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the study of living things that are generally too small to be seen without a microscope |
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conversion of light to energy |
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makes use of microbial metabolism to generate industrial or consumer products |
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manipulation of bacterial genetics |
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use of microbes to restore stability to the environment |
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simple; single celled; lack organelles |
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complex; single or mulit celled; contain organelles |
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microbe acquires life sustaining nutrients from the host, causing damage to the host |
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microbe and host acquire life-sustaining nutrients from one another, each bebfiting the other |
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first to describe the cells as the "units of life" by observing tree bark and plants with magnifying lenses |
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invented the first single-lens microscope; described microorganisms for the first time |
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living things arise from non-living or decomposing matter due to an unseen force |
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first to disprove spontaneus generation |
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said that air is the source of microbes; repeated cycles of heat kills microbes; devised the germ theory of disease |
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theory that microorganism cause disease |
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first to definitively link bacteria to a particular disease |
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1. organism is present in all cases of the disease 2. organism can be cultured 3. organism must be able to produce infection even after several generations of culture 4. organism must be received from an infected animal and cultured again |
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introduced asceptic techniques to hospitals to reduce the number of microorganisms, and therefore, infection |
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Explain the steps of the scientific method. |
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Definition
Make an observation. Formulate a hypothesis as a possible explanantion for the observation, and it must be testable. Experiment to test your hypothesis. Make a conclusion on how correct or incorrect your hypothesis was based on your experiments. |
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when ample data exist to support a given hypothesis it becomes a ___ |
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When enough data exists to support a given theory it may become a ___ |
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the science of naming and classifying living things so that they may be easily identifies |
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systematic arrangement of organisms |
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using traits to recognize an organism |
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List the domains and kingdoms of the Whittaker System |
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Definition
2 domains - eukaryotes and prokaryotes; 5 kingdoms - animals, monera, protists, fungi, and plants |
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List the domains and kingdoms of the Woese-Fox System |
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Definition
3 Domains - eukarya, bacteria, and archaea; 7 Kingdoms - animals, plants, archaea, bacteria, fungi, algae, protozoa |
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the process through which organisms change over time as a result of changes at the DNA level |
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changes that support survival are retained and those that do not are lost |
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the measure of the relatedness of two or more species |
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common form of energy storage and strcutural support |
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not soulble in polar solvents; "fats" |
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define a living organism; made of amino acids joined by peptide bonds |
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"language of life"; DNA and RNA; 5 carbon sugar, phosphate, and nitrogen base |
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List examples of purines and pyridimines. |
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Definition
purines- (single ring)adenine and guanine pyridimines- (double ring) cytosine and thymine/uracil |
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Term
List three ways prokaryotes and eukaryotes differ. |
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Definition
1. the way in which their DNA is packaged (nucleoid in prokaryote not nucleus, and coil around protein, but not histone) 2. composition of the cell wall (prokaryotes is peptidogycan, eukaryotes is polysaccharides) 3. internal organization (prokaryotes don't have membrane-bound organelles) |
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How is a scientific name written? |
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Definition
Genus species (in italics or underlined) |
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What are the 8 taxa from most general to most specific? |
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Definition
domain, kingdom, phylum, class, order, family, genus, species |
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There are 6 groups of cellular microorganisms. which are prokaryotic and which are eukaryotic? |
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Definition
prokaryotic - bacteria and archaea eukaryotic - helminth, fungi, protist, algae |
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Why are viruses neither prokaryotic nor eukaryotic? |
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Definition
because they aren't living cells |
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Can you ever truly accept a hypothosis as fact? |
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Definition
No, because it can never be proved as absolute. New things can always be brought into account and studied. |
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Term
What happens when a flagella rotates clockwise? counterclockwise? |
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Definition
clockwise - causes bacteria to stop and tumble counterclockwise - propels bacteria forward |
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What are the three segments of the flagella, and what are their function? |
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Definition
filament - movement hook - attaches, stabilizes, allows spin basal body - anchors |
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type of linkage that joins saccharides together |
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multiple flagella from one end |
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flagella surrounding the body |
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long rigid projections only found in gram (-) bacteria that allow the transfer of plasmid DNA between certain types of cells |
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short, bristle like projections that mediate attachment |
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protective outer covering present in most bacteria and are composed of polysaccharides and/or proteins |
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what is the difference in the slime layer and capsule glycocalyx? |
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Definition
the slime layer is a thin loose covering that primarliy protects from water and nutrient loss, while the capsule is a thick, dense covering that binds tightly to cells |
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explain the gram staining process |
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Definition
all cells are dyed crystal violet and then given an iodine treatment. only the gram (+) cells hold the violet color, so then the cells are treated with a red counter stain, safarin, which binds to the gram (-) cells |
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not present in all bacteria, but is extremely rigid, made of polysaccahrides and petidoglycan depending on the cell type, and prevents cell bursting due to osmotic pressure |
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only present in gram (-) cells and is composed of LPS outside and phospholipds inside |
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functions of the cell membrane |
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Definition
metabolism, transport, and secretion |
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why is the DNA tightly coiled? |
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Definition
to allow for storage within the cell, otherwise it would be too big |
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not present in all bacteria, extra chromosomal, non essential DNA |
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measure size of ribosomes |
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Definition
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What are the structural differences in a gram (-) and gram (+) cell? |
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Definition
gram (-) has an outer membrane and thinner cell wall |
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What is the primary component of bacterial cell walls? |
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Definition
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Why are gram (-) cells more difficult to kill? |
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Definition
because they have an outer membrane |
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What component of gram (-) cells causes a strong immune response? |
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Definition
the LPS (lipopolysaccharide) in the outer membrane |
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Term
What types of moelcules can pass freely through a cell membrane? |
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Definition
water, and small uncharged molecules |
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Term
