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Term 1, Biochem Lecture #7
Introduction to Enzymatics
18
Medical
Graduate
09/11/2009

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Term
Holoenzyme
Definition
An apoenzyme + its cofactor
Term
Kcat
Definition
The catalytic efficiency constant for an enzymatic rxn. It is the product formation per enzyme molecule/second
Term
Sources of enzymatic deficiency for a metabolic rxn
Definition
low concentrations of the enzyme, enzyme malfunctioning, genetic defects, nutritional deficits, toxins
Term
How to calculate the rate and velocity of an enzyme
Definition
Keep the [enzyme] constant while manipulating other parameters of the rxn
Term
Prosthetic group
Definition
A tightly bound coenzyme
Term
Covalent catalysis
Definition
This is a transient formation of a covalent catalyst-substrate bond, often performed by Serine
Term
NAD+ and CoA are examples of this
Definition
These are two common examples of coenzymes or cofactors
Term
FAD and Biotin are two examples of this
Definition
These are two common examples of prosthetic groups
Term
Two groups of enzymes that use metals as cofactors
Definition
Metal-cativated enzymes and metallo enzymes
Term
Triad of serine
Definition
Histidine, Arginine and Serine are grouped into this
Term
Six major enzyme groups
Definition
1. Oxidation-Reduction 2. Hydrolases 3. Transferases 4. Lyases/Synthases 5. Isomerases 6. Ligases/Synthetases
Term
This is the function of lyases
Definition
This enzyme has the general function of eliminating leaving groups on a substrate to form a double bond
Term
Main difference between synthetases and synthases
Definition
Synthases do not utilize NTPs for their rxn while synthetases do
Term
Function of Transferases
Definition
Enzymes that catalyze the transfer of functional groups containing carbon, nitrogen and phosphate
Term
Protein kinases and hexokinases phosphorylate these, respectively
Definition
-OH Groups on amino acid side chains and glucose are phosphorylated by these two enzymes
Term
Expressing the rate of an enzyme
Definition
The amount of product (grams)/min
Term
Exressing the velocity of an enzyme
Definition
[product] / min
Term
Cosubstrate
Definition
Coenzymes/Cofactors that only transiently associate with the enzyme
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