Term
What is the cells energy resource? |
|
Definition
|
|
Term
What kind of work do cells do? |
|
Definition
Movement
Biosynthesis
Active Transport |
|
|
Term
What is the first law of thermodynamics? |
|
Definition
Energy can not be created or destroyed |
|
|
Term
What is the second law of thermodynamics? |
|
Definition
Events in the universe that will naturally occur, energy will always be released and will increase disorder creating entropy |
|
|
Term
What is the mechanism that cells use to manage their energy resources? |
|
Definition
The laws of thermodynamics |
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|
Term
Energy can't be created or destroyed but it can be what? |
|
Definition
Converted to different forms of energy |
|
|
Term
|
Definition
The flow of energy in a living system |
|
|
Term
What is energy transduction? |
|
Definition
The conversion of energy from one form to another |
|
|
Term
|
Definition
The study of energy changes |
|
|
Term
How doe the second law of thermodynamics apply to cells? |
|
Definition
conversion of energy will only occur if entropy is increased |
|
|
Term
What does the second law say about ATP? |
|
Definition
When ATP is hydrolyzed some energy is lost as heat not usuable by the cell and some is used to produce movement |
|
|
Term
What is the energy called that the cell uses to do work? |
|
Definition
|
|
Term
What do the variables stand for
H=G+S |
|
Definition
H Amount of energy
S entropy
G is free energy |
|
|
Term
If the final state has more energy than the initial state then the reaction is what?
ΔG>0 |
|
Definition
|
|
Term
If the final state has less energy than the initial state then the process is what?
ΔG<0 |
|
Definition
|
|
Term
|
Definition
The Study of reactions outside the cell |
|
|
Term
|
Definition
The study of reactions inside the cell |
|
|
Term
What is energy coupling in the cell? |
|
Definition
The use of exergonic reactions to drive endergonic reactions |
|
|
Term
What currency do cells use to drive energy coupling? |
|
Definition
|
|
Term
What are the products of ATP? |
|
Definition
|
|
Term
What type of reaction is the hydrolysis of ATP? |
|
Definition
|
|
Term
What is the hydrolysis of ATP going to be used for? |
|
Definition
|
|
Term
What type of molecule is ATP? |
|
Definition
|
|
Term
How much energy does an ATP molecule produce? |
|
Definition
|
|
Term
What reactions can ATP drive and Why? |
|
Definition
Reaction that require less than 7.3 kcal per mol
because some energy will be lost as heat. |
|
|
Term
What is the mechanism of energy transfer of ATP? |
|
Definition
The energy is transfered with the phosphate functional group that is removed through hydrolysis |
|
|
Term
|
Definition
Anything that has had a phosphate functional group added to it |
|
|
Term
What is dephosphorylation? |
|
Definition
Anything that has had a phosphate functional group removed |
|
|
Term
What are enzymes and what are their functions? |
|
Definition
A special class of proteins that cataylze reactions |
|
|
Term
What are the characteristics of enzymes? |
|
Definition
1. they are catalytic proteins
2. they do not effect the free energy transfer
3. They speed up reactions of things that can and will occur.
4. They do not change the amount of energy to start an endergonic reaction
5. They exihibit substrate specificity
6. effective in small amounts
7. one enzyme can catalyze a reaction multiple times
8. They are sensitive to environmental conditions
9. have optimal conditions temp and pH.
