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Test 3 - Chapters 7,8,9
Micro Test
383
Microbiology
Undergraduate 2
11/06/2017

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Term
Nutrition
Definition
process by which chemical substances (nutrients) are acquired from the environment and used in cellular activities
Term
Essential nutrients
Definition
must be provided to an organism
Two categories of essential nutrients:
Term
Macronutrients
Definition
required in large quantities; play principal roles in cell structure and metabolism

Examples: proteins, carbohydrates, lipids
Term
Micronutrients or trace elements
Definition
required in small amounts; involved in enzyme function and maintenance of protein structure

Ex: Manganese, zinc, nickel
Term
Organic nutrients
Definition
contain carbon and hydrogen atoms and are usually the products of living things

Methane (CH4), carbohydrates, lipids, proteins, and nucleic acids
Term
Inorganic nutrients
Definition
atom or molecule that contains a combination of atoms other than carbon and hydrogen

Metals and their salts (magnesium sulfate, ferric nitrate, sodium phosphate), gases (oxygen, carbon dioxide) and water
Term
Chemical Analysis of ***Cell Contents
Definition
70% water

Proteins (most prevalent organic compounds)

96% of cell is composed of 6 elements:
Carbon
Hydrogen
Oxygen
Phosphorous
Sulfur
Nitrogen
Term
96% of cell is composed of 6 elements:
Definition
Carbon
Hydrogen
Oxygen
Phosphorous
Sulfur
Nitrogen
Term
Heterotroph
Definition
must obtain carbon in an organic form made by other living organisms such as proteins, carbohydrates, lipids, and nucleic acids
Term
Autotroph
Definition
an organism that uses CO2, an inorganic gas as its carbon source

Not nutritionally dependent on other living things
Term
Nitrogen Sources
Definition
Main reservoir is nitrogen gas (N2); 79% of earth’s atmosphere is N2

Nitrogen is part of the structure of proteins, DNA, RNA and ATP – these are the primary sources of N for heterotrophs
Some bacteria and algae use inorganic N sources (nitrate = NO3-, nitrite = NO2-, or ammonia = NH3)

Some bacteria can fix atmospheric N2 into a form that is usable by living organisms..

Regardless of how N enters the cell, it must be converted to NH3 (ammonia), the only form that can be combined with carbon to synthesize amino acids and other compounds.
Term
Oxygen Sources
Definition
Major component of carbohydrates, lipids, nucleic acids, and proteins

Plays an important role in structural and enzymatic functions of cell

Component of inorganic salts (sulfates, phosphates, nitrates) and water

O2 makes up 20% of atmosphere

Essential to metabolism of many organisms
Term
Hydrogen Sources
Definition
Major element in all organic compounds and several inorganic ones (water, salts, and gases)

Gases (hydrogen sulfide = H2S, methane = CH4, and hydrogen gas = H2), all of which are both produced and used by microbes
Term
Roles of hydrogen
Definition
Maintaining pH (stands for "power/potential" of H ion concentration)

Acceptor of oxygen during aerobic cellular respiration
Term
Phosphorous (Phosphate Sources)
Definition
Main inorganic source is phosphate (PO4-3) derived from phosphoric acid (H3PO4) found in rocks and oceanic mineral deposits

Key component of nucleic acids, essential to genetics
Term
Roles of phosphate
Definition
Serves in energy transfers (ATP = adenosine triphosphate; contains 3 phosphate atoms; breakage of bond releasing third phosphate provides energy for metabolism)
Term
Sulfur Sources
Definition
Widely distributed in environment, rocks; sediments contain sulfur or compounds (including gases) containing sulfur

Essential component of some vitamins and some amino acids
Term
Roles of sulfur
Definition
Contributes to stability of proteins by forming "disulfide bonds"
Term
Formed by sulfur to stabilize proteins
Definition
disulfide bonds
Term
Potassium
Definition
essential to protein synthesis and membrane function
Term
Sodium
Definition
important to some types of cell transport
Term
Magnesium
Definition
component of chlorophyll; membrane and ribosome stabilizer
Term
Iron
Definition
component of proteins of cell respiration
Term
Essential Organic Nutrients
Definition
Organic compounds that cannot be synthesized by an organism because they lack the genetic and metabolic mechanisms to synthesize them
Term
Growth factors
Definition
must be provided as a nutrient

Examples: Essential amino acids, vitamins
Term
Main determinants of nutritional type
Definition
If classify based on Carbon source – heterotroph, autotroph

If classify based on Energy source
:
Chemotroph – gain energy from chemical compounds (such as organics like carbohydrates, or inorganic chemicals)

Phototrophs – gain energy from light (through photosynthesis); transform it into chemical energy to be used for metabolism.
Term
Chemotroph
Definition
gain energy from chemical compounds (such as organics like carbohydrates, or inorganic chemicals)
Term
Phototrophs
Definition
gain energy from light (through photosynthesis); transform it into chemical energy to be used for metabolism.
Term
heterotroph
Definition
obtains carbon from an organic form from other organisms
Term
autotroph
Definition
an organism that uses inorganic CO2 as its carbon source
Term
Chemotroph
Definition
gain energy from chemical compounds
Term
Phototrophs
Definition
gain energy from light (through photosynthesis)
Term
Photoautotrophs
Definition
get energy from light and carbon from CO2 (include all photosynthetic organisms)
Term
Chemoautotrophs
Definition
survive totally on inorganic substances, such as hydrogen gas, sulfur, or iron, which they combine with CO2 (carbon source) and H2 to produce organic molecules and energy
Term
Methanogens
Definition
a kind of archaeon chemoautotroph; produce methane gas (as a by-product) under anaerobic conditions by combining CO2 and H2
Term
Heterotrophs and Their Energy Sources
Definition
Majority are chemoheterotrophs

Aerobic respiration (uses oxygen to break down organic compounds to produce energy)
Term
Aerobic respiration
Definition
uses oxygen to break down organic compounds to produce energy
Term
Two categories of chemoheterotrophic microbes
Definition
Saprobes
Parasites
Term
Saprobes
Definition
some are free-living microorganisms that feed on organic detritus from dead organisms; fungi and certain bacteria
Term
Opportunistic pathogen
Definition
normally nonpathogenic; cause disease in an immunologically compromised host
Term
Facultative parasite
Definition
not obligate; not restricted to a host
Term
Parasites
Definition
derive nutrients from host

Pathogens

Some are obligate parasites
Term
obligate parasites
Definition
cannot live outside the host
Term
Extracellular digestion in a
saprobe:
Definition
1) Enzymes are secreted
outside the cell.

