Term
|
Definition
Uses two stains; Allows us to differentiate between two organisms |
|
|
Term
Types of differential staining |
|
Definition
Gram Stain, Acid-Fast Stain, Spore Stain |
|
|
Term
|
Definition
Differential staining technique using crystal violet and safranin dies that separate bacteria into two large categories: gram (+) and gram (-). |
|
|
Term
|
Definition
uses a negatively charged stain like nigrosin, which is not taken up by the cytoplasm of negatively charged bacteria. Background is stained, not the bacteria. |
|
|
Term
Bacteria commonly viewed with negative stain |
|
Definition
Streptococcus pneumonia, Klebsiella pneumonia, Clostridium perfringens: bacteria with well-developed capsules |
|
|
Term
|
Definition
The primary dye, malachite green, dies the spore wall, but is washed off the cell wall with a decolorizer. Cell walls are counterstained with safranin. Spores left green. Used with Bacillus and Clostridium. |
|
|
Term
|
Definition
Stains the cells of the genera Mycobacterium and Nocardia. Their cell walls have a waxy lipid substance and do not take water based stains well. The cell is determined to be acid-fast or not if it holds the primary stain. |
|
|
Term
Gram category and morphology of Bacillus |
|
Definition
|
|
Term
Gram category and morphology of Clostridium |
|
Definition
|
|
Term
Gram category and morphology of Staphylococcus |
|
Definition
|
|
Term
Gram category and morphology of Streptococcus |
|
Definition
|
|
Term
Gram category and morphology of Escherichia |
|
Definition
|
|
Term
Gram category and morphology of Klebsiella |
|
Definition
|
|
Term
Gram category and morphology of Mycobacterium |
|
Definition
|
|
Term
Gram category and morphology of Neisseria |
|
Definition
|
|
Term
Gram category and morphology of Pseudomonas |
|
Definition
|
|
Term
Gram category and morphology of Salmonella |
|
Definition
|
|