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study for lab quiz 1
study for lab quiz 1
56
Microbiology
Undergraduate 1
09/12/2012

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Cards

Term
what is the commonly used stain?
Definition
gram stain
Term
growth media is used to culture what
Definition
microbes
Term
what 3 things do microbes need to grow?
Definition
nutrients, moisture, and other chemicals
Term
the shape and arrangement of the cells with their staining properties are used for what ?
Definition
classifying and preliminary identifying clinical isolates
Term
what are the 3 stain?
Definition
gram stain, acid-fast stain, and endospore stain
Term
what are the two main type of media used?
Definition
artificial culture media and tissue culture media
Term
what media is made of agar or broth which contain the necessary nutrients to grow a variety of bacteria and fungi?
Definition
artificial culture media
Term
nutrient included in artificial media culture are what 4 things?
Definition
carbohydrates (glucose, lactose), amino acids, growth factors, and buffers
Term
what is an example of the type of bacteria that will not grow on artificial culture?
Definition
chlamydiae and rickettsiae
Term
what is the genus name of chlamydiae?
Definition
chlamydia causative agent of the STD chlamydia
Term
what is the genus name for rickettsiae?
Definition
rickettsia causative agent of Rocky Mountain Spotted Fever
Term
what media contains living cells/tissue which act like "host" cells in order to grow and study viruses and other obligate intracellular organisms?
Definition
cell or tissue culture media
Term
chlamydia and rickettsia bacteria are both __________ _________ that must have host cells to grow
Definition
intracellular pathogens
Term
what is a liquid media that becomes turbid as bacteria grow?
Definition
broths
Term
_________ refers to the change that occurs in broth as bacteria grow
Definition
turbidity
Term
true or false
the more bacteria grow the more they increase the cloudiness of the broth, changing it form the original clear to a "dirty" appearance
Definition
true
Term
example to turbidity is going to the swimming pool to ________ __________
Definition
jordan lake
Term
what is a texture much like firm gelatin, but made of seaweed?
Definition
agar
Term
when agar is poured into a test tube and allowed to solidify so that the agar surface in the tst tube is slanted it is called?
Definition
agar slant or "slant"
Term
agar slant or "slant" is placed in storage for how long?
Definition
4-6 weeks
Term
whan agar is poured into a petri dish and allowed to solidify it is called?
Definition
agar plates
Term
what produces a relatively large flat surface for bacterial growth and isolation?
Definition
agar plates
Term
what are the 3 different type of agar and broth?
Definition
non-selective, differential, and selective
Term
the media that will support the growth of many (but not all) bacteria of clinical interest is called what variety of agar
Definition
non-selective
Term
sheep's blood agar (SBA) is what variety of agar?
Definition
non-selective and differential
Term
trypticase Soy Agar (TSA) is what variety of agar?
Definition
non-selective
Term
chocolate agar is what variety of agar?
Definition
non-selective
Term
nutrients agar (NA) is what variety of agar?
Definition
non-selective
Term
blood agar is made by adding 5% sheep's blood to a complex nutrient ________ _________ _________media
Definition
trypticase soy agar
Term
in blood agar the ______ ________ cells remain intact and the medium resembles fresh blood
Definition
red blood
Term
blood agar can be differential by means of what?
Definition
hemolysis
Term
what are the 3 hemolysis used in blood agar?
Definition
gamma hemolysis, beta hemolysis, and alpha hemolysis
Term
what hemolysis indicates no lysis of RBC's
Definition
gamma hemolysis
Term
what hemolysis has bacteria grow on blood agar without disturbing the red blood cell- leaving the RBCs intact?
Definition
gamma hemolysis
Term
what hemolysis causes complete breakdown of RBC?
Definition
beta hemolysis
Term
what hemolysis causes clearing of the "blood" around the colonies?
Definition
beta hemolysis
Term
what hemolysis cause partial breakdown of RBC?
Definition
alpha hemolysis
Term
what hemolysis has bacteria lyse the cells but do not completely break down the hemolysis, which leaves a greenish hue (methemoglobin) in the medium?
Definition
alpha hemolysis
Term
what not only helps to support the growth of the bacteria, but it also can serve to provide clues to the identity of the bacteria growing?
Definition
SBA
sheep blood agarr
Term
what variety of agar has media in which some metabolic activity of an organism can be detected by inspection of the growth of the organism on the medium?
Definition
differential media
Term
differential media is often accomplished by observing changes in the color of ____ indicator
Definition
ph
Term
triple sugar iron agar (TSI) is an example of what type of media?
Definition
differential media
Term
mannito salt agar (MSA) is an example of what type of media?
Definition
differential media
Term
eosin methylene blue agar is an example of what type of media?
Definition
differential media
Term
MacConkey agar is an example of what type of media?
Definition
differential media
Term
other non-colorimetric indicators include _______ __________
Definition
hemolysis patterns
Term
in the broadest sense, all media are __________ , in that there is no universal medium on which all species of bacteria can grow
Definition
selective media
Term
what type of media is restricted to situation where an ingredient is added which allows the growth of a particular organism, while inhibiting to a considerable extent the growth of other organisms which might be found in the same environment
Definition
selective media
Term
normal flora verses pathogen is an example of what type of media
Definition
selective media
Term
what selective media agar contains bile salts and crystal violet dye, which inhibits Gram-positive organism?
Definition
MacConkey agar
Term
what selective media agar contain small quantities of the antimictobial agents vancomycin, colistin, and nystatin, inhibits the common mictobiota of the genital area, while selection for Neisseria?
Definition
thayer-Martin agar
Term
what selective media agar contain 7.5% salt, which inhibits many common microorganisms, but allows for the growth of Staphylococcus species?
Definition
mannitol salt agar
Term
what is SALT-TOLERANT?
Definition
staph
Term
inhibitors in selective media are (1)_________ in low concentration,(2) ______ _______, high (3)______ concentration and other substances such as (4)__________ __________ are often used to inhibit and/or encourage growth
Definition
(1) dyes
(2) bile salts
(3)NaCl
(4)phenylethyl alcohol
Term
many but not all selective media are also _________, often allowing for discrimination between differing species of one genus
Definition
differential
Term
what only allows for growth of Staphylococci species, and uses pH indicator and the sugar alcohol Mannitol to distinguish between S. aureus and S. epidermidis species
Definition
MSA
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