10. enzymes can be regulated
11. some require assistance from other molecules such as ions |
|
|
Term
|
Definition
|
|
Term
What is cellular respiration? |
|
Definition
The process by which cells harvest the energy from organic molecules and converting it to ATP |
|
|
Term
|
Definition
An organic non protein component for catalysis |
|
|
Term
|
Definition
Enzymes that remove hydrogen atoms. |
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|
Term
What is the function of NAD+? |
|
Definition
To carry the hydrogen atom broken of by dehydrogenases |
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|
Term
What type of molecule is NAD+? |
|
Definition
|
|
Term
What is the energy of activation? |
|
Definition
The energy required to destabilize bonds in a reaction |
|
|
Term
What do enzymes do to the activation energy? |
|
Definition
|
|
Term
What is the active site of an enzyme? |
|
Definition
The site of the enzyme where the physical interaction of molecules occurs |
|
|
Term
|
Definition
Molecules that will interact with the active site of and enzyme |
|
|
Term
|
Definition
Subtrate bind to the active site then the enzyme goes through a confirmational change that destorts the bonds of the substrates then the products of this all the enzyme to go back to the original confirmation which releases the product. |
|
|
Term
What are in-vivo characteristics? |
|
Definition
Things known about structures or processes inside a living cell. |
|
|
Term
What are in-vitro characteristics? |
|
Definition
Things known about structures or processes outside the living cell |
|
|
Term
|
Definition
The mechanism for characterizing catayltic activity of enzymes |
|
|
Term
What factors are looked at in enzyme kinetics? |
|
Definition
1. substrate specificity
2. at what concentrations of molecules do the enzymes work
3. reaction rate |
|
|
Term
What do we use enzyme kinetics to study? |
|
Definition
|
|
Term
What is initial reaction velocity? |
|
Definition
The change in product over time |
|
|
Term
What is saturation of an enzyme? |
|
Definition
The substrate concentration at which every active site is occupied |
|
|
Term
What is the maximum velocity of an enzyme? |
|
Definition
The reaction velocity at which you reach saturation |
|
|
Term
|
Definition
The measure of how well enzyme is binding substrate |
|
|
Term
What value of km makes it a better fit for the cell? |
|
Definition
|
|
Term
What types of enzyme inhibition are there? |
|
Definition
Irreversible and reversible |
|
|
Term
What are the two mechanisms of reversible inhibition? |
|
Definition
Competitive inhibition and noncompetitive inhibition |
|
|
Term
What is competitive inhibition? |
|
Definition
a molecule that looks like the active site of the enzyme and competes with other substrate to bind with the active site to inhibit the production of that enzyme |
|
|
Term
What happens when you add more substrate than competitive inhibitor? |
|
Definition
|
|
Term
What is noncompetitive inhibition? |
|
Definition
The binding of a molecule to the enzyme at a site that is not the active site which cause the active site to change |
|
|
Term
What is allosteric regulation? |
|
Definition
A mechanism for enzyme regulation that is reversible |
|
|
Term
What is the mechanism for allosteric regulation? |
|
Definition
An inhibitor molecule that binds to a site that is not the active site called the allosteric site and it changes the confirmation of the enzyme to either inhibit or activate enzymes |
|
|
Term
What is the first teir of biosynthesis? |
|
Definition
Breaking down molecules to building block to use them to make molecules of the same kind. |
|
|
Term
What is the second tier of biosynthesis? |
|
Definition
They break down of molecules to Acetyl CoA |
|
|
Term
|
Definition
breaking down a molecule to produce the biosynthetic intermediate to create the same molecule |
|
|
Term
What is the third tier of biosythesis? |
|
Definition
The breakdown of molecules to create energy |
|
|
Term
What does enzyme regulation allow cells to do? |
|
Definition
To control what molecules are being used for |
|
|
Term
What is a metabolic pathway? |
|
Definition
A series of linked reactions |
|
|
Term
What are transient intermediates in metabolic pathways? |
|
Definition
Intermediates that are produced and immediately consumed |
|
|
Term
What are anabolic pathways? |
|
Definition
Pathways in which we assemble small molecules into macromolecules AKA biosynthesis |
|
|
Term
What type of reactions are anabolic pathways? |
|
Definition
|
|
Term
What are catabolic pathways? |
|
Definition
The breakdown of molecules to their simplest components |
|
|
Term
What type of reactions are in catobolism? |
|
Definition
|
|
Term
The control of metabolic pathways are controlled by what? |
|
Definition
Enzyme regulation
usually only one ezyme
and
compartmentation |
|
|
Term
What makes glucose a good energy source? |
|
Definition
The carbon hydrogen bonds
Taking the hydrogen sharing electrons with carbon and replace them with oxygen
we go from high energy electrons to low energy electrons |
|
|
Term
What make electrons high energy? |
|
Definition
When they are being shared equally |
|
|
Term
When is energy realesed in carbon? |
|
Definition
When it is moved to a more oxidized state |
|
|
Term
What are the stages of cellular respiration? |
|
Definition
1. glycolysis
2.TCA cycle, Krebbs cycle, Citric Acid cycle
3. electron transport oxidative phosphorylation |
|
|
Term
Whats the function of glycolysis? |
|
Definition
Breaking down glucose into pyruvate |
|
|
Term
What is the function of pyruvate processing?
