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closely resembles archaeon ancestor thermophiles/hyperthermophiles includes genus: Sulfolobus includes genus: Thermoproteus |
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thermoacidophiles aerobes chemolithotrophs that oxidize sulfur found in hot springs & soil |
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thermoacidophiles strict anaerobes chemoorganoautotrophs or chemolithoautotrophs oxidize glucose during anaerobic resp. uses SULFUR as final electron acceptor found in hot, aquatic habitats |
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display a variety of metabolic patterns and found in many different environments 5 major physiologic groups including: Methanogens Halobacteria |
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The Methanogens (phylum euryarchaeota) |
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Definition
Include all archaea that generate CH4: methanogenesis Largest group of archaea Strict anaerobes Have unique electron carriers Use CO2 as e- acceptor (reduced to CH4) Varied shapes, cell membranes, cell wall found in sewage, GI tracts, places with lots of organic matter and low O2 Could be a potential source of fuel: CH4 CH4 + IR radiation = greenhouse gas |
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The Halobacteria (phylum Euryarchaeota) |
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Definition
Archaea (not bacteria) Live in high salt concentration >8% Can grow in up to 36% NaCl Some are alkalophilic rods, cocci, pyramids cell wall = glycoprotein-needs salt (-) charges on wall shielded by Na+ Concentrates K+ inside cell (pi balance) needs high K+ for enzymes & ribosomes Aerobic chemoheterotrophs Need lots of growth factors Have: Rhodopsin, Halorhodopsin, & 2 sensory Rhodopsins-control flagella act. (one senses red and one blue) |
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Light-driven proton pump retinal reacts with 2 amino a's in light intense, low O2 environment Reversibly accepts & donates protons Synthesizes ATP |
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Transport pump that uses light E fo ion balance (to concentrate Cl-) |
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Gram-negative nonproteobacteria photosynthesizers |
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Chlorobium Chloroflexus Cyanobacteria Planctomyces Chlamydia Spirochetes (treponema pallidum;borrelia burgdorferi) Bacteriodes |
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gram-negative nonproteobacteria (non-photosynthesizers) |
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Definition
Aquifex Thermatoga Deinococcus |
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relatively primitive oldest branch of bacteria hyperthermophiles aerobic chemolithoautotrophs |
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2nd oldest branch of bacteria hyperthermophiles anaerobic chemoheterotrophs use glycolytic pathway* have an outer sheath-like envelope* |
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Have atypical cell walls: stain gram(+) have thick peptidoglycan layer** (no teichoic acid like gram (+)) have outer membrane (like other gram(-)) resistant to desication & radiation able to repair genome after radiation |
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Chlorobium--Green sulfur bacteria |
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Definition
use anoxygenic (cyclic) PS for ATP (no z pathway) only use exogenous donor for CO2 fixing have chlorosomes* attached to PM to capture light and pass to photosystem |
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Chloroflexus --Green nonsulfur bacteria |
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Definition
photoheterotroph or chemoheterotroph If using CO2 fixation, uses reverse e- flow have small chlorosomes* thermophilic |
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highly variable G+C content (35-71%) chloroplast precursor photoautotrophs>Z pathway, chlorophyll a, 2 photosystems phycobilin* acc. pigments: phycocyanin & phycoerythrin |
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Structures/cell types produced by cyanobacteria |
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Definition
Baeocytes Hormogonia Akinetes Heterocysts Gas vesicles Gliding Motility (slime) |
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Lack peptidoglycan in cell wall* have compartmentalized cell walls* have a stock & holdfast for attachment* can lose stock & holdfast, produce a flagella to become motile swarmer cells |
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Obligate intracellular parasites (like viruses) No peptidoglycan in cell wall* compensate with sterols in membrane* have an outer membrane may be de-evolving / very small genome |
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1)Attachment of EB(infectious) to cell surface, phagocytosis upon attachment 2) EB morphs into RB (not infectious) and replicates by binary fission 3) RB morphs back to EB and cell lyses |
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use Endoflagella* w/an axial filament* contain Treponema Pallidum & Borrelia Burgdorferi |
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Spirochete Causes syphilis* can't be cultured in lab depends on host (no TCA cycle, ox phos) |
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Spirochete causes lyme disease has a linear genome* has at least 17 plasmids that all confer virulence |
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very common mixed acid fermentors* live in oral cavities, GI tract, rumen of ruminants 30% of fecal bacteria |
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purple nonsulfur bacteria rickettsia caulobacter rhizobium agrobacterium tumefaciens |
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purple nonsulfur bacteria |
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Definition
some form cysts do not oxidize sulfur to sulfate can use reverse electron flow perform anoxygenic photosynthesis (1 PS) exist as photoorganoheterotrophs contain bacteriochlorophyll |
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closest relative to mitochondria* obligate intracellular pathogens induce phagocytosis -escapes phagosome no glucose needed-uses host TCA intermediates causes typhus & rocky mtn spotted fever* |
