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Reasons to use microorganisms |
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life processes: metabolic functions starting with genetic code Ease of use Quick generation times Cheap |
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Food, drugs, medicine, gardens, biotechnology |
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Association of microbial communities |
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Earth was originally anoxic |
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No oxygen, carbon and nitrogen were predominant |
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What was on earth originally |
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Anaerobic microorganisms, Cyanobacteria |
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Last universal common ancestor |
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Anything with the ability to cause human disease |
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First to observe microorganisms, fruiting structures of mold |
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Built the first microscope |
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Discovered the bacterial endospores, bacillus anthracis, clostridium botulinum and tetanus |
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Disproved spontaneous generation, pasteurization in foods |
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Observed presence of bacteria in blood of infected animals (germ theory), 4 postulates |
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Enrichment culture techniques, adjustment of nutrient conditions |
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DIscovered nitrifying and sulfur bacteria , discovered concept of nitrogen fixation, Winogradsky column |
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Low magnitude, cheap, little training |
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High magnitude, look at cells and subcells organs, special training, very expensive |
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Interior aqueous environment that all cellular and molecular components reside in |
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Rigid structure usually composed of some kind of polysaccharide with proteins embedded here and there |
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Distinguishing characteristic of membrane bound organells |
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Only in eukaryotes, site of DNA replication, and transcription |
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Where do transcription and translation occur in prokaryotes |
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Definition
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Not, considered living, smaller than bacteria, requires host to replicate and change, can DNA or RNA, no metabolic capabilities without host |
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Particular stretch of DNA |
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Definition
Collective arrangement of genes |
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Mostly circular, single genome with single copy, forms nucleoid
Gene --> Chromosome --> Genome |
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Contain nonessential genes, codes for special advantages, can have multiple plasmids |
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Linear DNA, vary in numbers, organized chromosomes, typically diploid |
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Definition
Bacteria, eukarya, archaea |
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Different requirements for acquiring energy, results in different metabolic strategy to conserving energy |
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Organic chemicals, inorganic chemicals, light energy |
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Conserve energy from organic compounds, ATP, oxygen requirements |
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Inorganic chemical energy |
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Oxidation of inorganic compound to store energy in form of ATP, only in prokaryotes |
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Contain pigments capable of capturing the light energy from the sun |
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Definition
Cyanobacteria- algae Anoxygenic- green bacteria |
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Definition
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Heterotrophs energy source |
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Metabolize organic material
Chemolithotrophs phototrophs |
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Definition
Found in every environment |
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Microorganisms exist (survive/thrive) under conditions that would not usually support life |
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Not dye out, ex: go dormant |
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Living in optimal environment |
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All three types of energy sourcing, primative mitochondria |
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Stain purple, thick peptidoglycan cell wall |
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Phylogenetically most closely related to gram pos, oxygenic phototrophs |
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How to describe colonies on plates |
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Single bonded C and H chains, less fluid |
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C and H chain with a double bond, more fluid |
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More fluid, less dense so don't pack as well |
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General composition is a phospholipid bilayer , charged heads with fatty acid tails |
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Permeable membrane, hydrophobic barrier, charged ions cannot diffuse through |
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Definition
Have both hydrophobic and hydrophilic, firmly embedded proteins or attached to the surface |
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Outer surface interacts with substrates, inner surface interacts with proteins and cytoplasmic membrane |
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Work against the conc gradient, functionally import and export molecules across membrane, required energy |
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One molecule going in or out |
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Two coming in or two going out |
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Two molecules, one goes in and one comes out |
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Simple transporter (uni, sym) |
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Just goes in/ just goes out, energy dependent, uses protein motive force |
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Chemical modification of product going in,phosphotransferase system |
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ATP binding class, hydrolyzing ATP |
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Transporters function to bring something in |
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Lots of transport of different compounds and molecules, highly charged, significant gradient |
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Comes from side cell wall with water chains |
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The cell wall in bacteria cells, sugar molecules and amino acids |
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Typically have a single, thick layer of peptidoglycan |
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Complex cell wall, which consists of a outer membrane, and a thin layer of peptidoglycan and an inner membrane |
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Covalently bonded to NAM, neg a charges of the phosphate contributes to overall neg charge of the cell wall |
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The space in gram negative that surrounds the peptidoglycan, cellular envelope, contains important proteins |
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Responsible for the core, and O-polysaccharide, very antigenic |
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Lipid bilayer is composed of LPS layer and a typical inner phospholipid bilayer |
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Span across the whole membrane, more nonspecific than transporters |
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Anthracis/Cereus/Subtilis |
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Sticky secretion on bacterial surface used as protection |
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Attachment, conjuction, motility |
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Subcellular Storage Components |
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Definition
Carbon storage goes to energy |
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Bacteria are able to align with magnetic fields |
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Visualized of blooms, as they feel the sun they float to the surface |
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Formed during process called sporulation under unfavorable condition |
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Found only in spores, binds to H2O to dehydrate spore |
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Small acid soluble proteins, tightly bind DNA, carbon and energy source |
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Occurs only with environmental stress |
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Form of specialization, senses the other "siblings" in area to start producing the effect |
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Flows from high to low density |
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