what are the three types of bacterial shapes? describe each |
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Definition
cocci - spherically shaped bacilli - cyclindrically shaped spiral forms - thin, spiral shaped |
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Term
3 types of bacilli shapes |
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Definition
coccobacillus - short and plump vibrio - gently curved filamentous - long, thread like |
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differences in the size and shape of bacteria of the same species usually due to slight differences in the cell wall |
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Definition
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how can cocci and bacilli be arranged? |
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Definition
cocci - single, diplo, tetrad, sarcinae, chains, irregular clusters bacilli - single, diplo, strepto, palisades |
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Term
what genuses can form endospores? |
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Definition
Bacillus, clostridium, sporosarcina |
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Term
how are endospores formed and what are their function? |
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Definition
they are formed through sporulation, which deposits a thick protein coat around a chromosome and minimal amount of cytoplasm, which allows the cell to survive harsh conditions |
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Term
process of spore formation |
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Definition
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the breaking of dormancy and return to the vegetative state in bacteria (in the presence of water and at least one other agent) |
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What characteristics are used to classify bacteria? |
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Definition
shape, arrangement, motility, growth characteristics, habitat, and genetics |
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What types of bacteria are phtosynthetic? |
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Definition
cyanobacteria and green and purple sulfur bacteria |
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Term
What is unique about mycoplasmas? |
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Definition
they have sterols in their cell membrane |
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Term
how do archaea and bacteria differ? |
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Definition
the cell wall of archaea contains hydrocarbons rather than lipids |
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What three structures are present in all cells? |
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Definition
cell membrane, ribosomes, and chromosomes |
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Term
Which eukaryotes are unicellular? multicellular? or both? |
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Definition
protozoa are always unicellular, helminths are always multicellular, and fungi and algae can be both |
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Term
archaea that live in extreme heat |
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arcahae that live in extreme salt concentrations |
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archaea that convert C)2 and H2 to methane gas |
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Definition
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archaea that live in extreme cold |
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Definition
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contain enzymes and electron carriers that are responsible for the last steps of aerobic respiration |
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Definition
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long, threadlike cells common to filamentous fungi |
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Definition
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Term
fungi which can exist as mold/hyphal/filamentous form or as yeast. |
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Fungi way of nutrition in which they obtain by digesting organic compounds |
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require nutrients from dead matter |
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Definition
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Term
colonies of filamentous fungi that appear "hairy" |
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Definition
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Term
three parts of sporulation |
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Definition
stalk - sporangiophore bud - sporangium spores - sporangiospores |
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Term
describe the different types of fungal morphology |
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Definition
yeasts - round or oval, asexual hyphae - long, threadlike dimorphic - can be any |
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Term
how do fungi obtain their nutrition? |
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Definition
all are heterotrophs, most are sabrobes, some are parasites, and few are obligate parasites (they can release enzymes into the enviroment to breakdown certain organic material that the cells can then absorb and utilize as nutrients) |
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Definition
spore formation fragmentation |
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what are the two types of fungi? |
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how does yeast reproduce? |
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Term
part of protozoa cytoplasm that deals with movement and feeding |
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Definition
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part of protozoa cytoplasm that contains nucelus, mitochondria, vacuoles, etc. |
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the "mouth" of a protozoa |
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describe the classes of protozoa |
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Definition
mastigiophora - flagella, divide by longitudinal division sarcodina - amoebas, divide by fission ciliophora - cilia, divide by fission
all can form cysts |
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Term
what are the differences between cestodes, trematodes, and nematodes? |
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Definition
cestodes and trematodes are flatworms, while nematodes are round worms. all cestodes are hermaphodites, while no nematodes are hermaphodites, and trematodes can be either or. |
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Term
what are the major capsid arrangements of the animal viruses? |
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Definition
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what characteristics of the viruses are used to classify them? |
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Definition
structure, chemical composition, and sequence |
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Term
describe the life cycle of the animal virus. the bacteriophage. what are the major differences? |
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Definition
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What structures are present in both eukaryotes and prokaryotes? how do they differ? |
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Definition
cell wall, cell membrane, ribomoes, glycocalyx, chromosomes, flagella |
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Term
identical subunits that combine to form the capsid |
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Definition
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Term
allows visualization of the outline of a virus and its surface structure |
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Definition
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allows visualization of internal structure of viruses |
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Definition
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Term
particles of metal are used to cover the virus in order to enhance the shape and surface features |
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present in all viruses; protein shell |
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Definition
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Term
steps in replication of animal viruses |
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Definition
absorbtion, penetration, uncoating, synthesis, assembly, release |
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Term
completely assembled virus particle ready for release |
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Definition
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Term
multiple host cells combine into a single cell with multiple nuclei |
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virus is carried within a host cell for extended periods of time; will cause progressive damage to the cell, but will not kill the cell |
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insert into the host's DNA in a manner that promotes development of cancer; ex. epstein-barr, burkitt's lymphoma, HPV, cervical cancer |
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Definition
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stage present in some bacteriophage replication cycles; viral DNA is inserted into the host's DNA prior to replication; viral DNA remains inactive for a period of time; viral DNA is replicated with host DNA and passed on to daughter cells; allows virus to spread without killing the cell; makes host more pathogenic because inactive viral DNA often encodes a toxin (diptheria, cholera, botulism) |
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smaller than viruses; contain protein but no nucleic acid; ex. mad cow disease |
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virus-like agents; smaller than viruses; infect plants; composed only of RNA (no capsid or envelope) |
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Definition
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virus-induced damage to a cell that alters its mircoscopic appearance; may include swelling and/or the development of inclusion bodies |
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gylcoproteins that protrude from the enevlope of a virus and serve to recognize and attach the virus to the host cell |
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