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Details

Summary of Test Performed
Descriptions of Lab Test
45
Microbiology
Undergraduate 1
11/16/2011

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Cards

Term
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Definition
NEGATIVE. Fast fermenters turn from dark green to black. Slow fermenters turn are pink/maroon.
Term
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Definition
Positive. No growth white/colorless
Term
EMB
Definition
Uses Esosin Methylene blue media.It is select for gram NEGATIVE BACTERIA and DIFFERENTIAL for lactose fermenters.
Term
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Definition
Growth is gram NEGATIVE (fermenter) salmon color. No growth is gram POSITIVE (non-fermenters) blue-bluegreen.
Term
HE
Definition
Uses Hektoen enteric agar media. Selective for gram NEGATIVE BACTERIA
DIFFERENTIAL for lactose fermenters
Term
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Definition
Growth gram POSITIVE. No growth gram NEGATIVE.
Term
PEA
Definition
Uses Phenylethyl alcohol agar media. Select for Staphylococcus DIFFERENTIAL for mannitol fermenters.
Term
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Definition
Growth is gram POSITIVE (fermenter) YELLOW. No Growth is gram NEGATIVE (non-fermenter) RED.
Term
MS
Definition
Uses Mannitol salt agar media and phenol red reagent. Select for Staphylococcus DIFFERENTIAL for mannitol fermenters.
Term
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Definition
No clear zone has NEGATIVE results. Clear zone around bacterial growth has POSITIVE results.
Term
Caseinase Production
Definition
Uses Milk agar media. It also detects if bacteria can break down casein (C.violaceum).
Term
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Definition
Gram POSITIVE as evidenced by the liquidation of the media.
Term
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Definition
Gram NEGATIVE as evidenced by the solidity of the media.
Term
Gelatinase Production
Definition
Uses Nutrient gelatin and can detect if bacteria can break down gelatin (S.aureus, L. monocytogenes).
Term
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Definition
Salmonella typhimurium left is negative no clear zone. Pseudomonas aeruginosa right is positive clear zone surrounding the growth.
Term
Lipase Production
Definition
Uses Tributyrin agar media and detects if bacteria can break down tributyrin.
Term
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Definition
Purple color after iodine added NEGATIVE results. Clear zone around bacterial growth when iodine is added POSITIVE results.
Term
Amylase Production
Definition
Uses Starch agar media and iodine reagent. It detects if bacteria can break down carbs (C. diphtheriae, C. perfringes, B. anthracis).
Term
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Definition
Red is NEGATIVE. Yellow (with full small tube inside) POSITVE. Yellow (with tube slightly not full inside) Fermentation and gas have been produced.
Term
Carb Fermentation
Definition
Uses Durham tubes, Fermentation broth=carb (glucose,lactose,sucrose). It uses Phenol reb reagent and detects if bacteria can ferment different carbs.
Term
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Definition
RED after adding reagents A&B POSITIVE. If added zinc COLORLESS POSITIVE. Remained RED after adding zinc NEGATIVE.
Term
Nitrate test
Definition
Uses Nitrate reagent A&B and Zinc. It identifies the way nitrate is reduced in certain bacteria.
Term
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Definition
Colorless NEGATIVE. PURPLE POSITIVE.
Term
Oxidase Test
Definition
Media/Reagent TMPD. It detects cytochrome c oxidase activity (All Enterobacteriacease are NEGATIVE).
Term
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Definition
No bubbling POSITIVE. Bubbling NEGATIVE.
Term
Catalase Test
Definition
Uses Hydrogen peroxide reagent. Test for Catalase Production.
Term
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Definition
NEGATIVE YELLOW. POSITIVE RED.
Term
Indole Test
Definition
Uses Tryptone broth and Kovac’s reagent. Test for ability to break down Tryptophan and yield indole.
Term
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Definition
RED POSITIVE. YELLOW NEGATIVE.
Term
Methyl Red Test
Definition
Uses MR-VP broth and Methyl Red reagent. It has the ability to ferment glucose and form acids.
Term
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Definition
No color change NEGATIVE. Deep PINK POSITIVE.
Term
Voges-Proskauuer Test
Definition
Uses MR-VP broth and Barritt’s reagent. It detects presence of acetoin.
Term
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Definition
Turns from green to royal blue POSITIVE.
Term
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Definition
No color change, remains green NEGATIVE.
Term
Citrate Test
Definition
Uses Simmon’s citrate agar media and bromthymol blue reagent. It detects the ability to utilize citrate acid as sole source of carbon.
Term
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Definition
dark green POSITIVE.
Term
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Definition
No color change NEGATIVE.
Term
Phenylalanine Deamination
Definition
Uses Phenylalanine slantsand 10% ferric chloride. It test for Phenylalanine Deamination – most bacter that produce this are pathogenic (ie Proteus, Morganella).
Term
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Definition
PURPLE POSITIVE
Term
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Definition
No color change YELLOW NEGATIVE.
Term
Lysine/Ornithine Decarboxylation Test
Definition
Uses Lysine and Ornithine broth, mineral oil, Bromcresol purple. Test for lysine and ornithine decarboxylase (one way to overcome IMViC tests limit).
Term
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Definition
No color change is NEGATIVE. Black precipitate POSITIVE.
Term
Hydrogen Sulfide Test
Definition
Uses Peptone iron agar media and deep containing ferrous sulfate. It detects hydrogen sulfide gas production.
Term
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Definition
Orange and yellow are NEGATIVE. Pink is POSITIVE.
Term
Urease Test
Definition
Uses Urea broth and phenol red. It detects urease production and is useful in distinguishing Proteus.
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