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Unit 1
Intro to Microbiology
44
Microbiology
Undergraduate 2
12/11/2010

Additional Microbiology Flashcards

 


 

Cards

Term
Robert Hooke

"Hook - Look
Definition
Coined the term "cell Theory"
Invented first reflecting microscope
Term
Anton van Leeuwenhoek

"Le" - Lens
Definition
Master of the Lenses...
First to see unicellular organisms
Animalcules
Term
Louis Pasteur
Definition
Invented antiseptic Technique
Helped with Germ theory of disease
Created first vaccines for anthrax and Rabies
Term
Joseph Lister
Definition
Introduced sterilization with antiseptic
Introduced "Germ Theory of Disease"
Term
What is "germ theory of disease"
Definition
That disease is caused by tiny, invisible organisms
Term
Robert Koch

Discribe his theory (4)
Definition
Koch's Postulates
1. organism must be present
2. must be able to isolate
3. pure culture must cause disease in new host
4. must be able to isolate again in new host
Term

Edward Jenner

clue - (Jr.)

Definition
Discovered Vaccine - "small pox/cow pox" Experimented on his own son
Term
Alexander Flemming
Definition
Discovered penicillin
Also, first to warn about over-use
Term
What are the 3 domains
Definition
Eukarya
Bacteria
Archae
Term
What are the kingdoms in Eukarya
Definition
Animalia
Fungi
Plantae
Term
Use domain system...
E. coli
E is ??
Coli is ??
Definition
Escherichia = Genus
coli = Species
Term
What are strains?
Definition
Slight variations of Species
Term
Diff b/w classification and indentification
Definition
Class. - in depth, ie: DNA sequesnicing
Iden. - fast, practicle techniques
Term
What is morphology
Definition
Cell shape, color, growth patterns, etc
Term
Why are stains helpful for looking for bacteria
Definition
Cheap, fast, easy, broad
Term
What are biochemical tests good to test for
Definition
Sugar break-down
Fermentation
Anaerobic/aerobic
Indentifying enzymes
Term
What is Serology
Definition
Study of serum and immune responces
Term
What are antibodies
Definition
Proteins made by the immune system
Term
Describe PCR
Definition
Polymerase Chain Reaction
Rapid technique that can test for the presence of specific DNA sequences from tiny amounts of DNA
Term
Protons & Electron
Definition
Protons +
Electrons -
Electrons can change the charge, protons do not change
Term
Define an Atom
& Molecule
Definition
Atom = Irreducable, furthest we can break down
Molucule = 2 or more atoms
Term
Covalent bond
Definition
Strongest - sharing of paired electrons
Term
Describe Ionic bond
Definition
formed by the attraction of 2 opposite charge ions
Term
Hydrogen bond
Definition
partial opposites attracted toward each other
Term
List the six elements that make up most matter on earth
Definition
Oxygen
Hydrogen
Nitrogen
Carbon
Sulfur
Phosphorus
Term
What are the 4 major macromolecules in biology
Definition
Carbohydrates - crucial to cell development
Lipids - Long C/H chain, denses form of energy
Amino Acids - makes proteins
Nucleotides - makes DNA/RNA
Term
Define Hypertonic
Definition
Something having greater number of solutes
Term
Define Hypotonic
Definition
Solution having lower solute level
Term
Does bacteria have cell walls? If so, what are they made out of?
Definition
Yes. Peptidoglycan

(a few bacteria don't, such as mycoplasma)
Term
Gram Positive cell wall
pros & cons
Definition
Pros - protects against shearing; resists drying out
Cons - Exposed cell wall vulnerable to penicillin
Term
Gram Negative cell walls
Pros & Cons
Definition
Pros - more resistant to immune attack
Cons - Vulnerable to drying
Term
How does the gram stain work
Definition
In the gram neg, the outer thin peptidoglycan wall is washed away with the two dyes - CV & iodine; unlike the gram positive that retains the dye in the thick peptidoglycan cell wall
Term
Define LPS
Definition
Lipopolisacchirides - Hydrophilic outer layer found on gram neg bacteria that produce endotoxins
Term
Diff b/w endotoxins and exotoxins
Definition
exotoxins are toxic substances secreated by bacteria and released outside of the cell. whereas endotoxins are bacterial tocins composed of lipids that are within a cell
Term
What are polysaccharides
Definition
Sugary proteins surround the outside of the cell, either a capsule or Slime layer
Term
Pili
Definition
Used in cell conjugation
Term
What are important targets of antibiotics?
Definition
Ribosomes (they create proteins)
Term
what are inclusions?
Definition
Spot in a cell where it piles up a lot of something: lipids, sulfur, gas, etc.
Term
Define Halophiles
Definition
Organisms that can survive in high concentrations of salt
Term
Define Psychotrophs
Definition
can grow and replicate in very cold temps
Term
Define Mesophiles
Definition
Grow in moderate temps
Term
Define Thermaphiles
Definition
Thrives in high temps
Term
Acidophiles
Definition
prefer acidic solutions
Term
Alkalipjiles
Definition
prefer basilic environments
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