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The microbiome L1
lecture 1: microbiome
16
Microbiology
Undergraduate 2
01/10/2019

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Term
Amensalism
Definition
organsim inflicting harm on another with no benefit to itself
Term
parasitism
Definition
non-mutual, one benefits at cost of the other
Term
neutralism
Definition
interaction with no effect on each other
Term
commensalism
Definition
one organism benefits, other neither benefits nor is harmed
Term
mutualism
Definition
all derive a mutual benefit
Term
the microbiome
Definition
a community of microorganisms that inhabit a particular environment
Term
Human microbiome
Definition
many roles in health and disease
produces benefiicial metabolites
mouth, skin gut
as you move down the GI, pH increases
stomach has low pH to prevent growth
large intestine is neutral w loads of growth
balance between feremcutes and bacterioids is essential
Term
prebiotics
Definition
help bacteria grow
Term
probiotics
Definition
good bacteria
Term
Gut microbiome
Definition
largest number of bacteria and species
synthesizes essential aa and vitamins
produces enzymes
ferments saccharides
provides defence against pathogens
protects against allergies, autoimmune and asthma
Term
oral microbiome
Definition
barrier against pathogens
tooth decay caused by strep
bacteria growing on teeth form biofilm called plaque
Term
skin microbiome
Definition
barrier against pathogens
hygiene paradox
some viruses/bacteria can get through sweat glands/pores
distribution of species determined by environment/lifestyle
Term
benefits for health
Definition
production of vit b and k
production of enzymes - break down of polysaccharides for easier cell uptake
carb fermentation + fatty acid production to help absorb magnesium, calcium and phosphorus
Term
problems
Definition
some species linked to disease development
dysbiosis - disturbance of balance - linked to disease, oppurtunistic pathogens grow when normal flora is destroyed
Term
study method
Definition
isolation of faecal sample
wash with PBS
vortex to remove slimy layers
DNA extraction
PCR with rRNA primers
gel electrophoresis
band purification
sequencing
compare with variety of sequences on database to match to organism
Term
Gram neg vs Gram pos
Definition
gram pos don't have outer cell membrane
gram pos have thick peptidoglycan layer
gram pos hold in gram stain
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