(TCA cycle, Krebbs cycle, Citric Acid cycle) |
|
Definition
harvesting high energy electrons with NAD+ |
|
|
Term
What is the functions of electron transport oxidative phosphorylation? |
|
Definition
|
|
Term
What are the three mechanisms for ATP synthesis? |
|
Definition
1.Oxidative phosphorylation
2. photophosphorylation
3. subtrate level phosphorylation |
|
|
Term
What happen in substrate phosphorylation? |
|
Definition
We start with ADP and an organic molecule that has a phosphate functional group. the phophate functional group will be broken off of the substrate and added to ADP to create ATP |
|
|
Term
What things go into and what comes out in glycolysis? |
|
Definition
1 6-carbon Glucose to 2-3 carbon pyruvate
ADP to ATP
NAD+ to NADH |
|
|
Term
What has happened when NADH is created? |
|
Definition
We have moved high energy electrons off of a carbon skeleton. |
|
|
Term
What goe into the TCA cycle and what comes out? |
|
Definition
Pyruvate goes in and CO2 comes out
NAD+ to NADH
FADH2
|
|
|
Term
What goes into electron transport and what comes out? |
|
Definition
O2 goes in H2O comes out
ADP goes in ATP comes out
Electrons carried by NADH and FADH2 and NAD+ comes out and FADH
|
|
|
Term
Where does glycolysis occur? |
|
Definition
|
|
Term
Where do the pyruvate and high energy electrons produced by glycolysis get shuttled? |
|
Definition
|
|
Term
What is the energy harvested by the electron transport chain used to create? |
|
Definition
|
|
Term
What is the potential energy of the proton gradient used for? |
|
Definition
|
|
Term
|
Definition
Enzymes catalyze the reactions that remove Hydrogen atoms. |
|
|
Term
|
Definition
An organic molecule required for dehydrogenase activity |
|
|
Term
What is the dehydrogase going to move and where is it going to attach it? |
|
Definition
A hydride a hydrogen with its electron and carbons
to NAD+ |
|
|
Term
What do kinase enzymes do? |
|
Definition
Transfer phophate functional groups too or from ATP |
|
|
Term
Why do cells phophorylate glucose? |
|
Definition
|
|
Term
What enzyme is in glycolysis in regulated by allosteric regulation? |
|
Definition
|
|
Term
What is the mechanism of making ATP called? |
|
Definition
substrate level phosphorylation |
|
|
Term
What is the Net equation of glycolysis? |
|
Definition
Glucose+2 ADP+ 2 Pi + 2 NAD+→ 2 Pyruvate + 2 ATP + 2 NADH + 2 H+ + 2 H2O |
|
|
Term
|
Definition
Energy harvest exclusively from glycolysis |
|
|
Term
What are the extra step added to glycolysis(in Humans) for fermentation and what are they intended to do? |
|
Definition
pyruvate+ lactate dehydrogenase+NADH+H+→ Lactate + NAD+
Regenerate NAD+ |
|
|
Term
What are the extra steps are added to glycolysis(in yeast) for fermentation? |
|
Definition
1.Pyruvate+ Pyruvate decarboxylase→CO2 + Acetaldehyde
2. Acetaldehyde + NADH + H+ + Alchohol dehydrogenase→ Ethyl alchohol |
|
|
Term
What happen to pyruvate in the presence of oxygen? |
|
Definition
It will cross into the mitochondria and be converted into Acetyl CoA |
|
|
Term
What mechanism of enzyme regulation is glycolysis controlled by? |
|
Definition
|
|
Term
What is the process of feedback inhibition? |
|
Definition
Regulation in response to metabolite concentration |
|
|
Term
What is covalent modification? |
|
Definition
changing the confirmation of the enzyme through a covalent bond. Ex. phosphorylation and dephosphorylation |
|
|
Term
What is the key regulation enzyme in glycolysis? |
|
Definition
|
|
Term
|
Definition
The process of converting pyruvate into glucose |
|
|
Term
In the presence of oxygen what products of glycolysis are shipped to the mitochondria? |
|
Definition
Pyruvate and the high energy electrons carried by NADH |
|
|
Term
What is the endosymbiont theory? |
|
Definition
The theory that the mitochondria and the chloroplast evolved from an early prokaryote inside a cell. |
|
|
Term
What evidence supports the endosymbiont theory? |
|
Definition
The two membranes of the mitochondria where one was the plasma membrane of the cell that consumed it
Mitochondrial DNA that is functional
has its own ribosomes
mitochondria devide independently of the host cell and devide by a process similar to binary fission
|
|
|
Term
What is the pathway of Pyruvate into the mitochondria? |
|
Definition
It crosses the outer membrane then the inner membrane into the mitochondrial matrix. |
|
|
Term
|
Definition
A. Inner membrane
B. Outer membrane
C. Matrix
D. Cristae |
|
|
Term