2) Enzymes break down
the nutrients (hydrolyze
the bonds)

3) Smaller molecules are
absorbed into the cell.
Term
Passive transport
Definition
does not require energy; substances exist in a gradient and move from areas of higher concentration toward areas of lower concentration

Diffusion

Osmosis – diffusion of water

Facilitated diffusion – requires a carrier protein in the cell membrane; carries hydrophilic molecules across the hydrophobic cell membranes
Term
Osmosis
Definition
diffusion of water
Term
Facilitated diffusion
Definition
requires a carrier protein in the cell membrane; carries hydrophilic molecules across the hydrophobic cell membranes
Term
Active transport
Definition
requires energy and cell membrane protein pumps; gradient independent

Active transport (pumps molecules across membrane)

Group translocation – a type of active transport in which the transported molecule is chemically altered to its useful form inside the cell, during transport

Bulk transport – endocytosis, exocytosis, pinocytosis, phagocytosis
Term
Active transport
Definition
pumps molecules across membrane
Term
Group translocation
Definition
a type of active transport in which the transported molecule is chemically altered to its useful form inside the cell, during transport
Term
Bulk transport
Definition
ndocytosis, exocytosis, pinocytosis, phagocytosis
Term
Diffusion
Definition
Net Movement of Molecules Down Their Concentration Gradient

Ex) Sugar cube in aqueous
solution
Term
hypo
Definition
solute
lower
outside
cell
Term
hyper
Definition
solute
higher
outside
cell
Term
iso
Definition
equal solute inside and outside the cell
Term
Endocytosis
Definition
substance is enclosed by a portion of cell's membrane and forms a vesicle in the cytoplasm; two types: phagocytosis (solids engulfed); pinocytosis (liquids).
Term
phagocytosis
Definition
type of endocytosis

solids engulfed
Term
pinocytosis
Definition
type of endocytosis

liquids engulfed
Term
Exocytosis
Definition
is just the opposite of endocytosis
Term
Niche
Definition
totality of adaptations organisms make to their habitat (how they deal with environmental conditions)
Term
Environmental factors
Definition
affect the functioning of metabolic enzymes, which catalyze reactions.

Factors include:
Temperature
Oxygen requirements
pH
Osmotic pressure
Barometric (atmospheric) pressure
Term
Environmental factors that affect metabolic enzymes
Definition
Temperature
Oxygen requirements
pH
Osmotic pressure
Barometric (atmospheric) pressure
Term
Minimum temperature
Definition
lowest temperature that permits a microbe’s growth and metabolism
Term
Maximum temperature
Definition
highest temperature that permits a microbe’s growth and metabolism
Term
Optimum temperature
Definition
promotes the fastest rate of growth and metabolism
Term
Psychrophiles
Definition
optimum temperature below 15oC (59oF); capable of growth at 0oC (32oF)
Term
Mesophiles
Definition
optimum temperature 20o-40oC (68o-104oF); most human pathogens
Term
Thermophiles
Definition
optimum temperature greater than 45oC (113oF)
Term
Oxygen
Definition
As oxygen is utilized it can be transformed into several toxic products that can damage or destroy cells:

Singlet oxygen (1O2) (higher-energy species of molecular O2), superoxide ion (O2-), peroxide (H2O2), and hydroxyl radicals (OH-)

Most cells have developed enzymes that neutralize these chemicals:

Ex: superoxide ion requires 2 enzymes: superoxide dismutase and catalase, to convert the form to a nontoxic chemical form
If a microbe does not have the enzymes required to convert toxic oxygen, it is forced to live in oxygen-free habitats
Term
Aerobe
Definition
utilizes oxygen and can detoxify it
Term
Obligate aerobe
Definition
cannot grow without oxygen
Term
Facultative anaerobe
Definition
utilizes oxygen when present, but can also grow in its absence
Term
Microaerophilic
Definition
requires only a small amount of oxygen
Term
Anaerobe
Definition
does not utilize oxygen
Term
Obligate anaerobe
Definition
lacks the enzymes to detoxify oxygen so cannot survive in an oxygen environment
Term
Aerotolerant anaerobes
Definition
do not utilize oxygen but can survive and grow in its presence
Term
Categories of Oxygen Requirement
Definition
Aerobe – utilizes oxygen and can detoxify it

Obligate aerobe – cannot grow without oxygen

Facultative anaerobe – utilizes oxygen when present, but can also grow in its absence

Microaerophilic – requires only a small amount of oxygen

Anaerobe – does not utilize oxygen

Obligate anaerobe – lacks the enzymes to detoxify oxygen so cannot survive in an oxygen environment

Aerotolerant anaerobes – do not utilize oxygen but can survive and grow in its presence
Term
Carbon Dioxide Requirement
Definition
All microbes require some carbon dioxide in their metabolism

Capnophile – grows best at higher CO2 concentrations than normally present in the atmosphere
Term
Capnophile
Definition
grows best at higher CO2 concentrations than normally present in the atmosphere
Term
Effects of pH
Definition
Majority of microorganisms grow at a pH between 6 and 8

Acidophiles – grow at extreme acid pH

Alkalinophiles – grow at extreme alkaline pH
Term
Acidophiles
Definition
grow at extreme acid pH
Term
Alkalinophiles
Definition
grow at extreme alkaline pH
Term
Osmotic Pressure
Definition
Most microbes exist under slight hypotonic conditions (because their rigid cell walls withstand bursting), or isotonic conditions

Halophiles [hay’-loh] – require a high concentration of salt (hypertonic solution); have significant modifications in their cell walls and membranes.

Osmotolerant – do not require high concentration of solute such as salt but can tolerate it when it occurs.

Thus salt does not prevent all spoilage agents from growing on food, because many are osmotolerant or halophilic.
Term
Halophiles
Definition
require a high concentration of salt (hypertonic solution); have significant modifications in their cell walls and membranes.
Term
Osmotolerant
Definition
do not require high concentration of solute such as salt but can tolerate it when it occurs.
Term
Barophiles
Definition
can survive under extreme pressure and will rupture if exposed to normal atmospheric pressure (deep-sea microbes)
Term
Symbiotic
Definition
two organisms live together in a close partnership
Term
Mutualism
Definition
obligatory, dependent; both members benefit

Ex: bacteria in the gut that produce vitamins
Term
Commensalism
Definition
commensal member benefits, other member neither harmed nor benefited

Ex: flatworms attached to gills of crab; eat food particles
Term
Parasitism
Definition
parasite is dependent and benefits; host is harmed
Term
Ecological Associations Among Microorganisms
Definition
Symbiotic – two organisms live together in a close partnership

Mutualism – obligatory, dependent; both members benefit
Ex: bacteria in the gut that produce vitamins

Commensalism – commensal member benefits, other member neither harmed nor benefited
Ex: flatworms attached to gills of crab; eat food particles

Parasitism – parasite is dependent and benefits; host is harmed

Synergism – members cooperate to produce a beneficial result that none of them could do alone (Ex: one breaks down cellulose, another uses the cellulose to fix N, both use the N)

Antagonism – actions of one organism affect the success or survival of others in the same community (competition)

