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Test # 1
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52
Microbiology
Undergraduate 2
09/14/2009

Additional Microbiology Flashcards

 


 

Cards

Term
Legionellosis
Definition

Legionella pneumophila

treatment: Erythromycin

Term
Toxic Shock Syndrome
Definition
Staphylococcus aureus
Term
San Francisco Sourdough Bread
Definition
Candida milleri and Lactobacillus sanfrancisensis makes bread 10times more acidic than normal yeast bread.
Term
Pseudonmonas syringae
Definition
Discovered by Lindow, a bacteria found on strawberries that freeze them.
Term
Lyme disease
Definition

Borrelia burgdorferi

Treatment: Amoxicillin and Doxycycline

Term
Eupulopiscium fishelsoni
Definition
Largest bacteria found to date...
Term
Thiomargarita namibiensis
Definition
Spherical bacteria that is 100x larger than E. fishelsoni
Term
Strain 121
Definition
Bacteria that grows at 121, Archaea.
Term
Spheres-coccus(singular), cocci-plural
Definition
Ex: Neisseria(pairs of cocci, Gram neg.)
Term
Streptococcus(chains of cocci)-Gram positive
Definition
Term

Stapphylococcus

Micrococcus (gram positive)

Definition

Grape-like cluster of cocci

Packets of 8

Term
Bacillus
Definition
RODS
Term
Lactobacillus
Definition
Gram positive
Term

Escherichia coli

Psuedomonas

Definition
Gram neg.
Term
Coccobacillus
Definition
Inbetween rods and cocci
Term
Curved rods
Definition
Spirillum and Vibrio (ridge walled spiral)
Term
Spirochaetes
Definition
are flexible
Term
Treponema
Definition

All Gram negative

Curved rods

Term
Flectobacillus
Definition
Horseshoe shaped
Term
Azotobacter, Mycoplasma
Definition
No cell wall
Term
Escherichia coli
Definition

Flagella: Peritrichous(all around)

Gram: Negative

lifestyle: Lives in soil,water, colon

Temp:37C

Grow with or with O2-Facilitative anaerobe

Circular chromosome

Inform.: Can't bve viewed on high mag. because of low resolution

Most dangerous e.coli is 0157:H7

Term
Resolution
Definition
Distance an object can be viewed and still be distinguished.
Term
Staining
Definition
Cells are dead
Term
Wet mounts
Definition
Observing living cells
Term
Gram stain most important stain
Definition
Because it differentiates
Term
Gram positive
Definition
Purple, very little lipid, small holes so dye stayes in cell
Term
Gram negative
Definition
Pink, lots of lipid, big holes so dye complex comes out--> no color, then counterstains pink with Safranin
Term
Bright field mircroscopy
Definition

Image mag. 1000X-2000X. Limited by resolving power.

 

The shorter the wavelength, the greater the RP

Term
Phase contrast(PC)
Definition
Increased contrast with environment
Term
Dark field
Definition
Uses special condenser that forms a hollow cone of light(used to view surface structures). Used for living cells that are difficult to stain and too small to be seen by PC and BF.
Term
Fluorescence microscopy
Definition
Uses DF and shorter wave lengths, those in blue and uv regions to make speciment fluoresce. Speciment treated with fluorescent dyes, so that it emits light at longer w.l. which can be visualized by FM. Has UV source and filter to protect user.
Term
Electron Micro.
Definition
Uses electrons instead of light. Electrons have w.l. 100,000times shorter than light waves.
Term

Light microscope

human eye

Electron microscope

 

Definition

Resolving Power

.2micrometers

.2milimeters

.2nm

Term
Shakespear
Definition
1564-1616
Term
Bacillus=Rods
Definition

Streptobacillus-chains of rods

Lactobacillus

Clostridium

Term
Spiral shaped
Definition

Treponema pallidum(causes syphilis)

Rhodospirllum-Photo. spirillum

Term
Pseudomonas aeruginosa
Definition

Flagella:motile with polar flagella

Temp: 37C(like e.coli)

Gram:negative(like e.coli)

Shape:Rod (like e.coli)

Circular chrom.(like e.coli)

lifestyle:soil,water,CF lung(opportunitic pathogen)

Strict aerobe

Resistant to antibiotics

Bioflims

Term
Protoplast
Definition
Bacterial cell which the wall has been removed
Term
L-forms
Definition
Lost abilit to form a cell wall
Term
Endospores
Definition
Found in bacillus, clostridium, sporolactobacillus, and thermoactinomycetes
Term
Streptococcus pyogenes
Definition

Gram:Positive

Temp:37C (like e.coli, and P.aruginosa)

Catalase neg.

Peroxidase positive

Causes strep throat,rheumatic fever,childbed fever

 

 

Term
Strapylococcus aureus
Definition

Gram:positive

Heat stable enterotoxin

Causes:TSS, food intoxication from ham, etc.

Facilitative anaerobe

measures: 1-2micrometer

Term
Sterilize
Definition
To make free from all bacteria and other organisms
Term
Disinfect
Definition
to cleanse so as to destroy or prevent the growth of disease-carrying microorganisms
Term
Antiseptic
Definition
An antiseptic is a substance which inhibits the growth and development of microorganisms.For application to skin, mucous membranes, and inanimate objects, although a formal definition includes agents which are used internally, such as the urinary tract antiseptics.
Term
Sanitize
Definition
to disinfect
Term
Pasteurization
Definition
Killing of pathogens, not bacteria or viruses.
Term
Bactericide
Definition
Substance the kills bacteria and ideally nothing else. Such as antiseptic, disinfectant, and antibiotics
Term
Ways to kill bacteria
Definition
  • Moist heat-Autoclave at 121, with 15Ibs of steam per sq. in NOT air.
  • Flash Pasteurization-Heat at 72C for 15secs, 1-3mins @ 140-150C
  • Tyndallization-3times at 100C with incuation at 37
  • Milliipore filter of por size .2micrometer

 

Term
 5 ways antibiotics work
Definition
  • Inhibit cell wall synthesis-Penicillin
  • Inhibit protein synthesis-Tetracycline and Erythromycin
  • Inhibits metabolic function- sulfanilamide
  • Inhibits cell membrane function-polymycin
  • Inhibit nucleic acid synthesis
  • DNA(quinolone)
  • RNA(rifampicin)
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