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Sterilization/Media/Cultures/microscope/spc
Micro Lab 1 quiz
55
Microbiology
Undergraduate 2
03/16/2011

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Term
Sterilization
Definition
process in which all life forms are killed or removed from an object or substance.
Term
Disenfection
Definition
Process which renders an article incapable of transmitting disease(pathogens destroyed at least)
Term
Virulence
Definition
the degree of pathogenicity.
Term
Nosocomial Infections
Definition
infection acquired while during hospital care.
Term
Aseptic Technique
Definition
Techniques which exclude potential infective agents from tissues or lab materials. (prevents sepsis /infection)
Term
Degerming
Definition
removal of microbes from a limited area of skin( alcohol /betadine)
Term
Methods of Sterilization
Definition
1. Hot air Oven
2.autoclave
3. Filtration
4. Gas Sterilization
5. Strong Chemical Sterilization
/disenfection
Term
Hot Air Oven
Definition
1. Kills by Protein Oxidation
2. 160-70 C for 1-2 hours
3. For glassware,pippetts,oils,powders
Term
Autoclave
Definition
1. Kills by protein Denaturation
2. Live steam under pressure "heat of condensation to microbial cells.
3. 121 at 15psi for 30 minutes.
4. for instruments,simple media,meatalware.
Term
Filtration
Definition
Hepa Filter.
1. Can Remove all bacteria greater than .3 micrometers
2. some filters have .01 mmbrane pores for viruses.
3. Used for any injectable fluids and hemodialysis fluids. (prevents endotoxin effects.)
Term
Gas Sterilization-Ethylene Oxide Gas
Definition
1. Kills by Alkylation
2. 2-12 hours
3. Plastics,electronics,rubber items.
Term
Gas Sterilization-Chlorine Dioxide
Definition
1.Short lived gas used in closed buildings with Anthrax Spores.
2. used to fumigate
Term
Gas Sterilization-Ultraviolet Light
Definition
1. Used to decrease airborne infection.;
2.High Humidity Limits use.
3. Hospital rooms,nursuries,or rooms, cafeterias.
Term
Strong Chem Ster-Ethyl Alcohol (70%)
Definition
Generally a strong degerming
Term
Strong Chem ster/Disenfection-Plasmas
Definition
1. Electromagnetic exitation of gas
2. Used for surgical instruments used in Arthroscopic or Laparoscopic procedures.

Placed in containers with vacuum,electro mag feild, chms like hydrogen proxide which form plasma.
Term
Strong Chem ster/ Disenfection- Aqueous or Gaseous Formaldehyde-
Definition
1. Works at 25C
2. Used Formaldehyde Crystals which were vaporized to kill Ebola Virus.
Term
What Kills the Ebola Virus?
Definition
Gaseous Formaldehyde/Crystals
Term
What Kills the Ebola Virus?
Definition
Gaseous Formaldehyde/Crystals
Term
What Kills the Ebola Virus?
Definition
Gaseous Formaldehyde/Crystals
Term
What Kills the Ebola Virus?
Definition
Gaseous Formaldehyde/Crystals
Term
Strong Chem Sterl/Disen-Gluteraldehyde(SIDEX)
Definition
Strong disenfectant for surgical instruments/dental tools.
Term
Strong Chem- Amphyl
Definition
Floors Walls and furniture. (hospital use primarily)
Term
Strong Chem Sterl/disen- Bleach
Definition
1.Antiviral oxidizing agent
2. rooms and equipment
3. Used by CDC apartments/hotels/homes etc.
Term
Purpose Of Sterilization
Definition
To prevent contamination of materials,media, and patients by bacteria, protozoa,fungi and viruses.
Term
Media
Definition
Chemicals used for the matinence of life, growth and reproduction of microbes.
Term
In vitro Media
Definition
Media which only contains inanimate materials. Used for Bacteria and Fungi.
Term
Bacteria and Fungi use what kind of media?
Definition
In Vitro Media
Term
In Vivo Media
Definition
Media which living cells (chick embryo,live animal or tissue culture use to live) Used for viruses and Ricikettsia.
Term
What media type is used for Viruses and Ricikettsia?
Definition
In Vivo Media.
Term
What is the universal biological solvent in media?
Definition
H2O
Term
What nutrient compounds are used in all media?
Definition
C,O,N,S,H,P
Term
What are the inorganic nutrients?
Definition
Co2 H2S,NH3 , Phosphates, and sulfates
Term
What are the organic nutrients?
Definition
Sugars, Fatty Acids,Amino Acids, Nucleic Acids.
Term
Growth Factors-
Definition
Essential chemicals which cannot be made by bacteria but are critical to metabolism.
Term
Nutrients
Definition
Compounds containing essential elements (C,O,N,S,H,P) which are oxidized to generate energy (ATP) or reduces to build cellular structures.
Term
Inorganic G-F Growth Factors
Definition
Ca+,Mg++,Si++,Fe++,Co,Na+,K+
Term
Organic G-F Growth Factors
Definition
Vitamins and certain Amino Acids.
Term
Bacteria Require a ________pH.
Definition
Neutral
Term
Fungi prefer an _________pH.
Definition
Acidic
Term
pH of Bacteria Media
Definition
6.8-7.2
Term
pH of Fungal Media
Definition
5.5-6
Term
Synthetic Media
Definition
Media in which the exact chemical formulation is known.
Term
Non-Synthetic Media
Definition
not all chemicals are known(blood, tissues etc.)
Term
Use of media
Definition
purposes of various ingridents to promote growth
Term
general Purpose media
Definition
allows a large number of bacteria to grow.
Term
Differential Media
Definition
Colony Morphology on agar gives clues to ID
Term
Selecive Media
Definition
Only Allows certain bacteria or fungi to grow ( dyes,pH)
Term
Transport Media
Definition
Temporary holding media-preserves bacteria for lab.
Term
Agar melts at
Definition
95C
Term
agar can be poured at
Definition
50C
Term
agar solidifies at
Definition
42C
Term
Ingredients of Nutrient Broth and Nutrient Agar
Definition
1. H2O as biological solvent
2. peptone-protein digest which provides nutrients
3. Beef extract:provides groth factors
4. NaCl:to adjust Osmotic Pressure
5. Agar as solidifying agent.
Term
Enriching Media
Definition
Additives:Serum,yeast extract,blood,liver...etc
Term
Indicators used in Media
Definition
Indicators/dyes used to ID the pH and changes to pH as bacteria metabolize specific media(bromthymol Blue, Phenol Red, Methyl Red.)
Term
Forms of Media Used in Micro 33
Definition
1. broths
2. Slants
3. Plates
4. Fermentation Tubes
5. FTM-Thioglycollate Broth (O2 requirements)
6. stabs
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