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Test 1
Chapter 1,2
99
Microbiology
Undergraduate 4
09/29/2016

Additional Microbiology Flashcards

 


 

Cards

Term
Central Dogma
Definition
DNA --> RNA --> Protein
Term
Reasons to use microorganisms
Definition
life processes: metabolic functions starting with genetic code
Ease of use
Quick generation times
Cheap
Term
Bad microorganisms
Definition
Infectious diseases
Term
Good microorganisms
Definition
Food, drugs, medicine, gardens, biotechnology
Term
Ecosystem
Definition
Association of microbial communities
Term
Evolution
Definition
Earth was originally anoxic
Term
Anoxic
Definition
No oxygen, carbon and nitrogen were predominant
Term
What was on earth originally
Definition
Anaerobic microorganisms, Cyanobacteria
Term
L.U.C.A
Definition
Last universal common ancestor
Term
Pathogens
Definition
Anything with the ability to cause human disease
Term
Robert Hook
Definition
First to observe microorganisms, fruiting structures of mold
Term
Antoni Van Leeuwenhoek
Definition
Built the first microscope
Term
Ferdinand Cohn
Definition
Discovered the bacterial endospores, bacillus anthracis, clostridium botulinum and tetanus
Term
Louis Pasteur
Definition
Disproved spontaneous generation, pasteurization in foods
Term
Robert Koch
Definition
Observed presence of bacteria in blood of infected animals (germ theory), 4 postulates
Term
Martinus Biejernick
Definition
Enrichment culture techniques, adjustment of nutrient conditions
Term
Sergei Winogradsky
Definition
DIscovered nitrifying and sulfur bacteria , discovered concept of nitrogen fixation, Winogradsky column
Term
Light microscope
Definition
Low magnitude, cheap, little training
Term
ELectron microscope
Definition
High magnitude, look at cells and subcells organs, special training, very expensive
Term
Lenses
Definition
How close you can get
Term
Resolution
Definition
How clear it is
Term
Cell membrane
Definition
Permeable barrier
Term
Cytoplasm
Definition
Interior aqueous environment that all cellular and molecular components reside in
Term
Cell wall
Definition
Rigid structure usually composed of some kind of polysaccharide with proteins embedded here and there
Term
Distinguishing characteristic of membrane bound organells
Definition
Nucleous
Term
Nucleous
Definition
Only in eukaryotes, site of DNA replication, and transcription
Term
Where do transcription and translation occur in prokaryotes
Definition
Cytoplasm
Term
Viruses
Definition
Not, considered living, smaller than bacteria, requires host to replicate and change, can DNA or RNA, no metabolic capabilities without host
Term
Gene
Definition
Particular stretch of DNA
Term
Genome
Definition
Collective arrangement of genes
Term
Prokaryote genomes
Definition
Mostly circular, single genome with single copy, forms nucleoid

Gene --> Chromosome --> Genome
Term
Plasmids
Definition
Contain nonessential genes, codes for special advantages, can have multiple plasmids
Term
Eukaryotic genomes
Definition
Linear DNA, vary in numbers, organized chromosomes, typically diploid
Term
3 domains of life
Definition
Bacteria, eukarya, archaea
Term
Microbial diversity
Definition
Different requirements for acquiring energy, results in different metabolic strategy to conserving energy
Term
3 sources of energy used
Definition
Organic chemicals, inorganic chemicals, light energy
Term
Organic chemical energy
Definition
Chemoorganotrophs
Term
Chemoorganotrophs
Definition
Conserve energy from organic compounds, ATP, oxygen requirements
Term
Inorganic chemical energy
Definition
Chemolithotrophs
Term
Chemolithotrophs
Definition
Oxidation of inorganic compound to store energy in form of ATP, only in prokaryotes
Term
Light energy
Definition
Phototrophs
Term
Phototrophs
Definition
Contain pigments capable of capturing the light energy from the sun
Term
Two major phototrophs
Definition
Cyanobacteria- algae
Anoxygenic- green bacteria
Term
Carbon sources
Definition
C,H,N,O,P,S
Term
Heterotrophs energy source
Definition
Chemoorganotrophs
Term
Autotrophs energy source
Definition
Metabolize organic material

