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Test 3 2011
meeks
116
Microbiology
Graduate
11/15/2011

Additional Microbiology Flashcards

 


 

Cards

Term
small pox and polio are caused from what?
Definition
virsuses
Term
Possibly the first written record of a viral infection was in1400 BC.  It was found in a Temple Priest named _________ and he shows signs of Paralytic Poliomyelitis
Definition
Siptah
Term
What viral disease do we think Pharaoh Ramses V died from?

Definition
He died in 1196 BC is believed to have succumbed to small pox. Pustular leagions were found on the face and body of the mummy.
Term
What do we call viruses that can infect bacteria?
Definition
bacteriophages
Term
who discovered bacteriophages?
Definition
Fredrick Twort and Feliz d'Herelle
Term
What does the word virus mean?
Definition
Virus is the latin word for poison
Term
what are the characteristics of a virus
Definition

Living characteristics of viruses

a. They reproduce at a fantastic rate, but only in living host cells.

b. They can mutate.

2. Nonliving characteristics of viruses

a. They are acellular, that is, they contain no cytoplasm or cellular organelles.

b. They carry out no metabolism on their own and must replicate using the host cell's metabolic machinery. In other words, viruses don't grow and divide. Instead, new viral components are synthesized and assembled within the infected host cell.

c. TThe vast majority of viruses possess either DNA or RNA but not both

Term
who worked on the panama canal episode?
Definition
Walter Reed
Term
What viral disease is associated with Walter Reed?
Definition
Yellow fever that was spread by mosquitoes
Term
How did the number of deaths from combat compare to the number of deaths from disease during early wars
Definition
mor people dies from diseases than actual combat
Term
What is another name for Yellow Fever?
Definition
yellow jacket
Term
Who discovered attenuated vaccines
Definition
Max Theiler
Term
What are attenuated vaccines
Definition
A virulent organism that has been modified to produce a less virulent form, but nevertheless retains the ability to elicit antibodies against the virulent form
Term
What nucleic acids are present in a virus?
Definition
some viruses use RNA as their genetic material and others use DNA but never do they contain both
Term
What composes the envelope on a virus?
Definition
composed of lipids, proteins and carbs
Term
Where can we find viruses
Definition
viruses are everywhere, they attack perhaps every form of cellular life on this planet
Term
Can they crystallize?
Definition
yes, they can crystallize like inorganic salts and protein molecules
Term
Even within a host viruses can attach to and invade only those cells with the _______.
Definition
appropriate receptor sites
Term
Explain the phases of the life cycle of a virus in order.
Definition
1. Absorption 2.Entry/Penetration 3. Biosynthesis 4. Assembly/Maturation 5. Release
Term
How long does the virus life cycle take?
Definition
from infection to lysis takes 20-40 minutes to produce 50-200 new viruses
Term
Which viral stage is dormant?
Which is active?
Definition

dormant: Viron stage

active: infectious stage

Term
What are other names for the lysogenic pathway?
Definition
also known as temperate or LATENT
Term
 an infectious agent that is composed primarily of protein
Definition
Prions
Term
Name diseases caused by prions
Definition

Scrapies (sheep)    

Mad Cow Disease (cows)

         Kuru (fore tribe in new guiena) 

Creutzfeldt-jakob disease (humans)

 

Term
What nucleic acid is present in a prion?
Definition
no nucleic acid, all protein
Term
What are the symptoms of scrapi?

What animal is involved?
Definition

it affects sheep

 

symptoms: intense itching, scrap off wool

Term
does heating meat kill prions?
Definition
no, if infected all animals in that place has to die including the ones around it
Term
What does BSE stand for?

What animal is involved?

What are the symptoms
Definition

Mad Cow Disease

 

cows

 1. the first symptoms are the the cow  has a change in attitide and then cant perform certain tasks such as walking then lose appetite and cannot produce milk.

Term

 

Presented a paper to the St. Petersburg Academy of Science
Showed that extracts from diseased tobacco plants could transmit disease to other plants after passage through ceramic filters.
Definition
Dmitri Iwanowski
Term

 

Animal viruses were soon discovered via the isolation of the virus responsible for a disease of cattle, foot and mouth disease, in 1898.

Definition
FreidrichLoeffler and
Paul
Frosch
Term
First human disease
Definition
Polio
Term
The discovery of the first human virus (poliomyelitis) followed in 1900 with the isolation of the yellow fever virus
Definition
Landsteiner and Popper
Term
what are the easiest viruses to grow?
Definition
bacteriophages
Term
What is the case fatality rate for prion diseases?
Definition
100% fatal
Term
What is a viroid? 
What organisms do
viroids infect
Definition

 composed of naked RNA that are only 300-400 nucleotides long

 

