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| Diverse and made up of multiple interconnected elements |
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| Diverse and made up of multiple interconnected elements and adaptive in that they have the capacity to change and learn from experience |
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Definition
| the amount of resources required to execute a task is proportional to its complexity |
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| the kolmogorov of an object such as a piece of text is a measure of the computational resources needed to specify the object. |
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| Study of the interactions between the components of biological systems, and how these interactions give rise to the function and behavior of that system |
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| Humans are finate (in space and time), and therefore cannot perform all "extended" measurements. Therefore there are things in science that cannot be known be humans |
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| A knockout mouse is a lab mouse that researchers have "knocked out" an existing gene by replacing it disrupting it with a piece of DNA |
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| undifferentiated cells derived from the 5-day preimplantation embryo that can become a widew variety of specialized cell types |
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| Plus-minus selection for homologous recombination |
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Definition
| The plasmid vector is constructed in such manner that only homologous recombination between specified exons of plasmid and ES cells will be allowed to clonally proliferate. |
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| Selection for non-homologous recombination in knockout mice |
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Definition
| Once non-recombinants have been selected against by treatment with neomycin the remaining ES cells are then grown in media contain ganciclovir. Those ES cells that have undergone a non-homologous recombinational event and have therefore incorporated both the neomycin resistance and the ganciclovir susceptibility will not grow, |
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| Reinject the blastocysts with the ES cells |
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Definition
| The remaining ES cells are those have undergone the proper homologous recombination event required to knockout the targeted gene, and are then allowed to proliferate to create a clonal population of single allele knockout ES Cells. |
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