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Symbiosis
Exam 2
18
Microbiology
Undergraduate 4
02/16/2010

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Term
2 sets of bacteria that suppress plant disease
Definition
Bacillus and Pseudomonas species
Term
Pseudomonad characteristics (3)
Definition
1. aggressive root colonization
2. induce systemic resistance
3. antibiotic (some controlled by quorum-sensing)
Term
Role of 2,4-diacetylphloroglucinol
Definition
suppress tobacco black root rot
Term
phytophthora life cycle
Definition
sporangium --> zoospore --> cyst --> mycelium --> oospore/chlamydospore/sporangium
Term
ZmA
Definition
Zwittermicin = antibiotic

-blocks step that inhibit cyst from making mycelium
-no ZmA = less biocontrol activity
-add ZmA = restore disease control
Term
problems of initial screen on plants (greenhouse/field efficacy)
Definition
strains inhibit pathogen with antibiotic
Term
problem with initial screen for antibiosis (inhibition of pathogen)
Definition
lack biocontrol efficacy in greenhouse/field
Term
positive effects of PGPR (2)
Definition
1. bioavailability of N,P, Fe

2. plant hormone manipulation
(increase auxin,cytokinin,gibberellins/decrease ethylene)
Term
Azospirillum brasilense
Definition
alpha-proteobacterium that fixes N, motile, forms enlarged cyst-like structures, produces IAA, PGPR activity may relate to N-fixation and IAA
Term
Pros of N-fixation (2)
Definition
1. fixes N for plants N-limited

2. reports of increased yields
Term
Cons of N fixation (4)
Definition
1. promotes growth in rich/fertilized soil

2. can't account for increase yield

3. doesn't excrete much ammonia

4. regulation = only fixes N when it needs it
Term
3 pathways to NO
Definition
Aerobic denitrification, heterotrophic nitrification, and NO synthase (NOS)
Term
aerobic denitrification
Definition
nap genes responsible to make NO

NO3- to NO2- to NO + N2O
Term
heterotrophic nitrification
Definition
NH4+ oxidized to NO3- with NO produced at intermediate stag
Term
NO synthase (NOS)
Definition
arginine + O2 = L-citrulline + NO
Term
LR vs AR
Definition
LR- lateral roots that extend horizontally from primary root

AR-adventitious root that are located in places roots normally don't exist
Term
Agrobacteria
Definition
induces crown gall (not source of all galls), doesn't infect monocots, wide host range
Term
Agrobacterium basics
Definition
present in soil, plants must be wounded for infection, galls due to hormone overproduction, galls produce opines that agro can use
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