they affect plants only

Term
What are virusoids?
What do
virusoids infect?
Definition
virusoids kill viruses
Term
Name some viral diseases
Definition
Influenza, polio, measles, chickenpox, common cold, herpes
Term
Name some oncongenic viruses.
Definition
human leukemia, lymphoma, epstein barr disease, hodgkins and kaposi sarcoma
Term
Name some diseases caused by viruses that have no vaccine.
Definition
common cold, herpes
Term
What are the two ways of controlling microbial growth?
Definition
physical and chemical methods
Term
physical methods are..
Definition
heating up, filtration, low temperatures, desiccation, osmotic pressure and radiation
Term
chemical methods are..
Definition
disinfectants, antiseptics
Term
Regarding level of resistance, what is the most difficult thing to destroy?
Definition
endospores
Term
what is the easiest microorgamism to destroy?
Definition
bacteria
Term
the control of microbial growth can prevent infections and food spoilage
Definition
prevention
Term
process of destroying all microbial life on an object
Definition
sterilization (complete distructing)
Term
heat treatment of canned foods to destroy botilinum endospores
Definition
commerical sterilization
Term
the process of reducing or inhibiting microbial gorwth on a NONliving surface
Definition
disinfection
Term
process of reducing or inhibiting microorganisms on LIVING tisses
Definition

antisepsis

 

example: hydrogen peroxide on tissue

Term
means to swab the skin
Definition

degerming

 

ex. before surgery

Term
means to subject to high temperatues washing
Definition
sanitize
Term
suffix ___ means to kill
Definition

--cide

 

ex. insectiCIDE - to kill insects

Term
suffix ___ means to inhibit
Definition
---stat
Term
bacterial contamination...
Definition
sepsis
Term
lack of bacterial contamination
Definition
asepsis
Term
HEPA filters are used in..
Definition

fume hoods

 burn units

operating rooms

Term
disrupts microbial metabolism, buy extracting water
Definition
desiccation
Term
lowest temperature at which all the bacteria in a liquid culture will be killed in 10 minutes
Definition
(TDP) theraml death point
Term
the length of time required to kill all bacteria in a liquid culture at a given temperature
Definition
(TDT) thermal death time
Term
length of time in which 90% of a bacterial population will be killed at a given temp
Definition
(DRT) decimal reduction time
Term
high temps are used for a short time to destroy pathogens without altering the flavor of the food
Definition
pasteurization
Term
The time to kill is proportion to the________.
Definition
to the number of microbes
Term
At what rate to bacteria die?
Definition
death rate as a straight line (constant rate)
Term
Why does organic matter interfere with our disinfecting and antiseptic techniques?
Definition

vomit

feces

blood

Term
How long do I need to boil something?
Definition
10 minutes but if you want to be safe, 30 minutes
Term
when Autoclaving.  How long?  At what temperature?  At what pressure
Definition

15 minutes,

at 121 degrees C 

at 15lbs per square inch

Term
Who discovered Pasteurization?
Definition
luis pasteur
Term
What things do we pasteurize?
Definition
milk, wine, beer
Term
Is pasteurization sterilization?
Definition
NO!!
Term
Why might ultra high heat sterilization be preferred over pasteurization?
Definition
because it is just a higher temperature but is done more quickly
Term
When do we use filtration?
Definition
when we are trying to filter things that are in the air
Term
How are low temperatures beneficial?
Definition
it prevents them from growing, does not kill, decreases chemical activites of the microorganisms
Term
Give examples of desiccation.
Definition
freeze-drying or dehydrating
Term
Does desiccation affect viruses and endospores?
Definition
Viruses and endospores CAN resist desiccation
Term
Even after salt/sugar curing meat we may still get some growth of _______.
Definition

molds and yeasts more than bacteria.

 

Plasmolisis

Term
How does radiation kill?
Definition
normally destroys DNA or damages it
Term
What is radiation efficiency dependent on?
Definition
wavelength, intensity and duration
Term
What is the most lethal type of radiation?
Definition
ultraviolet radiation
Term
Unfortunately ultraviolet radiation does not _______.
Definition
penetrate glass, plastic, or water very well
Term
Give examples of ionizing radiation
Definition

gamma rays

x-rays

high energy electron beams

Term
Why do we use gamma radiation?
Definition
it ecerts its effect primarily by ionizing water and forming highly reactive hydroxyl radicals
Term
How well does ionizing radiation penetrate?
Definition
high degree of pentration
Term
How do microwaves kill?
Definition
kill microbes indirectly as material get hot
Term

used in lysol

last a long time

good for bloody and body fluid clean up

can be harmful to house animals

Definition
Phenol and Phenolics
Term

damages plasma membranes in vvegetative cells

can also danage eyes when splashed

used before surgical procedures

Definition
biguanides
Term

ex. iodine and chlorine

do not mix with other cleaners

kills germs by forminf an acid when added to water

Definition
halogens
Term

exert action by denaturing proteins and dissolving lipids

 

aqueous ethanol and isopropanol are used as wet disinfectants

Definition
alcohols
Term

often used for burn treatment

 

they denature proteins

 

ex. silver, mercury, copper

Definition
heavy metals and their compounds
Term

decrease the tention between molecules that lie on the surface of a liquid

ex. soaps and detergents

 