Antibiosis = producing chemicals that destroy other microbes nearby
Term
Synergism
Definition
members cooperate to produce a beneficial result that none of them could do alone (Ex: one breaks down cellulose, another uses the cellulose to fix N, both use the N)
Term
Antagonism
Definition
actions of one organism affect the success or survival of others in the same community (competition)
Term
Antibiosis
Definition
producing chemicals that destroy other microbes nearby
Term
normal microbial flora
Definition
Human body is a rich habitat for symbiotic bacteria, fungi, and a few protozoa
Term
Interrelationships Between Microbes and Humans
Definition
Human body is a rich habitat for symbiotic bacteria, fungi, and a few protozoa - normal microbial flora

Commensal, parasitic, and synergistic relationships exist
Term
Two microbial growth levels
Definition
growth at a cellular level with increase in size

increase in population
Term
binary fission
Definition
Division of bacterial cells occurs mainly through

means one cell becomes two
Term
transverse fission
Definition
Parent cell enlarges, duplicates its chromosome, and forms a central transverse septum dividing the cell into two daughter cells
Term
transverse
Definition
division plane forming across the width of the cell
Term
Rate of Population Growth
Definition
Time required for a complete fission cycle is called the generation, or doubling time

Each new fission cycle increases the population by a factor of 2 – exponential growth

Generation times vary from minutes to days

Equation for calculating population size over time:
Nƒ = (Ni)2n

Nƒ is the final total number of cells in the population

Ni is starting number of cells

Exponent n denotes generation time (time elapsed divided by the time it takes in minutes or hours for a generation to double)

2n = total number of cells generated in that generation time
Term
generation, or doubling time
Definition
Time required for a complete fission cycle is called
Term
exponential growth
Definition
Each new fission cycle increases the population by a factor of 2
Term
Definition
is the final total number of cells in the population
Term
Ni
Definition
is starting number of cells
Term
Exponent n
Definition
denotes generation time (time elapsed divided by the time it takes in minutes or hours for a generation to double)
Term
2n
Definition
total number of cells generated in that generation time
Term
growth curve
Definition
In laboratory studies, populations typically display a predictable pattern over time
Term
Lag phase
Definition
“flat” period of adjustment, enlargement; little growth
Term
Exponential growth phase
Definition
a period of maximum growth will continue as long as cells have adequate nutrients and a favorable environment
Term
Stationary phase
Definition
rate of cell growth equals rate of cell death caused by depleted nutrients and O2, excretion of organic acids and pollutants
Term
Death phase
Definition
as limiting factors intensify, cells die exponentially
Term
Turbidometry
Definition
simply observe growth: degree of cloudiness, "turbidity", reflects the relative population size or growth in a test tube
Term
turbidity
Definition
degree of cloudiness
Term
Turbidity measurements using a spectrophotometer
Definition
1) no growth, light passes thru
2) growth, cells scatter light
Term
Viable colony count
Definition
spreading samples on plates, then counting the colonies that grow; counts only live cells
Term
Direct cell count
Definition
count all cells present; uses a calibrated microscope slide
Term
Automated Coulter counter
Definition
uses an Electronic sensor detects each cell as it passes thru; count all cells present
Term
Enumeration of bacteria
Definition
Viable colony count – spreading samples on plates, then counting the colonies that grow; counts only live cells

Direct cell count – count all cells present; uses a calibrated microscope slide

Automated Coulter counter – uses an Electronic sensor detects each cell as it passes thru; count all cells present
Term
Metabolism
Definition
all chemical and physical workings of a cell
Term
Catabolism
Definition
degradative; breaks the bonds of larger molecules forming smaller molecules; releases energy
Term
Anabolism
Definition
biosynthesis; process that forms larger macromolecules from smaller molecules; requires energy input
Term
Enzymes
Definition
are specific proteins that serve as biological catalysts, which are molecules that increase the rate of a chemical reaction by lowering the energy of activation.

Most metabolic reactions would not occur fast enough to sustain life without the help of ...

promotes a reaction by serving as a physical site for specific substrate molecules to position for a reaction to take place.

operate under the specific temperature, pH, and osmotic pressure of organism’s habitat
Term
energy of activation
Definition
the resistance to a reaction, which must be overcome for a reaction to proceed.

The enzyme is not permanently altered in the reaction and is re-used for more reactions.
Term
substrate
Definition
a reactant molecule that participates in the reaction
Term
Simple enzymes
Definition
consist of protein alone
Term
Conjugated enzymes (also called holoenzymes)
Definition
contain a protein portion (called apoenzyme) + a nonprotein molecule (called cofactor)
Term
apoenzyme
Definition
a protein portion in Conjugated enzymes
Term
Cofactors
Definition
a nonprotein molecule in Conjugated enzymes

required by holoenzymes to be functional; can include:

Metal ions = inorganic cofactors = iron, copper, magnesium

Coenzymes = organic molecules; vitamins are common components of coenzymes
Term
Metal ions
Definition
inorganic cofactors = iron, copper, magnesium
Term
Coenzymes
Definition
organic molecules; vitamins are common components of coenzymes
Term
active site, or catalytic site
Definition
site for substrate binding on the enzyme
Term
A temporary enzyme-substrate union
Definition
occurs when substrate is helped into the active site by the enzyme making slight changes in its shape, thus creating an “induced fit”
Term
induced fit
Definition
when substrate is helped into the active site by the enzyme making slight changes in its shape, thus creating a
Term
Exoenzymes
Definition
transported extracellularly, where they break down large food molecules or harmful chemicals

Cellulase, amylase, penicillinase
Term
Endoenzymes
Definition
retained intracellularly and function there

Most enzymes are ...
Term
Location and Regularity of Enzyme Action
Definition
Exoenzymes – transported extracellularly, where they break down large food molecules or harmful chemicals
Cellulase, amylase, penicillinase

Endoenzymes – retained intracellularly and function there
Most enzymes are endoenzymes

Constitutive enzymes – always present, always produced in equal amounts or at equal rates, regardless of amount of substrate present
Enzymes involved in glucose metabolism

Regulated enzymes – not constantly present; production is turned on (induced) or turned off (repressed) in response to changes in concentration of the substrate
Term
Constitutive enzymes
Definition
always present, always produced in equal amounts or at equal rates, regardless of amount of substrate present

Enzymes involved in glucose metabolism
Term
Regulated enzymes
Definition
not constantly present; production is turned on (induced) or turned off (repressed) in response to changes in concentration of the substrate
Term
Synthesis or condensation reactions
Definition
anabolic reactions to form shared-electron bonds between smaller substrate molecules, require ATP, release one molecule of water for each bond formed
Term
Hydrolysis reactions
Definition
catabolic reactions that break down substrates into small molecules; requires the input of water to break bonds
Term
Labile
Definition
chemically unstable enzymes
Term
Denaturation
Definition
weak bonds that maintain the shape of the enzyme are broken
Term
Competitive inhibition
Definition
substance that resembles normal substrate competes with substrate for active site.
Term
Noncompetitive inhibition
Definition
enzymes are regulated by the binding of molecules (other than the substrate) on a separate site (other than the active site), which changes the shape of the enzyme and hence the active site.

