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Topic 16
DNA Replication
16
Biology
Undergraduate 1
04/14/2012

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Term
Replication Direction
Definition
Replication goes from 3' to 5' and constructs new strand from 5' to 3'.
Term
Origins of Replication
Definition
Points at were replication begins.
Term
Semiconservative Replication
Definition
Both DNA strands are replicated.
Term
Topoisomerase
Definition
Unwinds double helix.
Term
Helicase
Definition
Separates DNA strands.
Term
Single-Strand Binding Protein
Definition
Prevents DNA strands from rejoining.
Term
DNA-Polymerase III (DNApol III)
Definition
Constructs new DNA strand. Can only add nucleotides to an existing strand. Proof-reads inserted bases.
Term
Primase
Definition
Constructs RNA primed to which DNApol III can add nucleotides.
Term
Leading Strand
Definition
3' - 5' constructed continuously.
Term
Lagging Strand
Definition
Requires extra RNA primers for construction and is constructed in fragments (known as Okazaki fragments).
Term
DNApol I
Definition
Replaces RNA primers with DNA.
Term
DNA Ligase
Definition
Joins DNA fragments together.
Term
Differences Between Prokaryotic and Eucaryotic Replication
Definition
Prokaryotes have mitple origins of replication, Pol α creates new strand, Pol β replaces RNA primers with DNA.
Term
Endonucleases
Definition
Check and remove incorrect bases from DNA (polymerase then inserts correct bases).
Term
Excision Repair
Definition
Part of DNA is cut out and replaced (such as thymine dimers caused by UV light).
Term
Polymerase Chain Reaction (PCR)
Definition
Method of replicating DNA in the lab by separating it with heat and then adding DNApol and bases (this sequence is repeated as many times as needed).
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