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Systems Physiology
Exam 1 - DNA
30
Biology
Undergraduate 3
02/15/2006

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Term
Genes are carried on...
Definition
Chromosomes
Term
Chromosomes contain...
Definition
DNA and Protein
Term
What is so special about DNA that it can hold information?
Definition
X-ray diffraction showed the double helix, which gives potential for replication and information storing
Term
What holds DNA strands together?
Definition
Hydrogen bonds between the bases (A, T, G,C) on the different strands
Term
What are the ends of DNA strands?
Definition
the 3' hydroxyl and the 5' phosphate
Term
How many bases per helical turn are in DNA?
Definition
10 bases
Term
DNA strands run __________ to each other
Definition
Antiparallel, that is, polarities are reversed
Term
The complete set of information in an organism's DNA is called its...
Definition
Genome
Term
Each human cell contains how many meters of DNA?
Definition
2 m
Term
How large is the Eucaryotic ?cell nucleus
Definition
5 to 8 um
Term
Bacteria have what sort/shape of DNA?
Definition
Single, circular DNA molecule
Term
The complex of DNA and protein is called...
Definition
Chromatin
Term
The only nonhomologous chromosomes in humans are...
Definition
Sex chromosomes in males, Y from father and X from mother
Term
A display of the full set of 46 chromosomes is called the human...
Definition
karyotype
Term
A segment of DNA that contains the instructions for making a certain protein is a...
Definition
Gene
Term
What is the nuclear envelope?
Definition
Two concentric membranes punctured by pores which actively transport into the cytosol
Term
What's up with the nucleolus?
Definition
The most prominent nuclear structure during interphase, it's a bundle of DNA which codes for and synthesizes rRNA, rRNA combines w/ proteins to form ribosomes
Term
The cell's protein-synthesizing machines are called...
Definition
ribosomes
Term
The proteins that bind to the DNA to form eucaryotic chromosomes are called...
Definition
Histones. There are also nonhistone chromosomal proteins
Term
What are nucleosomes?
Definition
The first and most fundamental level of chromatin packing - beads on a string (w/ a ball of histone proteins in the middle and DNA wound around
Term
What helps the 4 histones in the nucleosome core to bind to DNA?
Definition
Positive charges of lysine and arginine attract negative sugar-phosphate backbone
Term
How are the nucleosomes of DNA packed even further?
Definition
the 30-nm fiber, a zig-zag model
Term
The most highly condensed form of interphase chromatin is called...
Definition
heterochromatin. Most of it doesn't contain genes
Term
T/F both X chromosomes in a female can be completely inactivated by heterochromatin formation
Definition
False. One of the X chromosomes is condensed this way in female embryos, so that only the condensed and inactive state is inherited
Term
What are chromatin remodeling complexes?
Definition
Protein machines which hydrolyze ATP to change the structure of nucleosomes, allowing on-demand access to DNA
Term
What do the N-terminus histone tails do?
Definition
They facilitate in allowing access to DNA by attracting proteins to either condense or relax DNA packing. They are modified by enzymes
Term
Which are stronger, G-C base pairs or A-T base pair?
Definition
G-C pairs because they have 3 hydrogen bonds which A-T only has 2
Term
What is complementary to:

5'-GGATTTGTC-3'
Definition
3'-CCTAAACAG-5'
Term
In the DNA of certain bacterial cells, 13% of the nucleotides are adenine, what are the otherS?
Definition
13% T, (74/2) C, (74/2) G
Term
T/F Each eucaryotic chromosome must contain the following DNA sequence elements: multiple origins of replication, two telomers, and one centromere.
Definition
TRUE
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