
Definition
bioaugmentation
Term
_______ creates a diamond shaped crystal toxic to insects that ingest it
Definition
Bacillus thuringiensis
Term
What greenhouse gas is more potent than carbon dioxide?
Definition
methane
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