Definition
surface-active agents
Term

mouthwash and sore throat remedies

 

disrupt plasma membranes and allow cytoplasmic constituents to leak out of cell

 

cationic detergents attached to ammonium NH+4

Definition
quaternary ammonium compounds
Term

ex. sorbic acid, benzoic acid, propionic acid

 

inhibit fungal matabolism and are used as food preseratives

 

 

Definition
chemical food preservatives
Term

extremely affective

 

AKA carcinogens

 

exert their effect by oxidizing molecules inside cells

Definition
aldehydes
Term

nisin and natamycin are used to preserve foods, especially cheese

 

this resistance is becoming a big problem

Definition
antibiotics
Term
Phenol and Phenolics are another name for what..
Definition
carbolic acid
Term
Phenols and Phenolics exert influence by _____.
Definition

injuring plasma membrane

inactivating enzymes

denaturing protein

Term
How does iodine work?
Definition
iodine combines with amino acids to inactivate enzymes
Term
When do we use heavy metals?

Definition
often used for burn treatment and prevent neonatal gonorrheal opthalmia
Term
What are QUATS most effective on?
Definition
most affective againt gram-postitve bacteria
Term
What are the super bugs
Definition

they are the viruses that are antibiotice resistant

 

Staph aureus

enterococcus

mycobacterium tuberculosis

Term
Barbarossa put human corpses in his enemy’s water supply, successfully contaminating it
Definition
Battle of Tortona, Italy 1155
Term

 

Czech Republic – Bodies of plague-stricken soldiers plus 2000 cartloads of excrement are hurled into the ranks of enemy troops causing deadly fevers to quickly break out.
Definition
Battle of Carolstein 1422
Term

 

The Spanish supplied their French enemies with wine laced with leprosy patients’ blood.
Definition
1485 Naples
Term

 

Improved his chances of victory by presenting to the natives, as gifts, clothing laden with the smallpox virus.
Definition
Pizarro’s Conquest of South America 15th Century
Term

 

French and Indian War (Indians greatly outnumbered the British and were suspected of being on the side of the French)
Commander of British Forces, directs that small-pox bearing blankets be given to Indians in the Ohio River Valley.
Smallpox decimated the Indians
Definition
Sir Jeffrey Amherst
Term

 

The Austrian fortress at Mantua was of vital strategic importance to both sides during the 1796-1797 campaigns in Northern Italy.
attempted to force the surrender of Mantua by infecting the citizens with Swamp Fever.
Definition

 

Napoleon
Term

 

W.T. Sherman’s memoirs contain accounts of invading forces often slaughtering animals and dumped the rotting carcasses on water wells as they passed through enemy territory.
They obtained the idea from the Romans, who used dead animals to foul the water supply of their enemies.
Definition
US civil war
Term

W.T. Sherman’s memoirs contain accounts of invading forces often slaughtering animals and dumped the rotting carcasses on water wells as they passed through enemy territory.

They obtained the idea from the Romans, who used dead animals to foul the water supply of their enemies.
Definition
W.T. Sherman’s
Term
During the American Civil War, who would later become Kentucky’s governor, tried to infect Union troops by providing them with clothing exposed to smallpox and yellow fever
Definition
Dr. Luke Blackburn
Term

 

Anthrax is used to infect food animals and Glanders to infect horses used by the Calvary.
Definition
Germany in WWI
Term

 

Attempted to spread

Cholera in Italy
Plague in St. Petersburg
Biological Bombs over Britain
Definition
germany in WWI
Term

 

, grows cultures of Bacillus anthracis (anthrax) and Pseudomonas mallei (glanders), supplied by the German government, in his Washington D.C. home. 
The agents and an inoculation device are given to sympathetic dockworkers in Baltimore to infect 3000 head of horses, mules, and cattle destined for the Allied troops in Europe
Definition
German-American, Dr. Anton Dilger 1915
Term

Silent Enemy” took greater toll of lives than bullets

   Initiated preventive measures (creosote pills)
   Initiated elaborate sanitation methods (water testing)
   Initiated medical advances (field laboratories)
 
   Initially lost 3-4 men to disease vs 1 to bullets
   After new measures lost 1.2 disease vs 1.5 bullets

 

Definition
Japan Studies the Impact of Disease During War Time
Term

 

“If we can stop the effects of disease from  killing our own troops, why can’t we harness disease to kill our enemies.”

Definition
 Ishii Shiro
Term

 

When the prisoners managed to escape and tell the story to some local residents, the Japanese army decided to build a new facility. This much bigger and more secure death laboratory was constructed here, some 20 km South of Harbin.
The key purpose of this was the development of biological weapons.
Definition
ping fan
Term

 

, an assault team of seventeen officers from Unit 731 sailed for Saipan Island, in the Pacific Ocean, in an attempt to deny U.S. forces access to the airstrip by planting porcelain bombs containing millions of plague-infected fleas. The ship was torpedoed by a U.S. submarine before it reached its target.

Definition
japan 1944
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