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Types of Media - Selective and Differential Media
Part 2: Different types of selective and differential media, and identification of microorganisms depending on growth or colour change
31
Microbiology
Undergraduate 2
10/11/2012

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Term
What does Kliger's Iron Agar test for?
Definition
ability to ferment glucose and/or lactose, H2S production, and gas production
Term
If the bacteria contains the enzyme cysteine desulfurase, what can you expect from the KIA tube?
Definition
black precipitate
Term
How can gas fermentation be determined in a KIA tube?
Definition
cracks or lifting of the slant
Term
What is OF Glucose used to determine?
Definition
if bacteria can use glucose in an oxidative (aerobic) or fermentative (anaerobic) condition
Term
Which two tests is E. coli positive for?
Definition
Indole and Methyl Red
Term
Which two tests is E. aerogenes positive for?
Definition
Voges-Proskauer and Citrate
Term
For a motility media test, what is the indicator for growing bacteria?
Definition
tetrazolium chloride
Term
What indicates growth on motility media?
Definition
red
Term
What indicates motility in motility media?
Definition
red colour away from inoculation
Term
What colour is a Bismuth Sulfide Agar (BSA)?
Definition
dull green
Term
What microorganism produces a black or very dark brown colour on BSA?
Definition
Salmonella typhi
Term
What is brilliant green agar (BGA) used to indicate?
Definition
lactose/sucrose fermentation
Term
What colour will lactose/sucrose fermenting organisms turn BGA agar?
Definition
yellow or yellow-green
Term
What colour will non-lactose/sucrose fermentors produce in BGA?
Definition
opaque red/pink/white colonies and turn surrounding media red
Term
What colour will Salmonella turn SS agar?
Definition
black
Term
What colour will Shigella colonies be on SS agar?
Definition
colourless
Term
If a colony is lactose positive on SS agar, what colour will it appear?
Definition
red
Term
What does desoxycholate citrate test for?
Definition

gram neg and lactose neg microorganisms

(some lactose + will turn red)

Term
What happens to the plasma in a coagulase positive test?
Definition
solidifies
Term
What does Phenol Red Manitol Salt Agar (MSA) test for?
Definition
Staphylococcus
Term
What is the salt concentration in both MSA and Staph 110?
Definition
7.5%
Term
What colour will the MSA plate and colonies turn if the organism is mannitol positive?
Definition
yellow
Term
What colour will the Staph 10 medium and colonies turn if it is mannitol positive?
Definition
none; it will have a natural colour
Term
What does DNAse test for?
Definition
exoenzyme DNase
Term
What can be observed in a DNAse plate if the microorganism is DNase positive?
Definition
zone clearing
Term
What can be added to DNase to see a positive DNase test?
Definition
0.1N HCl
Term
What does M-staphylococcus contain?
Definition
10% NaCl
Term
What does M-staphylococcus broth select for and why?
Definition
Staphylococcus, because staph prefers high salt concentration
Term
What gram type does Endo agar select for?
Definition
gram negative
Term
What is endo agar differential for?
Definition
lactose
Term
What colour are the colonies and the surrounding medium in a lactose positive Endo agar?
Definition
red
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