Often the product molecule from the reaction catalyzed by the enzyme acts as the inhibitor molecule, thus regulating the amount of product produced by a negative feedback mechanism.

Enzyme activity can be inhibited also at the genetic level by controlling their synthesis, i.e., the products of the reactions can bind to specific sites of DNA and prevent the transcription of the genes that code for enzyme production.
Term
Biochemical and Metabolic pathways
Definition
Reactions seldom produce a product in one single reaction or step.

Usually, they occur in a series of reactions or steps, in which each step requires a specific enzyme.

Pathways can be shut down by negative feedback inhibition, which involves the accumulation of the final product participating in noncompetitive inhibition with the initial enzyme in the pathway.
Term
Energy
Definition
the capacity to do work

can be stored in the chemical bonds between atoms within molecules, to be used later when needed in metabolic reactions.

When the bonds are broken, it is released
Term
Common forms of energy
Definition
heat, light, sound, electrical energy, mechanical energy, and chemical energy.
Term
Electrons
Definition
can absorb, carry and release energy
Term
Release of Chemical Energy - Cellular Respiration
Definition
1. Requires oxygen and occurs through the breakdown of digested food molecules (nutrients) such as amino acids, fatty acids, glycerol, and glucose.

2. About 60% of the chemical energy in C.R. is released as heat, but about 40% is captured and "stored" for later use.

3. Energy is stored in the form of a molecule called ATP (adenosine triphosphate, a nucleotide with 3 phosphate groups instead of one).
Term
ATP Molecules
Definition
1. When oxygen is available, up to 38 molecules of ATP are produced for each molecule of glucose broken down.

2. contain three phosphates in a chain.

3. Energy to be used is stored in the last phosphate bond.

4. Energy is stored while converting ADP to ATP; when energy is released, ATP becomes ADP, ready to be regenerated into ATP again.
Term
Anaerobic Respiration
Definition
the 1st part of C.R.

1. The first part of cellular respiration may involve the splitting of 6-C glucose that occurs through a series of enzyme-catalyzed steps called glycolysis.

2. The result is two 3-C molecules of pyruvate (pyruvic acid).

3. Glycolysis occurs in the "cytosol" (cytoplasm) and does not require oxygen (anaerobic), but only generates a net of 2 ATP molecules.

4. Energy from ATP is used to start the process but there is a net gain of energy as a result.
Term
glycolysis
Definition
the splitting of 6-C glucose that occurs through a series of enzyme-catalyzed steps called
Term
anaerobic
Definition
does not require oxygen
Term
pyruvate (pyruvic acid)
Definition
The result is two 3-C molecules
Term
aerobic respiration
Definition
Oxygen is needed

occurs within the mitochondria of eukaryotes, but in the cell membrane and cytoplasm of prokaryotes

much greater net gain of ATP molecules up to 36 new ATP
Term
citric acid cycle
Definition
also called tricarboxylic acid cycle (TCA cycle), or the Krebs cycle), and the "electron transport chain" (in which oxygen accepts the hydrogens from glucose, making water; hence oxygen is required as the "electron acceptor"); each of these two series of reactions generate more ATP and release water and CO2 as end products.
Term
final products of aerobic respiration
Definition
carbon dioxide (waste product), water, and energy (ATP).
Term
carbon dioxide
Definition
waste product
Term
water, and energy
Definition
ATP
Term
Energy
Definition
the capacity to do work or to cause change
Term
Forms of energy
Definition
Thermal (heat) = from molecular motion
Radiant = visible light or other rays
Electrical = flow of electrons
Mechanical = physical change in position
Atomic = reactions in the nucleus of an atom
Chemical = present in the bonds of molecules
Term
Thermal (heat) Energy
Definition
from molecular motion
Term
Radiant Energy
Definition
visible light or other rays
Term
Electrical Energy
Definition
flow of electrons
Term
Mechanical Energy
Definition
physical change in position
Term
Atomic Energy
Definition
reactions in the nucleus of an atom
Term
Chemical Energy
Definition
present in the bonds of molecules
Term
Cell Energetics
Definition
Cells manage energy in the form of chemical reactions that make or break bonds and transfer electrons

Exergonic reactions – release energy

Endergonic reactions – consume energy

Energy present in chemical bonds of nutrients are trapped by specialized enzyme systems as the bonds of the nutrients are broken

Energy released is temporarily stored in high energy phosphate molecules. The energy of these molecules is used in endergonic cell reactions.
Term
Exergonic reactions
Definition
release energy
Term
Endergonic reactions
Definition
consume energy
Term
Hydrogen
Definition
Normally contains one proton and one electron, but can gives up its electron to other molecules, becoming a positively charged ... ion, often referred to simply as a “proton” (H+).
Can form hydrogen bonds with electronegative atoms.

(For simplication): In redox reactions, loss or gain of one normal ... is often referred to as the “loss or gain of one electron” (even though the ... is carrying a proton also)
Term
energy transfers
Definition
Electron transfers in redox reactions
Term
Redox reactions
Definition
always occur in pairs

There is an electron donor (hydrogen in aerobic respiration) and electron acceptor (oxygen in aerobic respiration) which constitute a redox pair

Process salvages electrons and their energy
Released energy can be captured to phosphorylate ADP or another compound
Term
Electron and Proton “Carriers” involved in cellular respiration
Definition
In the series of reactions involving respiration, carriers repeatedly accept and release electrons and hydrogen to facilitate the transfer of redox energy; thus, they “carry” the electrons and hydrogens from one molecule to another

Most carriers are coenzymes:

NAD (nicotinamide adenine dinucleotide), FAD, NADP, coenzyme A, and the compounds of the respiratory chain
Term
Adenosine Triphosphate: ATP
Definition
Metabolic “currency”

Three part molecule consisting of:
Adenine – a nitrogenous base
Ribose – a 5-carbon sugar
3 phosphate groups

utilization and replenishment is a constant cycle in active cells

Removal of the terminal phosphate releases energy

can be formed by three different mechanisms
Term
Adenine
Definition
a nitrogenous base
Term
Ribose
Definition
a 5-carbon sugar
Term
Substrate-level phosphorylation
Definition
transfer of phosphate group from a phosphorylated compound (substrate) directly to ADP; this is how both ATP's are generated in glycolysis, and 2 more ATP's in the Kreb Cycle.
Term
Oxidative phosphorylation
Definition
part of aerobic respiration pathway that uses electron carriers (NADH and FADH2) generated in glycolysis and the Kreb cycle to transport electrons down a chain of "redox" (reduced-oxidized) reactions (the “electron transport chain”), capturing energy in these reactions to phosphorylate ADP's, making more ATP's.
Term
Photophosphorylation
Definition
ATP is formed utilizing the energy of sunlight to drive photosynthesis
Term
Bioenergetics
Definition
study of the mechanisms of cellular energy release

