Term
| What is the definition of sterile? |
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Definition
| Free of all microorganisms and their spores including viruses |
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Term
| What is the difference between universal precautions and standard precautions? |
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Definition
| Universal precautions are applied to specimens known to transmit specific disease. Standard precautions are applied to all specimens as if they can transmit disease |
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Term
| What is the purpose of sterilization and disinfection? |
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Definition
| To prevent contamination of laboratory personnel and specimens |
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Term
| T/F. Vegetative forms of bacteria are more resistant to antimicrobics than are bacterial spores. |
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Definition
| False. Bacterial endospores are much more resistant to antimicrobial agents than vegetative forms |
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Term
| What instruments are sterilized using an incinerator? |
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Definition
| On small metal instruments at the bench |
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Term
| Name two disadvantages of the dry heat oven. |
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Definition
1) Less efficient than moist heat (autoclave)
2) Requires a longer exposure time and higher temperatures |
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Term
| What are the proper settings for an autoclave? |
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Definition
1) 121 degrees C
2) 15 PSI
3) 15 minutes |
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Term
| What products are generally sterilized using gamma radiation? |
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Definition
| Mass-produced disposables |
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Term
| What physical method of sterilization is ineffective against viruses? |
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Definition
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Term
| Which of the chemical disinfectants would be good to use on surgical instruments? |
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Definition
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Term
| Which antiseptic would be the first choice for skin disinfection? |
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Definition
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Term
| Which BSL would be most appropriate for working with the Ebola virus? |
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Definition
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Term
| Which class of biological safety cabinet would give the laboratory worker the greatest amount of protection? |
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Definition
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Term
| When is it appropriate to wash your hands while working in the laboratory? |
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Definition
| On a routine basis, upon removal of gloves, and must be done before leaving the lab |
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Term
| What should you use to disinfect your tabletop? |
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Definition
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Term
| Describe in your own words how to clean up a small spill. |
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Definition
1) Cover spill with paper towels
2) Soak with disinfectant (10% bleach)
3) Report accident to an instructor/supervisor
4) Allow to remain undisturbed giving disinfectant time to work
5) Carefully wipe up spillage or breakage
6) Dispose of materials in an autoclave bucket |
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Term
| Describe in your own words how to clean up a large spill. |
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Definition
1) Sprinkle powder absorbent around the spill to contain the spread of the spill
2) Continue to sprinkle the absorbent over the spill until it is congealed or soaked up
3) Pick up as much of the congealed material as possible using one or more of the disposable scoops/scrapers
4) Place all the material and the scoop/scraper in a biohazard bucket
5) Spray the affected area with disinfectant and allow it to stand for ten minutes
6) Wipe up area and place all contaminated materials in a biohazard container
7) Allow disinfectant to air dry |
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Term
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Definition
| The use of physical and/or chemical procedures to completely eliminate or destroy all forms of microbial life |
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Term
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Definition
| A substance that kills all living microorganisms, including spores, both pathogenic and non-pathogenic |
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Term
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Definition
| An agent or environmental condition that inhibits growth or multiplication, but does not necessarily kill them |
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Term
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Definition
| A process that reduces or completely eliminates all pathogenic microorganisms except spores, usually from objects |
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Term
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Definition
| A chemical agent used to carry out disinfection |
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Term
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Definition
| A substance that inhibits or destroys microorganisms. Used specifically for substances applied topically to living tissue |
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Term
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Definition
| Free of contaminating or infectious microorganisms |
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Term
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Definition
| Term used to describe a process devised to render a medical device, instrument, or environmental surface safe to handle |
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Term
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Definition
| Easily altered, decomposed, or destroyed by heat |
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Term
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Definition
| Not easily altered, decomposed, or destroyed by heat |
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Term
| What is the most commonly used physical method for killing and inhibiting microorganisms? |
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Definition
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Term
| What is the settings for a Dry heat oven? |
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Definition
| 160 to 180 degrees C for two hours |
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Term
| What is the usually effective filtration pore size for removing all bacteria? |
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Definition
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Term
| What is the most obvious difference between BSL 1 and BSL 2? |
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Definition
| The use of gloves and biological safety cabinets |
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Term
| What class of biological safety cabinet is the most common in the microbiology laboratory? |
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Definition
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Term
| What is the difference between a chemical indicator and a biological indicator when performing QC of an autoclave? |
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Definition
| Chemical indicators monitors that an item was at least processed through a sterilizing cycle. Biological indicators are a direct measurement of desired outcome (the destruction of microbial life) |
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Term
| Which class of biosafety cabinet does not protect the specimen from contaminates found in the room air? |
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Definition
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