Shared Flashcard Set

Details

Test 2 Review
Chapters 3.1, 4, 7, 24,25
92
Microbiology
Undergraduate 2
02/06/2012

Additional Microbiology Flashcards

 


 

Cards

Term
culturing microorganisms
Definition
inovulsyion, invubsyion, idolsyion, inspection, and identification
Term
Inoculation
Definition
the introduction of a microorganism to a medium
Term
Isolation
Definition
the separation of one particular cell to create a colony
Term
Media
Definition
classified by: physical state, chemical composition, and purpose, functional type.
Term
physical states of media
Definition
liquid (nutrient broth); semisolid (agar or gelatin)
Term
fastidious
Definition
bacteria that require growth factors and complex nutrients (pH)
Term
Staphylococcus (exceptions?)
Definition
grows well in mannitol salt agar, high concentration of NaCl(7.5%); quite inhibitory to most human pathogens
Term
Bile salts (MacConkey agar)
Definition
inhibit gram-positive but permit gram-negative; used to isolate intestinal pathogens
Term
Reducing media
Definition
absorbs oxygen thus slows penetration of oxygen in medium; important for growing anaerobic bacteria &/or determining the O req's of isolates
Term
Incubation
Definition
when a inoculated medium is encouraged to multiply in a specific setting
Term
Inspection
Definition
determining if the sample is pure (axenic), mixed, or contaminated
Term
Identification
Definition
determining exactly what colony(ies) were grown
Term
Cellular Organization of Prokaryotic Cell
Definition
External (Appendages: flagella, pili, fimbriae --Glycocalyx: Capsule (slime layer); Cell envelope (, cell wall, cell membrane); Internal (Cytoplasm, ribosomes, inclusions, nucleoid/chromosome, Actin cytoskeleton, endospore)
Term
Prokaryotes
Definition
do not have nucleus, no histones, unique cell wall made of peptidoglycan, no organelles, only some have internal membrane
Term
Eukaryotes
Definition
have nucleus, DNA wind around histones, have organelles in cytoplasm
Term
Archae
Definition
tough walls made up of other chemicals
Term
Glycocalyx
Definition
external to cell wall (made up of layer of molecules); serves as protective, adhesive, and receptor
Term
Baterial chromosome or nucleoid
Definition
made up of condensed DNA; directs genetics, heredity, and codes proteins
Term
Pilus
Definition
appendage; transfers DNA to other cells
Term
Plasmid
Definition
doulble-stranded DNA circle containing extra genes
Term
Ribosomes
Definition
composed of protein and RNA; protein synthesizers
Term
Actin cytoskeleton
Definition
proteins that encircle the cell just inside the membrane; for structure/shape
Term
Flagellum
Definition
specialized appendage attached by basal body; Movement
Term
Fimbriae
Definition
hair-like; for adhesion to other cells & absorption of nutrients
Term
Inclusion/Granule
Definition
store nutrients for later use
Term
Cell wall
Definition
casing that provides structural support and shape
Term
Cell (cytoplasmic) membrane
Definition
lipid and protein sheet that surrounds cytoplasm; controls flow of materials in and out of cell pool
Term
Outer membrane
Definition
extra membrane containing lipopolysaccharides; controls flow of materials, can be toxic to mammals
Term
Endospore
Definition
dormant body; formed to allow survival in adverse conditions
Term
Cytoplasm
Definition
water-based solution fills cell
Term
Bacteria
Definition
all have cell membrane, cytoplasm, ribosomes, and chromosome(s)
Term
Appendages
Definition
absorption
Term
Polar flagella arrangement
Definition
attached at one or both ends; three subtypes: mono-(one), lopho-(bunches at same site), and amphi- (both poles) trichous
Term
Flagellum
Definition
for motility; three parts: hook, filament, and basal body (point of attachment); vary in polar and peritrichous arrangements
Term
Chemotaxis
Definition
positive or negative movement of a cell in a favorable direction
Term
Run
Definition
smooth linear direction towards stimulus
Term
Tumble
Definition
reverse direction of flagella and causes cell to stop and change course
Term
Phototaxis
Definition
movement in response to light
Term
Gram-positive
Definition
structure like open faced sandwich; two layers, thick cell wall (peptidoglycans) and cytoplasmic membrane
Term
Gram-negative
Definition
complete sandwich structure; three layers: outer, cell wall, and cytoplasmic membrane
Term
Mycoplasma
Definition
bacteria lacking cell wall
Term
Mycoplasma pneumoniae
Definition
most important medical species; adheres to epithelial cells in lung, cuases atypical form of pneumonia
Term
Bacterial choromosome
Definition
single circlular strand in which hereditary material exists
Term
Nucleoid
Definition
houses DNA in bacteria; aggregated dense area of cell
Term
Plasmids
Definition
non-essential pieces of DNA