Includes catabolic and anabolic reactions
Term
Primary catabolism of fuels (glucose) proceeds through a series of three coupled pathways:
Definition
1.Glycolysis

2.Keri’s cycle

3.Respiratory chain, electron transport
Term
Aerobic respiration
Definition
glycolysis, the Kreb’s cycle, respiratory chain
Term
Anaerobic respiration
Definition
glycolysis, the TCA cycle, respiratory chain; molecular oxygen is not final electron acceptor, but rather inorganic ions such as nitrates or sulfates
Term
Fermentation
Definition
glycolysis, organic compounds (like ethyl alcohol or lactic acid) are the final electron acceptors
Term
Aerobic Respiration
Definition
Series or enzyme-catalyzed reactions in which electrons are transferred from fuel molecules (glucose) to oxygen as a final electron acceptor

Glycolysis – glucose (6C) is oxidized and split into 2 molecules of pyruvic acid (3C), NADH is generated

TCA – processes pyruvic acid and generates CO2 molecules; NADH and FADH2 also generated

Electron transport chain – accepts electrons from NADH and FADH; generates energy through sequential redox reactions called oxidative phosphorylation
Term
Glycolysis
Definition
glucose (6C) is oxidized and split into 2 molecules of pyruvic acid (3C), NADH is generated
Term
TCA
Definition
processes pyruvic acid and generates CO2 molecules; NADH and FADH2 also generated
Term
Electron transport chain
Definition
accepts electrons from NADH and FADH; generates energy through sequential redox reactions called oxidative phosphorylation
Term
Electron Transport and Oxidative Phosphorylation
Definition
Final processing of electrons and hydrogen and the major generator of ATP

Chain of redox carriers that receive electrons from reduced carriers (NADH and FADH2)

ETS shuttles electrons down the chain, energy is released and subsequently captured and used by enzyme ATP synthase complexes to produce ATP – Oxidative phosphorylation
Term
Oxidative phosphorylation
Definition
when ATP synthase complexes to produce ATP
Term
Chemiosmosis
Definition
as the electron transport carriers shuttle electrons, they actively pump hydrogen ions (protons, positively charged, have lost their electrons) across the mitochondrial or cell membrane, setting up a concentration gradient of hydrogen ions called the proton motive force.
Term
proton motive force
Definition
concentration gradient of hydrogen ions set up by Chemiosmosis
Term
Terminal Step in ETS
Definition
Oxygen accepts 2 electrons from the ETS and then picks up 2 hydrogen ions from the solution to form a molecule of water. Oxygen is the final electron acceptor
Term
Anaerobic Respiration
Definition
Functions like aerobic respiration except it utilizes oxygen containing ions, rather than free oxygen, as the final electron acceptor

Nitrate (NO3-) and nitrite (NO2-)

Most obligate anaerobes use the H+ generated during glycolysis and the Kreb’s cycle to reduce some compound other than O2
Term
Fermentation
Definition
Incomplete oxidation of glucose or other carbohydrates in the absence of oxygen

Uses organic compounds as terminal electron acceptors; yields a small amount of ATP

Alcoholic fermentation includes conversion of pyruvic acid to ethyl alcohol by yeasts acting on glucose

Pyruvic acid may be converted to other types of acids "acid fermentation" (ex: lactic acid bacterial producers largely responsible for souring of milk; human muscles can also revert to lactic acid fermentation in the absence of oxygen to produce energy).

Carbon dioxide is another product of fermentation of various bacteria (ex: the bubbles in beer and champagne = CO2 from fermentation).
Term
Alcoholic fermentation
Definition
includes conversion of pyruvic acid to ethyl alcohol by yeasts acting on glucose
Term
acid fermentation
Definition
Pyruvic acid may be converted to other types of acids

lactic acid bacterial producers largely responsible for souring of milk; human muscles can also revert to lactic acid fermentation in the absence of oxygen to produce energy
Term
Carbon dioxide
Definition
product of fermentation of various bacteria (ex: the bubbles in beer and champagne = CO2 from fermentation).
Term
Photosynthesis
Definition
The ultimate source of all the chemical energy in cells comes from the sun

Occurs in 2 stages

Light-dependent – photons of light are absorbed by chlorophyll, carotenoid, or other pigments
Water split by photolysis, releasing O2 gas and provide electrons to drive photophosphorylation
Released light energy used to synthesize ATP and NADPH (NADP is the final electron acceptor)

Light-independent reaction – dark reactions – “Calvin cycle” – uses ATP to fix CO2 into another compound that is converted to glucose
Term
Light-dependent
Definition
photons of light are absorbed by chlorophyll, carotenoid, or other pigments

Water split by photolysis, releasing O2 gas and provide electrons to drive photophosphorylation

Released light energy used to synthesize ATP and NADPH (NADP is the final electron acceptor)
Term
Light-independent reaction
Definition
dark reactions – “Calvin cycle” – uses ATP to fix CO2 into another compound that is converted to glucose
Term
Genetics
Definition
the study of heredity
Term
The science of genetics
Definition
1. Transmission of biological traits from parent to offspring

2. Expression and variation of those traits

3. Structure and function of genetic material

4. How this material changes
Term
Genome
Definition
sum total of genetic material of a cell (chromosomes + mitochondria/chloroplasts and/or plasmids)
Term
DNA
Definition
Genome of all nonviral cells
Term
DNA or RNA
Definition
Genome of viruses
Term
chromosomes
Definition
DNA complexed with protein constitutes the genetic material
Term
Bacterial chromosomes
Definition
a single circular loop
Term
Eukaryotic chromosomes
Definition
multiple and linear
Term
Gene
Definition
Chromosome is subdivided into

The fundamental unit of heredity responsible for a given trait

Site on the chromosome that provides information for a certain cell function

Segment of DNA (a specific sequence of nucleotides) that contains the necessary code to make a protein or RNA molecule
Term
structural genes
Definition
Genes that code for proteins
Term
regulatory genes
Definition
Genes that control gene expression
Term
Three basic categories of genes
Definition
1. Genes that code for proteins – structural genes

2. Genes that code for RNA

3. Genes that control gene expression – regulatory genes
Term
genotype
Definition
All types of genes constitute the genetic makeup

Ex: Genes that express blue eye color.
Term
phenotype
Definition
The expression of the genotype creates observable traits

Ex: blue eyes
Term
Genomes Vary in Size
Definition
Smallest virus has 4-5 genes

E. coli – single chromosome containing 4,288 genes and is 1 mm in length

this is 1,000X longer than the E. coli cell

Human cell – 46 chromosomes containing 31,000 genes; 6 feet long; 180,000X longer than a human cell
Term
Smallest virus
Definition
has 4-5 genes
Term
E. coli
Definition
single chromosome containing 4,288 genes and is 1 mm in length

this is 1,000X longer than the E. coli cell
Term
Human cell
Definition
46 chromosomes containing 31,000 genes; 6 feet long; 180,000X longer than a human cell
Term
DNA
Definition
Two strands twisted into a double helix