Chemolithotrophs phototrophs
Term
Ubiquitous
Definition
Found in every environment
Term
Extreme conditions
Definition
Microorganisms exist (survive/thrive) under conditions that would not usually support life
Term
Survive
Definition
Not dye out, ex: go dormant
Term
Thrive
Definition
Living in optimal environment
Term
Proteobacteria
Definition
All three types of energy sourcing, primative mitochondria
Term
Gram-Positive Bacteria
Definition
Stain purple, thick peptidoglycan cell wall
Term
Cyanobacteria
Definition
Phylogenetically most closely related to gram pos, oxygenic phototrophs
Term
Spherical/cocci
Definition
Most gram-pos
Term
Rod/bacilli
Definition
Most gram-neg
Term
Colony morphology
Definition
How to describe colonies on plates
Term
Saturated
Definition
Single bonded C and H chains, less fluid
Term
Unsaturated
Definition
C and H chain with a double bond, more fluid
Term
Side chains
Definition
More fluid, less dense so don't pack as well
Term
Cytoplasmic membrane
Definition
General composition is a phospholipid bilayer , charged heads with fatty acid tails
Term
Membrane function
Definition
Permeable membrane, hydrophobic barrier, charged ions cannot diffuse through
Term
Membrane Protein
Definition
Have both hydrophobic and hydrophilic, firmly embedded proteins or attached to the surface
Term
Phobic
Definition
Protein in
Term
Philic
Definition
Protein on surface
Term
Functional domains
Definition
Outer surface interacts with substrates, inner surface interacts with proteins and cytoplasmic membrane
Term
Transport Proteins
Definition
Work against the conc gradient, functionally import and export molecules across membrane, required energy
Term
Uniporter
Definition
One molecule going in or out
Term
Symporter
Definition
Two coming in or two going out
Term
Antiporter
Definition
Two molecules, one goes in and one comes out
Term
Simple transporter (uni, sym)
Definition
Just goes in/ just goes out, energy dependent, uses protein motive force
Term
Group transporter
Definition
Chemical modification of product going in,phosphotransferase system
Term
ABC transporter
Definition
ATP binding class, hydrolyzing ATP
Term
Protein export
Definition
Transporters function to bring something in
Term
Cell Wall
Definition
Lots of transport of different compounds and molecules, highly charged, significant gradient
Term
Plasmolysis
Definition
Comes from side cell wall with water chains
Term
Peptidoglycan
Definition
The cell wall in bacteria cells, sugar molecules and amino acids
Term
Gram-Pos
Definition
Typically have a single, thick layer of peptidoglycan
Term
Gram-Neg
Definition
Complex cell wall, which consists of a outer membrane, and a thin layer of peptidoglycan and an inner membrane
Term
X-axis
Definition
Glycosidic bonds
Term
Y-axis
Definition
Peptide bonds
Term
Teichoic acid
Definition
Covalently bonded to NAM, neg a charges of the phosphate contributes to overall neg charge of the cell wall
Term
Periplasmic place
Definition
The space in gram negative that surrounds the peptidoglycan, cellular envelope, contains important proteins
Term
LPS
Definition
Responsible for the core, and O-polysaccharide, very antigenic
Term
Outer membrane
Definition
Lipid bilayer is composed of LPS layer and a typical inner phospholipid bilayer
Term
Porins
Definition
Span across the whole membrane, more nonspecific than transporters
Term
Bacillus
Definition
Anthracis/Cereus/Subtilis
Term
Clostridium
Definition
Tetani/Botulinum
Term
Capsules and slime
Definition
Sticky secretion on bacterial surface used as protection
Term
Fimbriae and pili
Definition
Attachment, conjuction, motility
Term
Subcellular Storage Components
Definition
Carbon storage goes to energy
Term
Magnetosomes
Definition
Bacteria are able to align with magnetic fields
Term
Gas Vessels
Definition
Visualized of blooms, as they feel the sun they float to the surface
Term
Endospores
Definition
Formed during process called sporulation under unfavorable condition
Term
Dipicolinic acid
Definition
Found only in spores, binds to H2O to dehydrate spore
Term
SASPs
Definition
Small acid soluble proteins, tightly bind DNA, carbon and energy source
Term
Sporulation
Definition
Occurs only with environmental stress
Term
Chemotaxis
Definition
Response to chemicals
Term
Quorum sensing
Definition
Form of specialization, senses the other "siblings" in area to start producing the effect
Term
Brownian
Definition
Flows from high to low density
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