Term
| 1. The embryonic stem cell line, SCNT-hES-1, was made by |
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Definition
| D. enucleating an egg and replacing the nucleus with a cumulus somatic cell. |
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Term
2. In theory the oocyte's cytoplasm would reprogram the egg's nucleus by
____________ all the somatic cell genes and ___________ the embryonic
ones. |
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Definition
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Term
| 3. Instead of sperm activation of the egg, activation was achieved by |
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Definition
| C. addition of the ionophore A23187 and DMAP. |
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Term
| 4. All of the following statements are true about SCNT-hES-1 EXCEPT |
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Definition
| B. it spontaneously formed dopamine-producing neuroectoderm cells at a very low (1%) level |
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Term
| 5. The scientists on the paper suggested that their method of removing the nucleus might explain their success. The method used was |
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Definition
| D. nicking a small hole in the egg membrane and gently squeezing out the genetic material. |
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Term
| 6. The team wanted to rule out parthenogenesis as a developmental mechanism. (Pathenogenesis occurs when an unfertilized egg begins to develop on its own.) They used a VNTR assay. If the egg developed from a diploid somatic cell, then the team expected to see __________ band(s) on a DNA gel after PCR showing that the cells were heterozygous for certain alleles. |
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Definition
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Term
| 7. To rule out parthenogenesis, the team looked at the expression of several imprinted genes in the ES cell line. (Imprinted genes are differentially expressed and show a different phenotype depending on which parent transmits a particular allele.) After RT-PCR, they found that the donor's maternal and paternal alleles were both expressed. If parthenogenesis had occurred |
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Definition
| D. only one allele would be expressed rather than two. |
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Term
A growth factor that is a member of a family of related peptides that both stimulate and inhibit cell division.
A. Transferrin
B. α1-antiplasmin
C. TGF-β
D. Fibronectin |
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Definition
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