What is pyruvate processing? |
|
Definition
The conversion of pyruvate to Acetyl CoA |
|
|
Term
How is Pyruvate Converted to Acetyl CoA? |
|
Definition
With the enzyme pyruvate dehydrogenase |
|
|
Term
What is special about FADH? |
|
Definition
it is the only high energy electron acceptor in the TCA cycle and is directly embedded in the electron transport chain |
|
|
Term
How many energy harvesting reactions occur in the mitochondria? |
|
Definition
5
1 in pyruvate processing
4 in the TCA cycle |
|
|
Term
What happens to the electron carried by NADH that were produced in glycolysis? |
|
Definition
NADH passes its electrons to a carrier with the presence of oxygen and they lose a little energy |
|
|
Term
How do electron move through the electron transport chain? |
|
Definition
The electrons pass through the components of the electron transport chain. as they pass through they lose energy in discrete packages. by the time the reach the last component they are now low energy electrons. oxygen then picks up the low energy electrons. the energy that was carried by the high energy electrons is used to create a proton gradient and a voltage as well as a pH greadient. |
|
|
Term
How does the mitochondria produce ATP? |
|
Definition
the mitochondria pumps protons into the inner membreane space due to the Proton gradient created by the electrons of the electron transport chain. In the inner membrane ATP synthase is embedded. ATP has two components. The transmembrane domain acts as a proton channel and when it gets the signal it opens up and allow the protons to diffuse back across the membrane. at the catalytic site the protons Physically attatch Which causes the catalytic site to turn which attatches the phophate to the ADP to make ATP |
|
|
Term
Where does all the ATP made by ATP synthase end up and where does it get transported? |
|
Definition
In the Mitochondrial matrix then it gets transported out to the cytosol |
|
|
Term
What is the proton-motive force? |
|
Definition
The gradient created by protons
which is a pH gradient and an electrochemical gradient |
|
|
Term
|
Definition
The coupling of potential energy of a proton motive force to ATP synthesis |
|
|
Term
What are the components of the electron transport chain? |
|
Definition
|
|
Term
What is complex 1 of the electron transport chain? |
|
Definition
The complex that recieves the high energy electrons from NADH |
|
|
Term
What is complex 2 of the electron transport chain? |
|
Definition
The complex that includes succinate dehydrogenase and accepts the electrons from FADH2 |
|
|
Term
What is complex 3 of the electron transport chain? |
|
Definition
The complex that recieves all the electrons from complex 1 and 2 |
|
|
Term
What is complex 4 of the electron transport chain? |
|
Definition
The complex that recieves all the electrons leaving complex 3 also called cytochrome oxidase
is the final stop
the electrons are low energy that are going to be picked up by oxygen to form water |
|
|
Term
What is significant about the complexed of the electron transport chain? |
|
Definition
They are multiprotein complexes |
|
|
Term
How is the precision of movement in the complexes of the electron transport chain maintained? |
|
Definition
Because electrons are passed to molecules that have a slightly higher electronegativity then eventually they are passed to oxygen which is the most electronegative. |
|
|
Term
|
Definition
The tendency of molecules to give up electrons |
|
|
Term
What redox potential do stron reducing agents have? |
|
Definition
A negative redox potential. |
|
|
Term
What redox potential do weak reducing agents have? |
|
Definition
A positive redox potential |
|
|
Term
What is the reducing agent? |
|
Definition
The molecule that has given up an electron and has become oxidized |
|
|
Term
What is the oxidizing agent? |
|
Definition
The molecule that has accepted an electron and has become reduced |
|
|
Term
What are prostetic groups?
What are conjugated proteins? |
|
Definition
Non protein components.
The protein component |
|
|
Term
What are the functions of protetic groups in the electron transport chain? |
|
Definition
|
|
Term
What is the function of Ubiquinone in the electron transport chain? |
|
Definition
To shuttle electrons from complex 1 to complex 3 and complex 2 to complex 3 |
|
|
Term
Where are packets of energy lost in the electron transport chain and what is this energy used for? |
|
Definition
Complex 1, complex 3, and complex 4.
