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Tissue Culture Terms
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27
Plant Sciences
10th Grade
02/04/2010

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Term
Agar
Definition
Natural gelling agent made form algae
Term
Aseptic
Definition
Free of microorganisms
Term
Aseptic or Sterile Technique
Definition
Produres used to prevent the introduction of microorganisms into cultures
Term
Autoclave
Definition
A machine capable of sterilizing by steam under pressure
Term
Callus
Definition
A mass of wound tissue
Term
Clone
Definition
Plants produced asexually and are genetically identical
Term
Contamination
Definition
Infected by unwanted microorganisms
Term
Culture
Definition
A plant growing in vitro in a sterile environment
Term
Differentiated
Definition
Specialized palnt tissue cells
Term
Explant
Definition
Palnt parts being used to grow in tissue culture
Term
Laminar Flow Hood
Definition
An enclosed work area where the air is cleaned using HEPA filters
Term
Hormone
Definition
Generally naturally occurring chemiclas that strongly affect plant growth
Term
In Vitro
Definition
To be growen in glass
Term
In vivo
Definition
to be grown naturally
Term
Media
Definition
Plural of medium
Term
medium
Definition
a solid or liqued nutritive solution used for culturing cells
Term
micropropagation
Definition
using tissue culture to multiply plant
Term
pathogen
Definition
a disease causing organism
Term
plant tissue culture
Definition
the growth or maintenance of plant parts in vitro
Term
shoot apical meristem
Definition
undifferentiated growth tissue in the shoot tip
Term
stage 1
Definition
initiation of a culture form natural plant parts
Term
stage 2
Definition
in vitro multiplication of cultures
Term
stage 3
Definition
transferring cultures in soil in unsterial environment
Term
sterile
Definition
a cultuer that free of microoganisms
Term
subculture
Definition
explants are first subdivided then transferred to new medium
Term
totipotecy
Definition
characteristic of plants where any cell has the ability to generate new plants
Term
undifferentiated
Definition
cells that have not transformed into specialized tissues
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