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Types of Media - Selective and Differential Media
Part 1: Different types of selective and differential media, and identification of microorganisms depending on growth or colour change
17
Microbiology
Not Applicable
10/10/2012

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Cards

Term
What gram-type microorganisms does phenylethyl alcohol agar (PEA) select for growth?
Definition
gram positive
Term
What gram type organism does phenylethyl alcohol inhibit?
Definition
gram negative
Term
What gram type microorganism does desoxycholate agar (DES) select for growth?
Definition
gram negative
Term
What gram type microorganism does deoxycholate agar inhibit?
Definition
gram positive
Term
What type of fermentor does deoxycholate agar (DES) differentiate?
Definition
lactose
Term
What do lactose fermentors produce to precipitate bile salts in the media?
Definition
acid
Term
How do lactose positive microorganisms appear their respective colour in the DES plate?
Definition
precipitated bile salts absorb the neutral red dye from the DES plate
Term
What gram type microorganism does Eosin Methylene Blue (EMB) select for?
Definition
gram negative
Term
In EMB plates, which produces more acid: mixed acid fermentors or butanediol fermentors?
Definition
mixed acid fermentors
Term
In EMB plates, what colour are mixed acid fermentor colonies?
Definition
colonies with dark-blue black centers
Term
In EMB plates, what mixed acid fermentor produces a metallic green sheen?
Definition
Escherichia coli
Term
In EMB plates, what colour are the butanediol fermentor colonies?
Definition
pale pink to lavender centers
Term
In EMB plates, what butanediol fermentor will not produce a metallic green sheen?
Definition
Enterobacter
Term
What type of media differentiates microorganisms based on their reactions on blood?
Definition
Blood agar
Term
What type of hemolysis presents no blood hemolysis and no zone clearing around the colony?
Definition
gamma
Term
What type of hemolysis presents complete blood hemolysis and complete clearing around the colony?
Definition
beta
Term
What type of hemolysis presents partial blood hemolysis and partial clearing around the colony?
Definition
alpha
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