Basic unit of DNA structure is a nucleotide

Each nucleotide consists of 3 parts:
A 5 carbon sugar – deoxyribose
A phosphate group
One of 4 nitrogenous bases – adenine, guanine, thymine, or cytosine

Nucleotides covalently bond (share electrons) in an arrangement that forms a sugar-phosphate linkage = the backbone

Each sugar attaches to two phosphates
Term
nucleotide
Definition
Basic unit of DNA structure
Term
3 parts of a nucleotide
Definition
A 5 carbon sugar – deoxyribose

A phosphate group

One of 4 nitrogenous bases – adenine, guanine, thymine, or cytosine
Term
covalently bond
Definition
share electrons
Term
backbone
Definition
Nucleotides covalently bond (share electrons) in an arrangement that forms a sugar-phosphate linkage
Term
DNA
Definition
Nitrogenous bases covalently bond to a carbon of each sugar and span the center of the molecule to pair with an appropriate complementary base on the other strand

Adenine always binds to thymine with 2 hydrogen bonds
Guanine always binds to cytosine with 3 hydrogen bonds

Each strand provides a template for the exact copying of a new strand

Order of bases constitutes the DNA code
Term
Adenine
Definition
always binds to thymine with 2 hydrogen bonds
Term
Guanine
Definition
always binds to cytosine with 3 hydrogen bonds
Term
RNAs
Definition
Single-stranded molecule made of nucleotides

5 carbon sugar is ribose

One of 4 nitrogenous bases – adenine, uracil (instead of thymine), guanine, or cytosine

Phosphate
Term
ribose
Definition
5 carbon sugar
Term
uracil
Definition
replaces thymine in RNA
Term
Maintenance of code during reproduction
Definition
Constancy of base pairing guarantees that the code will be retained
Term
Providing variety
Definition
differences in the order of bases responsible for variable traits and unique qualities of each organism
Term
DNA Replication
Definition
Making an exact duplicate of the DNA involves 30 different enzymes

Begins at an origin of replication

semiconservative
Term
Helicase
Definition
part of DNA Replication

(enzyme) unwinds and unzips the DNA double helix
Term
RNA primer
Definition
part of DNA Replication

synthesized at the origin of replication

short strand of RNA that serves as a starting point for adding nucleotides
Term
DNA polymerase
Definition
part of DNA Replication

adds nucleotides

Both strands are copied (synthesized)
Term
DNA polymerase
Definition
part of DNA Replication

the RNA primers and replaces them with DNA

Separation of the daughter molecules is complete
Term
semiconservative
Definition
it preserves the exact sequence of genes in the original parent molecule, because each chromosome ends up with one new strand of DNA and one old strand.
Term
messenger RNA
Definition
Information stored on the DNA molecule is conveyed to

relatively short single-stranded segments of nucleotides that code for proteins.
Term
transcription
Definition
Information stored on the DNA molecule is conveyed to "messenger RNA" molecules (mRNA) through the process of

occurs at the chromosome
Term
template strand
Definition
An mRNA is transcribed from only one strand of DNA

which contains the gene

Which strand becomes the “...” varies from one gene to another.
Term
nontemplate strand
Definition
the other complementary DNA strand
Term
RNA polymerase
Definition
binds the DNA at the starting point of the gene to be transcribed, and begins binding together nucleotides that are complementary but in the same sequence as those within the DNA template strand, forming the mRNA segment.

The mRNA segment then moves to a ribosome where the code is translated into a protein.
Term
AUG
Definition
is always the first triplet of nucleotides in the mRNA, and always codes for the first amino acid in a protein, so the first triplet in the template strand is “TAC”.)
Term
TAC
Definition
first triplet in the template strand
Term
codon
Definition
Each "triplet" of nucleotides on the mRNA

specifies a particular amino acid
Term
translation
Definition
The information contained in the mRNA molecule is used to produce proteins in a process called

which occurs in the ribosome (which is in the cytoplasm)
Term
tRNA ("transfer RNA")
Definition
bring the proper amino acids to the ribosomes, in the sequence encoded in the mRNA.

There exist a specific ... molecule for each kind of amino acid.

Each ... carries an anticodon
Term
anticodon
Definition
which is a triplet of nucleotides that binds to a complementary codon in the mRNA. Hence the amino acids are brought to the ribosomes in the proper order, and then bonded together in the proper sequence.
Term
From DNA to Protein
Definition
1. A protein’s primary structure determines its shape and function

2. Proteins determine phenotype. Living things are largely what their proteins make them.

3. DNA is mainly a blueprint that tells the cell which kinds of proteins to make and how to make them
Term
The Master Genetic Code
Definition
Represented by the mRNA codons and the amino acids they specify

Code is universal

Code is redundant (repetitious)
Term
Genetics of Animal Viruses
Definition
Viral genome - one or more pieces of DNA or RNA; contains only genes needed for production of new viruses

Requires access to host cell’s genetics and metabolic machinery to instruct the host cell to synthesize new viral particles
Term
Viral genome
Definition
one or more pieces of DNA or RNA; contains only genes needed for production of new virus
Term
Regulation of Protein Synthesis and Metabolism
Definition
Genes are regulated to be active only when their products are required

In prokaryotes this regulation is coordinated by operons = a set of genes, all of which are regulated as a single unit
Term
operons
Definition
a set of genes, all of which are regulated as a single unit
Term
2 types of Operons
Definition
1) Inducible – operon is turned ON by the presence of the substrate:

Catabolic operon - a set of structural genes that code for the synthesis of an enzyme needed to metabolize (break down, catabolize) a nutrient; this inducible operon is "induced" when the substrate is present;

Ex: if the substrate lactose (a sugar) is present, bacteria induces the operon that codes for the synthesis of lactase; lactase catalyzes the breakdown of lactose.

Substrate = the reactant molecule(s) that an enzyme binds to, causing a reaction and forming products

2) Repressible – genes in a series are turned OFF by the product synthesized

Anabolic operon – a set of structural genes that code for the synthesis of an enzyme used to synthesize (anabolism) products, such as an amino acid; this repressible operon is "repressed" by the product of which the enzyme catalyzes the production of.
Term
Inducible
Definition
operon is turned ON by the presence of the substrate
Term
Catabolic operon
Definition
a set of structural genes that code for the synthesis of an enzyme needed to metabolize (break down, catabolize) a nutrient; this inducible operon is "induced" when the substrate is present;

