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Test 1--MICRO
Dr White--UCF
41
Microbiology
Undergraduate 3
02/11/2012

Additional Microbiology Flashcards

 


 

Cards

Term
Linean System (1735)
Definition

2 Kingdoms: 

 

Animalia

Plantae

Term
Linean Kingdom: Animalia
Definition
motile, nonphotosynthetic
Term
Linean System: Plantae
Definition
nonmotile, photosynthetic
Term
Haeckel (1866)
Definition

3 Kingdoms: 

 

Animalia

Plantae

Protista

Term
Haeckel: Protista
Definition

simple organization, not easily placed in other 2 kingdoms. 

 

Unicellular

Multicelluar do not develop differential tissue

 

algae, fungi, protozoa, and bacteria + blue-green algae

Term
Stainer Division
Definition

Higher Protista

Lower Protista

Term
Stainer: Higher Protista
Definition

Eukaryotic cell structure- membrane bound nucleus

 

Algae, protozoa, fungi

Term
Stainer: Lower Protista
Definition

Prokaryotic cell structure--> no membrane bound nucleus

 

cyanobacteria (blue-green algae) and bacteria

Term
Whittaker's System (1969)
Definition

5 Kingdoms

I. Monera- prokaryotic & unicellular

--bacteria & cyanobacteria

II. Protista- eukaryotic &unicellular 

--algae & protozoa

III. Plantae - photosynthesis

IV. Fungi - absorption

V. Animalia- Ingestion

Term
Woese's 3 Domain's
Definition

Discovery of Archaea through ribosomal RNA

 

I. Bacteria (Eubacteria) --> All prokaryotes

Gram +, Gram - , Cyanobacteria

II. Archaea--> All prokaryotes

Methanogens, Hyperthermophiles, Halophiles

III. Eukarya--> All eukaryotes

Term

Staining Properties: 

 

Morphology

Definition
Obtained by simple stain or negative stain
Term

Staining Properties: 

 

Differential Stain

Definition

Gram reaction--> Gram +/-

 

Specific Enzymes

Term

Staining Properties: 

 

Structual Stain

Definition

Spore: Clostridium

 

Capsule 

 

Flagellum

Term
Alcohol
Definition
-OH hydroxyl group
Term
Aldehyde
Definition

C=O, also bonded to H and R group

 

C=O is called carbonyl group

Term
ketone
Definition

C=O, also bonded to 2 other R groups

 

C=O is called a carbonyl group

Term
Carboxylic Acid
Definition

C double bonded to a O, also bonded to an -OH, also bonded to an R group

 

-COOH is called carboxyl group, in water loses H+ ion

Term
Esters
Definition

Formed by combining an acid and an alcohol

 

Acid + Alcohol = Ester + Water

Term

C-N Compunds: 

 

Amines

Definition
[image]
Term

C-N Compunds: 

 

Amides 

Definition

combining an acid and an amine, uncharged in water

 

-COOH + NH2 --> -NH-C + H2O

Term
Phosphates
Definition

Know high energy bonding

 

Inorganic phosphate written as Pi

Term

Hydrogen Bonding

 

C/G > A/T

or

C/G

Definition

Cytosine/Guanine has 3 sites of hydrogen bonding 

 

where Adenine/Thymine only have 2

 

Therefore C/G is more stable DNA stranding

Term
What percentage H20 and what percentage chemicals in cell?
Definition

About 70% H2O 

About 30% chemicals--most of which are proteins

Term
Carbohydrates (sugars)
Definition

Carbon, Hydrogen, Oxygen 

 

in ratio of 1:2:1

 

Monosaccharides and Polysaccharides

 

GLYCOSIDIC BONDING

Term
Starch and Glycogen Contain _____ Bonding
Definition
Alpha Glycosidic Bonding
Term
Cellulose contains _____ bonding
Definition
Beta glycosidic bonding
Term
Lipids
Definition

Non-polar macromolecules

 

Ex. triglycerides, waxes, sterols, fatty acids

Term
Nucleic Acids
Definition

3 Units: 

 

5 Carbon Sugar (ribose, RNA, or deoxyribose, DNA)

A nitrogen base

molecule of Phosphate (PO42-)

 

Contain N- glycosidic linkages

 

Carbon 3 prime - Carbon 5 prime linkages

 

Phosphate likage= phosphodiester

Term
Pyrimidine Bases
Definition

Single 6 membered ring 

 

Cytosine 

Uracil

Thymine

Term
Purine Bases
Definition

2 fused heterocyclic (more than 1 kind of atom) rings

 

Adenine 

Guanine

Term
DNA
Definition

carries gentic blueprint for cell

 

double stranded

 

strands associate by hydrogen bonding

Term
RNA 
Definition

Intermediate blueprint-> amino acid sequence

 

single stranded molecules

 

2ndary structure due to H bonding

 

mRNA --> messenger RNA

tRNA --> transfer RNA

rRNA --> ribosomal RNA

Term
RNA made off ___-___ DNA
Definition
Made off 3'-5' DNA strand
Term
Amino Acids
Definition

Contain carboxylic acid (-COOH) and a amino group (-NH2)

 

Monomeric units of proteins

 

tetrhedral structures

Term
Peptide Bonding
Definition
Carboxylic group of one amino bonding to the nitrogen group of a second amino
Term
Most Prevalent Biological Isomers
Definition

L-Amino Acids

 

D-Sugars

Term

Protein Structure: 

 

Primary

Definition
linear sequence of amino acids, peptide bonding (covalent bonding)
Term

Protein Structure: 

 

Secondary

Definition

interactions between polypeptide backbone

 

alpha helix and beta pleated sheets

 

hydrogen bonding

Term

Protein Structure: 

 

Tertiary 

Definition

overall conformation stabilized by weak interactions

 

(final folding in 3D space) 

 

disulfide bridges in 1 polypeptide

Term

Protein Structure: 

 

Quaternary

Definition

proteins of multiple polypeptides

 

linking of 2 polypetides by disulfide bridge

Term
Denaturation
Definition

proteins exposed to extreme heat, pH, chemicals

 

polypeptide chain unfloding

 

still holds primary structure

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