to be used by other proteins of the protein complexs to pump protons from the mitochondrial membrane to the inner membrane space |
|
|
Term
|
Definition
Organisms able to produce their own food supply and produce the worlds food supply |
|
|
Term
|
Definition
Organisms dependent on the food supply created by the autotrophs |
|
|
Term
|
Definition
The inner most part of the chloroplast |
|
|
Term
|
Definition
|
|
Term
Within the stroma is a third set of membrane called what? |
|
Definition
|
|
Term
What is the interior of thylakoid called? |
|
Definition
|
|
Term
Where is light energy and ATP harvested and made in the chloroplast? |
|
Definition
Within the membrane of the thylakoid |
|
|
Term
What is a stack of thylakoid called? |
|
Definition
|
|
Term
What are high energy electrons carried by in plant cells? |
|
Definition
|
|
Term
What is the purpose of light dependent reactions? |
|
Definition
To harvest light energy to convert it to ATP and to harvest reducing equivalents carried by NADPH |
|
|
Term
What is the purpose of Light-Independent Reactions? |
|
Definition
Carbon fixation and synthesis of CHO |
|
|
Term
What type of reaction is Photosynthesis? |
|
Definition
|
|
Term
What type of reaction is cellular respiration? |
|
Definition
|
|
Term
What happens in light dependent reactions? |
|
Definition
Plant use energy form sunlight to make ATP and to convert low energy electron to high energy electrons NADPH |
|
|
Term
What is the Calvin cycle? |
|
Definition
The metabolic pathway of converting CO2 to carbohydrate |
|
|
Term
|
Definition
The conversion of carbon dioxide to an organic molecule |
|
|
Term
Where do High energy electron come from in the light dependent reactions? |
|
Definition
|
|
Term
What is the energy of light dependent on? |
|
Definition
The wavelength of light
a higher wavelength has lower energy |
|
|
Term
What is the function of photosynthetic pigments? |
|
Definition
Pigments that have the ability to harvest energy form light but only in a certain range of light wavelength |
|
|
Term
|
Definition
A package of photosynthetic pigments in a chloroplast |
|
|
Term
Where is light energy collected by the photosystem sent to? |
|
Definition
|
|
Term
How many photosystem are in Photosynthesis? |
|
Definition
|
|
Term
Where does photosynthesis begin? |
|
Definition
|
|
Term
What happens in photosystem 2? |
|
Definition
1.Light energy harvested is sent to the reaction center.
2. at the reaction center low energy electrons from water(photolysis)are converted to high energy electrons. which is an endergonic process
3.The electrons are physically moved in the reaction center to convert them into high energy electrons using redox reactions.
4. The high energy electrons leave photosystem 2 and are passed off to an electron transport chain. Which functions just like a mitochondrial electron transport chain |
|
|
Term
What happens in photosytem 1? |
|
Definition
1. as the electrons move throught the electron transport chain they lose energy and create a proton motive force which is used to make ATP via Photophosphorylation which is catalyzed by ATP synthase.
2. the electrons are passed to photosystem 1
3. Photosystem 1 uses light to convert low energy electrons to high energy electrons and passes them off to NADPH |
|
|
Term
|
Definition
The splitting of water to produce oxygen, electrons, and protons in photosynthesis |
|
|
Term
What drives the movement of electron from water in photosynthesis? |
|
Definition
The higher electronegativity of the reaction center of photosystem 2 |
|
|
Term
|
Definition
An electron shuttler in the chloroplast electron transport chain. |
|
|
Term
Where will ATP made in the chloroplast go? |
|
Definition
It will stay and be consumed in the stroma |
|
|
Term
What is noncyclic photophosphorylation? |
|
Definition
ATP produced by the flow of electrons from ps2 to ps1 |
|
|
Term
What is cyclic photophosphorylation? |
|
Definition
Electrons that are recycled back to ps1 through the electron transport chain to produce a little more ATP without making more NADPH |
|
|
Term
What is the oxygen we breathe a product of? |
|
Definition
The light dependent reactions in photosynthesis |
|
|
Term
What is Ribulosebisphosphate carboxylase and what is its function? |
|
Definition
Otherwise known as rubisco in the calvin cyclye it attatches carbon dioxide to ribulosebisphophate. |
|
|
Term
What happens in the first step of the calvin cylcle? |
|
Definition
RUBISCO attaches CO2 to ribulose bisphospate which immediatly breads down into 2 3-phosphoglycerate |
|
|
Term
What happens in stage two of the calvin cycle? |
|
Definition
3-phophoglycerate + ATP+NADPH to 1,3-bisphosphoglycerate
1,3 biphosphoglycerate to glyceraldehyde 3-phosphate |
|
|
Term
What is the third stage of the calvin cycle? |
|
Definition
The use of ATP to regenerate RUBP |
|
|
Term
What is C3 photosynthesis? |
|
Definition
The process of taking CO2 from the atmosphere and converting it into two molecules of phosphoglycerate. |
|
|
Term
What is CAM photosynthesis?
|
|
Definition
the process of fixing carbon dioxide to malate at night then break it down in the morning |
|
|
Term
What is C4 photosynthesis? |
|
Definition
the process of seperating CO2 with rubisco and conversion of it to malate |
|
|