Ex: if the substrate lactose (a sugar) is present, bacteria induces the operon that codes for the synthesis of lactase; lactase catalyzes the breakdown of lactose.
Term
Substrate
Definition
the reactant molecule(s) that an enzyme binds to, causing a reaction and forming products
Term
Repressible
Definition
genes in a series are turned OFF by the product synthesized
Term
Anabolic operon
Definition
a set of structural genes that code for the synthesis of an enzyme used to synthesize (anabolism) products, such as an amino acid; this repressible operon is "repressed" by the product of which the enzyme catalyzes the production of.
Term
Lactose Operon: an Inducible Operon
Definition
Made of 3 genes each coding for an enzyme needed to catabolize lactose –

b-galactosidase – hydrolyzes lactose

permease – brings lactose across cell membrane

b-galactosidase transacetylase – uncertain function
Term
b-galactosidase
Definition
hydrolyzes lactose
Term
permease
Definition
brings lactose across cell membrane
Term
b-galactosidase transacetylase
Definition
uncertain function
Term
Lac Operon
Definition
Normally off
In the absence of lactose, transcription is blocked so that genes encoding the enzymes are not active

Lactose turns the operon on
Binding of lactose to proteins covering the DNA code turns the genes "on" and genes are transcribed.
Term
Normally off
Definition
In the absence of lactose, transcription is blocked so that genes encoding the enzymes are not active
Term
Lactose turns the operon on
Definition
Binding of lactose to proteins covering the DNA code turns the genes "on" and genes are transcribed.
Term
mutation
Definition
A change in phenotype due to a change in genotype (nitrogen base sequence of DNA) is called a

results from a deleted, added, or substituted nucleotide, which changes the sequence and thus the code
Term
wild type (wild strain)
Definition
The natural, non mutated characteristic in a genetic code is known as the
Term
mutant strain
Definition
An organism that has a mutation is called a
Term
A mutation can result in
Definition
a variance in morphology, nutritional characteristics, genetic control mechanisms, resistance to chemicals, etc.
Term
Causes of Mutations
Definition
Spontaneous mutations – random change in the DNA due to errors in replication that occur without known cause

Induced mutations – result from exposure to known mutagens, which are physical (primarily radiation) or chemical agents that interact with DNA in a disruptive manner
Term
Spontaneous mutations
Definition
random change in the DNA due to errors in replication that occur without known cause
Term
Induced mutations
Definition
result from exposure to known mutagens, which are physical (primarily radiation) or chemical agents that interact with DNA in a disruptive manner
Term
mutagens
Definition
which are physical (primarily radiation) or chemical agents that interact with DNA in a disruptive manner
Term
Repair of Mutations
Definition
Since mutations can be potentially fatal, the cell has several enzymatic repair mechanisms in place to find and repair damaged DNA

DNA polymerase – proofreads nucleotides during DNA replication
Term
DNA polymerase
Definition
proofreads nucleotides during DNA replication
Term
Positive and Negative Effects of Mutations
Definition
Mutations leading to nonfunctional proteins are harmful, possibly fatal.

Organisms with mutations that are beneficial in their environment can readily adapt, survive, and reproduce – these mutations are the basis of changes in populations (evolution).

Any change in genes that confers an advantage during selection pressure (the environment selecting one feature over another in favor of survival and enhanced reproduction) will be retained by the population, and passed on to offspring.
Term
selection pressure
Definition
the environment selecting one feature over another in favor of survival and enhanced reproduction
Term
Genetic recombination
Definition
occurs when an organism acquires and expresses genes that originated in another organism
Term
3 means for genetic recombination in bacteria:
Definition
1. Conjugation

2. Transformation

3. Transduction
Term
Conjugation
Definition
transfer of a plasmid or chromosomal fragment (DNA) from a donor cell to a recipient cell via a direct connection (plus)

Example:
Gram-negative cells have a fertility factor, which is a plasmid that contains genes that code for the synthesis of a ... plus

Recipient cell is a related species or genus without a fertility factor

Donor replicates its plasmid and then transfers fertility factor to recipient through its pilus; now recipient can synthesize pili, thus acquiring a new ability to initiate "sex"
Term
fertility factor
Definition
which is a plasmid that contains genes that code for the synthesis of a conjugative pilus
Term
Some other traits acquired by conjugating bacteria
Definition
Drug or antibiotic resistance (acquired first by random mutation in the DNA that gives bacteria a selective advantage in its environment)

plasmids contain the genes that give the bacteria this type of resistance

New enzymes

Toxin production

Metal resistance
Term
Transformation
Definition
chromosome fragments from a lysed cell are accepted by a recipient cell that has special DNA-binding proteins on its cell wall; the genetic code of the DNA fragment is acquired by the recipient and inserted into its own chromosome

Donor and recipient cells can be unrelated

Useful tool in recombinant DNA technology

genes from humans and other organisms can be inserted into a plasmid and then introduced into bacteria through transformation; as the bacteria reproduces, it replicates its entire genome, including the new plasmid, before dividing into two new daughter cells. Using this recombination technique, many copies of a gene can be made.
Term
Transduction
Definition
bacteriophage serves as a carrier of new genes by acquiring random fragments of the host cell's DNA during assembly of new viruses within the host cell

The bacteriophage produces an enzyme that breaks up the host cell DNA

During assembly of new viruses, some of the viral assemblies may take up one of the host cell's DNA fragments instead of the viral DNA or RNA

Upon infecting a new host bacterial cell, these new genes can be inserted into the recipient cell's genome
Term
Transduction
Definition
This process utilizes bacteriophages to carry DNA from a donor cell to a recipeint cell
Term
Transcription
Definition
This process makes an RNA copy of a DNA strand
Term
Before
Definition
The above process happens Before or After translation
Term
Amino acid sequences that become PROTEINS
Definition
In translation, the RNA copy is then "translated" into what?
Term
Replication
Definition
The dublication of a cell's DNA is
Term
Adenine - Thymine (in DNA)

Cytosine - Guanine (in both DNA & RNA)

Adenine - Uracil (in RNA)
Definition
List the nitrogenous bases and what they pair to
Term
Transfer RNA
Definition
The RNA that carries amino acids to the ribosome is
Term
RNA
Definition
This molecule contains ribose, uracil and is typically single stranded
Term
Metabolism
Definition
All of the chemical reactions within a cell are?
Term
Catabolism
Definition
A metabolic process that breaks down compounds is?
Term
Anabolism
Definition
A metabolic process that builds new compounds is?
Term
Enzymes
Definition
The proteins that act as catalyst?
Term
Coenzymes
Definition
Vitamins are often important components of?
Term
Induced enzymes
Definition
Enzymes ONLY present when a substrate is present?
Term
Phosphorylation
Definition
The process of using energy to convert ADP to ATP is?
Term
Exo - secreted outside of the cell