Term
Cell Shapes
Definition
coccus (spherical), bacillus (cylindrical), vibrio (gently curved), spirillum (slightly curled or spiral shaped cylinder); pleomorphism(species often vary in shape and size)
Term
Taxonomic Scheme (major divisions)
Definition
Gracilicutes (gram-neg); Fimicutes (gram-pos); Tenericutes (lack cell wall/soft); and Medosicutes (archae, w/unusual everything)
Term
Aerobic
Definition
use oxygen to metabolize
Term
Anaerobic
Definition
do not use oxygen to metabolize
Term
Facultative bacteria
Definition
may or may not use oxygen to metabolize
Term
Essential nutrient
Definition
any substance that must be provided to an organism
Term
Inorganic nutrient
Definition
simple molecule/atom that does not contain H or C
Term
Oranic nutrients
Definition
contain C and H and are usually products of living things
Term
Escherichia coli
Definition
intestinal bacterium
Term
Principle Elements
Definition
C, O, N, H, P, and S
Term
Heterotroph
Definition
organism that must obtain its carbon in an organic form
Term
autotroph
Definition
"self-feeder"; uses inorganic CO2 as its carbon source; not dependent on living things
Term
Nitrogen
Definition
79% of earth's atmosphere; need to be converted into basic building blocks or NH3(the only form that can be directly combined with C to synthesize amino acids)
Term
Hydrogen performs roles of
Definition
maintaining pH, forming hydrogen bonds, source of free energy in oxy-reduc reations of respiration
Term
Phosphorus
Definition
found in ATP; serves in cellular energy transfers
Term
Photoautotroph
Definition
energy source: sunlight; eg. algae
Term
Chemoautotroph
Definition
energy source: simple inorganic chemicals; eg. deep-sea vent bacteria
Term
Chemoheterotroph
Definition
energy source: metaabolic conversion from other organisms; eg. protozoa, fungi
Term
Saprobe
Definition
energy source: metabolizing the organi matter of dead organisms; eg. fungi, bacteria
Term
Parasite
Definition
energy source: utilizing live host; eg. bacteria, fungi, protozoa, animals
Term
Photoheterotroph
Definition
energy source: sunlight; eg. purple and green bacteria
Term
Growth factor
Definition
an organic compound that must be provided a certain nutrient to synthesize
Term
isotonic
Definition
environment is equal in solute concentration
Term
Hypotonic
Definition
solute concentration of external environment is lower than that of cell's internal (bloats)
Term
Hypertonic
Definition
the environment has a higher solute concentration than the cytoplasm
Term
Facilitated diffusion
Definition
bonding of a molecule that causes changes which facilitate the molecule's passage across the membrane and make the cell release the molecule
Term
Active transport
Definition
transport or nutrients again the diffusion gradient; presence of specific membrane proteins and pumps; and the expenditure of energy
Term
Optimum temperature
Definition
promotes the fastest rate of growth and metabolism
Term
Psychrophile
Definition
microorganism that has an optimum temp. below 15C and is capable of growth at 0C
Term
Mesophiles
Definition
organisms that grow at intermediate temperatures (20C-40C)
Term
Thermophiles
Definition
grow optimally at temps greater than 45C
Term
Red snow
Definition
Chlamydomonas nivalis; classified as a "green" alga, but most times is red pigmented
Term
pH of organisms
Definition
majority between 6 and 8
Term
Osmophiles
Definition
live in habitats with high solute concentration
Term
Halophiles
Definition
perfer high concentrations of salt
Term
Barophiles
Definition
exist under extreme pressures
Term
Symbiosis
Definition
two organism live together in a partnership
Term
Mutualism
Definition
exists in organisms that benefit from obligatorily with each other
Term
Commensalism
Definition
receieves benefits while is coinhabitant is neutral
Term
Satellitism
Definition
one member provides nutritional or protective factors needed by the other
Term
Synergism
Definition
interrelationship between two or more free-living organism that benefit them but is not necessary for their survival
Term
Biofilms
Definition
80% of chronic infections
Term
Lag phase
Definition
when a population appears not to be growing or is gwoing at less than the exponential rate (stage 1 normal growth curve)
Term
Exponential growth phase
Definition
phase continues upwards as long as cells meet their needs; maximum rate of cell division (stage 2 normal growth curve)
Term
Staionary growth phase
Definition
population enters survival mode; cells stop growing or slowly (stage 3 normal growth curve)
Term
Death phase
Definition
when limiting factors intensify and death of cells occurs
Term
Turbid
Definition
when a nutrient solution becomes cloudy because of growing microbes
Supporting users have an ad free experience!