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Test 1- Gram Pos Cocci
Strep and Staph
66
Microbiology
Undergraduate 4
08/29/2009

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Cards

Term
What part of the cell differentiates gram positive from gram negative organisms?
Definition
the cell wall
Term
What kind of bacterial walls contain peptidoglycan?
Definition
All bacteria except Mycoplasma
Term
What kind of bacterial cell walls contain teichoic acids?
Definition
Gram positive bacteria only
Term
What kind of bacterial cell walls contain lipopolysaccharide?
Definition
Gram negative bacteria only
Term
Which has a thicker cell wall- gram positive or gram negative bacteria?
Definition
Gram positive
Term
How many layers are in a gram positive cell wall?
Definition
2
Term
How many layers are in a gram negative cell wall?
Definition
3
Term
Which kind of bacterial cell wall has more peptidoglycan?
Definition
Gram positive has more than 50% while gram neg has 10-20% peptidoglycan
Term
Which bacteria are sensitive to penicillin- gram positive or gram negative?
Definition
Gram positive bacteria are sensitive to penicillin
Term
Which kind of bacteria contain protein- gram negative or gram positive?
Definition
gram negative
Term
What color do gram positive bacteria stain?
Definition
purple
Term
What color do gram negative bacteria stain?
Definition
pink
Term
What are the two types of pili?
Definition
common pili and sex or F pili
Term
what kind of pili are multiple on cell surface and used for attachment?
Definition
common pili
Term
What does it mean if a bacteria is F+?
Definition
Bacteria that carry the F plasmid are F+. The F plasmid encodes enzymes and proteins that form the sex pilus
Term
What word describes a single flagellum at one end?
Definition
Monotrichous
Term
Small bunches of flagella arising from one end of the cell
Definition
Lophotrichous
Term
Having flagella at both ends of the cell
Definition
Amphitrichous
Term
Having flagella dispersed over the surface of the cell
Definition
Peritrichous
Term
What flagellar arrangement results in the slowest motility?
Definition
Peritrichous
Term
How does the capsule protect the bacterial cell?
Definition
by blocking phagocytosis
Term
What is the function of plasmids?
Definition
to transfer genetic information between organisms
Term
bacterial transmission from person to person
Definition
direct- horizontal
Term
bacterial transmission from mother to baby
Definition
direct- vertical
Term
transmission of bacteria by a vehicle, airborne, or vector-borne
Definition
indirect
Term
Four bacterial virulence properties
Definition
capsule formation, enzyme production, adherence factors, toxin production
Term
what kind of toxins are produced by gram negative bacteria?
Definition
endotoxins
Term
when are endotoxins released?
Definition
when the cell lyses
Term
what kind of bacteria releases exotoxins?
Definition
gram positive
Term
when are exotoxins released?
Definition
exotoxins are released by living bacteria to target specific host cells
Term
bacteria that require oxygen to live
Definition
obligate aerobes
Term
bacteria that will only grow when oxygen is present in amounts below 0.2 atm
Definition
microaerophilic
Term
bacteria that will only grow when oxygen is not present
Definition
obligate anaerobes
Term
bacteria that do not require oxygen for growth but utilize it when it's available
Definition
facultative anaerobe
Term
Is oxygen required for fermentation?
Definition
no
Term
what is produced during fermentation?
Definition
high concentration of strong acids
Term
what are the 5 types of media?
Definition
Supportive, selective, differential, enrichment, enriched
Term
what kind of media contains general nutrients, supports the growth of non-fastidious bacteria, and does not give one organism an advantage?
Definition
supportive media
Term
Nutrient broth/agar, trypticase soy agar, and luria burtoni are examples of what kind of media?
Definition
supportive media
Term
what kind of media contains inhibitory agents such as dyes, bile salts, alcohol, acid or antibiotics?
Definition
selective media
Term
mannitol salt agar, hektoen-enteric agar, phenylethyl alcohol agar and campy blood agar are all examples of what kind of media?
Definition
selective media
Term
what kind of media has distinguishing factors to differentiate bacteria?
Definition
Differential media
Term
MacConkey agar, Mannitol-salt agar, blood agar and triple sugar iron agar are all examples of what kind of media?
Definition
differential media
Term
what kind of media contains required nutrients for one particular pathogen?
Definition
enrichment media
Term
buffered charcoal yeast extract agar, enriched thayer martin agar, and selonite broth are all examples of what kind of media?
Definition
enrichment media
Term
what kind of media contains factors to aid growth of fastidious organisms?
Definition
enriched media
Term
blood agar and chocolate agar are both examples of what kind of media?
Definition
enriched media
Term
What is the first test used to differentiate staphylococcus species?
Definition
coagulase test
Term
What test differentiates between streptococcus and staphylococcus?
Definition
catalase test
Term
Is Staph aureus coagulase positive or negative?
Definition
coagulase positive
Term
What are the two coagulase negative staphylococci most likely to cause disease?
Definition
S. epidermidis and S. saprophyticus
Term
What is the most virulent Staph species?
Definition
S. aureus
Term
What is the function of techoic acid?
Definition
It mediates adherence as part of the cell wall
Term
Does Staph aureus ferment mannitol?
Definition
yes.
Term
When Staph aureus is grown on mannitol agar, what color is produced?
Definition
yellow
Term
What kind of hemolysis would you see with Staph aureus?
Definition
Beta hemolysis
Term
What kind of hemolysis would you see with Staph aureus?
Definition
Beta hemolysis
Term
What is hyaluronidase?
Definition
virulence factor of staph aureus that digests connective tissue- spreading factor
Term
What is staphylokinase?
Definition
virulence factor of s. aureus- enzyme that digests blood clots
Term
What gene is responsible for methicillin resistant staph aureus?
Definition
mec gene
Term
Which staph species causes urinary tract infections?
Definition
Staphylococcus saprophyticus
Term
What test uses H2O2 to see if the organism changes it into H2O and O2?
Definition
Catalase test
Term
Which staph species is resistant to novobiocin?
Definition
staph saprophyticus. all others are sensitive
Term
What group is Streptococcus pyogenes?
Definition
Group A
Term
What group is Streptococcus agalactiae?
Definition
Group B
Term
What are the two categories of Group D Strep?
Definition
Enterococcus and nonenterococcal Group D
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