Endo - utilized within the cell (most are this)
Definition
What is the difference in an ExoEnzyme and an EndoEnzyme?
Term
Oxygen
Definition
During aerobic cellular respiration, what is the final electron acceptor?
Term
Cell Membrane
Definition
Cellular respiration occurs here in bacterial cells
Term
Electron Transport Chain
Definition
During which phase of cellular respiration is the most ATP formed?
Term
38 ATP
Definition
How many ATP are formed when one molecule of glucose is fully catabolized in the presence of oxygen?
Term
Alcohol (usually ethyl) & carbon dioxide
Definition
What are the products of alcoholic fermentation?
Term
Cytoplasm
Definition
Where does glycolysis occur in eukaryotic cells?
Term
Mitochondria
Definition
Where does the Krebs cycle & Electron transport occur in Eukaryotes?
Term
CO-2 & Water & Light energy
Definition
What are the reactants in photosynthesis?
Term
Glucose & oxygen
Definition
What are the products in photosynthesis?
Term
Macronutrients
Definition
When organisms require large amounts of a nutrient. It is?
Term
Trace Elements or Micronutrients
Definition
When organisms require small amounts of a nutrient. It is?
Term
Growth Factor
Definition
When an organism cannot synthesize a compound and therefore it must be supplied in the diet. This is called a?
Term
AUTO - produces its own food using CO-2 as their carbon source

PHOTO - produces its own food using LIGHT as their energy source
Definition
What is the difference in an AUTOTROPH and a PHOTOTROPH?
Term
Heterotroph
Definition
An organism that obtains their carbon from organic sources is called a?
Term
Saprobe
Definition
Organisms that feed on dead and decaying material for their nutrients are?
Term
Diffusion
Definition
Movement from high concentration to low concentration (requires no energy)
Term
Osmosis
Definition
Diffusion of water through a semipermeable membrane (requires no energy)
Term
Active Transport
Definition
Movement of a substance across a membrane from low to high concentration using a carrier protein (requires energy) is?
Term
Group Translocation/Bulk Transport/Active Transport
Definition
Endocytosis, exocytosis, pinocytosis & phagocytosis are all?
Term
Psychrophiles
Definition
Organisms that thrive at lower temperatures
Term
Thermophiles
Definition
Organisms that thrive at higher temperatures
Term
Halophiles
Definition
Organisms that thrive at higher salt concentration
Term
Barophiles
Definition
Organisms that thrive at higher pressure
Term
Capnophiles
Definition
Organisms that thrive at higher carbon dioxide concentration
Term
Mutualism
Definition
Organisms that cohabitate and benefit both
Term
Commensalism
Definition
Organisms that cohabitate and one is benefited;
The other is unaffected
Term
Parasitism
Definition
Organisms that cohabitate and one is benefited;
The other is harmed
Term
Generation Time
Definition
The time interval from ONE parent cell to TWO daughter cells is?
Term
Log (Exponential) Phase
Definition
Rate of cell duplication exceeds cell death
Term
Stationary Time
Definition
Rate of cell duplication is equal to the rate of cell death
Term
Death Phase
Definition
Rate of cell death exceeds duplication
Term
Transduction
Definition
This process utilizes bacteriophages to carry DNA from a donor cell to a recipeint cell
Term
Transcription
Definition
This process makes an RNA copy of a DNA strand
Term
Before
Definition
The above process happens Before or After translation
Term
Amino acid sequences that become PROTEINS
Definition
In translation, the RNA copy is then "translated" into what?
Term
Replication
Definition
The dublication of a cell's DNA is
Term
Adenine - Thymine (in DNA)

Cytosine - Guanine (in both DNA & RNA)

Adenine - Uracil (in RNA)
Definition
List the nitrogenous bases and what they pair to
Term
Transfer RNA
Definition
The RNA that carries amino acids to the ribosome is
Term
RNA
Definition
This molecule contains ribose, uracil and is typically single stranded
Term
Metabolism
Definition
All of the chemical reactions within a cell are?
Term
Catabolism
Definition
A metabolic process that breaks down compounds is?
Term
Anabolism
Definition
A metabolic process that builds new compounds is?
Term
Enzymes
Definition
The proteins that act as catalyst?
Term
Coenzymes
Definition
Vitamins are often important components of?
Term
Induced enzymes
Definition
Enzymes ONLY present when a substrate is present?
Term
Phosphorylation
Definition
The process of using energy to convert ADP to ATP is?
Term
Exo - secreted outside of the cell

Endo - utilized within the cell (most are this)
Definition
What is the difference in an ExoEnzyme and an EndoEnzyme?
Term
Oxygen
Definition
During aerobic cellular respiration, what is the final electron acceptor?
Term
Cell Membrane
Definition
Cellular respiration occurs here in bacterial cells
Term
Electron Transport Chain
Definition
During which phase of cellular respiration is the most ATP formed?
Term
38 ATP
Definition
How many ATP are formed when one molecule of glucose is fully catabolized in the presence of oxygen?
Term
Alcohol (usually ethyl) & carbon dioxide
Definition
What are the products of alcoholic fermentation?
Term
Cytoplasm
Definition
Where does glycolysis occur in eukaryotic cells?
Term
Mitochondria
Definition
Where does the Krebs cycle & Electron transport occur in Eukaryotes?
Term
CO-2 & Water & Light energy
Definition
What are the reactants in photosynthesis?
Term
Glucose & oxygen
Definition
What are the products in photosynthesis?
Term
Macronutrients
Definition
When organisms require large amounts of a nutrient. It is?
Term
Trace Elements or Micronutrients
Definition
When organisms require small amounts of a nutrient. It is?
Term
Growth Factor
Definition
When an organism cannot synthesize a compound and therefore it must be supplied in the diet. This is called a?
Term
AUTO - produces its own food using CO-2 as their carbon source

PHOTO - produces its own food using LIGHT as their energy source
Definition
What is the difference in an AUTOTROPH and a PHOTOTROPH?
Term
Heterotroph
Definition
An organism that obtains their carbon from organic sources is called a?
Term
Saprobe
Definition
Organisms that feed on dead and decaying material for their nutrients are?
Term
Diffusion
Definition
Movement from high concentration to low concentration (requires no energy)
Term
Osmosis
Definition
Diffusion of water through a semipermeable membrane (requires no energy)
Term
Active Transport
Definition
Movement of a substance across a membrane from low to high concentration using a carrier protein (requires energy) is?
Term
Group Translocation/Bulk Transport/Active Transport
Definition
Endocytosis, exocytosis, pinocytosis & phagocytosis are all?
Term
Psychrophiles
Definition
Organisms that thrive at lower temperatures
Term
Thermophiles
Definition
Organisms that thrive at higher temperatures
Term
Halophiles
Definition
Organisms that thrive at higher salt concentration
Term
Barophiles
Definition
Organisms that thrive at higher pressure
Term
Capnophiles
Definition
Organisms that thrive at higher carbon dioxide concentration
Term
Mutualism
Definition
Organisms that cohabitate and benefit both
Term
Commensalism
Definition
Organisms that cohabitate and one is benefited;
The other is unaffected
Term
Parasitism
Definition
Organisms that cohabitate and one is benefited;
The other is harmed
Term
Generation Time
Definition
The time interval from ONE parent cell to TWO daughter cells is?
Term
Log (Exponential) Phase
Definition
Rate of cell duplication exceeds cell death
Term
Stationary Time
Definition
Rate of cell duplication is equal to the rate of cell death
Term
Death Phase
Definition
Rate of cell death exceeds duplication
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