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an alphaproteobacteria (gram - proteobact) |
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Definition
have prostheca*-a stock/extension of cell used for attachment undergoes binary fission with a prostheca have flagella for movement |
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Nitrogen fixers in root nodules Mutualistic with plants |
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Agrobacterium tumefaciens |
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Definition
agent of Crown Gall disease* Ti plasmid has genes for plant hormones & opines |
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Thiobacillus -colorless sulfur bacteria |
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Thiobacillus-colorless sulfur bacteria |
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chemolithoautotrophs oxidize inorganic sulfur increase soil fertility cause acid & metal pollution & pipe corrosion |
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purple sulfur bacteria pseudomonads enteric bacteria |
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use anoxygenic photosynthesis (1 PS) photolithotrophs oxidize H2S to elemental sulfur strict anaerobes* have bacteriochlorophyll |
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Definition
large, diverse group rods w/polar flagella chemoheterotrophs (aerobic or anaerobic resp) |
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practical importance of pseudomonads |
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mineralization (b/d or organic material) experimental subjects (biofilms) animal and plant pathogens food spoilage |
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comma-shaped rods w/polar flagella facultative anaerobes some are bioluminescent pathogens: cholera, gastroenteritis |
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Largest group Proteus, Escherichia, Salmonella, Shigella, Kebsiella, Yersinia, Enterobacter Homogenous phylogenetic group nonmotile or motile by peritrichouse fl. facultative anaerobes Mixed acid fermentation or Butanediol fermentation |
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Bdellovibrio Desulfovibrio |
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Strict anaerobes* reduce sulfur or sulfate important in cycling of sulfur can cause anaerobic corrosion of iron |
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Definition
Rods w/polar flagella parasite of other gram (-) bacteria* not obligate intracellular parasites* alternate between nongrowing predatory phase & intracellular reproductive phase efficient use of nutrients-obtains large amt of nutrients from host |
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Life cycle of Bdellovibrio |
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Definition
Rams into prey-bores hole by rotating Also secretes hydrolytic enzymes grows in periplasmic space of hose steals through a hold in host PM inhibits DNA/PRO synthesis Divides by multiple fission |
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Definition
Most complex behavior & life cycle of all prokaryotes-largest genome chemoheterotrophs-use respiration micropredators/scavengers produce hydrolytic enzymes to lyse prey |
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Life cycle of myxobacteria |
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Definition
VEGETATIVE CELLS: non-flagellated rods that glide used in presence of food supply swarm w/coordinated motility FRUITING BODY: aggregation of cells present in nutrient depletion some cells become myxospores (resting) |
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Campylobacter Helicobacter pylori |
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microaerophiles motile spirals produces cholera-like toxin most common cause of enteritis |
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microaerophiles motile spirals cause acute & chronic gastritis-peptic ulcers produces Urease-neutralizes stomach acid |
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Low G+C positive bacteria |
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Definition
Mycoplasmas Clostridium Bacillus Staphylococcus Lactic acid bacteria |
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lack cell walls have sterols in plasma membrane to comp simplified metabolic pathways no TCA cycle Respiratory & urogenital tract infection |
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lack cell walls have sterols in plasma membrane to comp simplified metabolic pathways no TCA cycle Respiratory & urogenital tract infection |
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obligate anaerobes fermentative form endospores Botulism, tetanus, gas gangrene |
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aerobes or facultative aerobes form endospores Bt toxin >> insecticidal food poisoning, anthrax |
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On skin & mucus membranes of warm-blooded animals food poisoning, skin infections, MRSA |
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Depend on sugar fermentation >>lactic acid primarily used in food & dairy industry to make cheese & beer strep throat,pneumonia, cavities, UTIs |
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High G+C gram (+) bacteria |
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Definition
All are Actinobacteria/Actinomyces Corynebacterium Mycobacterium Propionibacterium Streptomyces Frankia Bifidobacterium |
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Definition
Use snapping division-palisade arrangement cause Diphtheria |
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slow growth* 2-40 days Mycolic acid in cell wall- Acid fast staining Tuberculosis, Leprosy |
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ferment sugars to propionic & acetic acid produce CO2> swiss cheese, acne, body odor |
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classic fungal characteristics: form aerial hyphae, chains of 3-50 nonmotile spores, perform mineralization 2/3 of antibiotics |
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Form clusters of nonmotile spores Can fix nitrogen mutualistic association w/non-legumes |
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Anaerobic ferments CHO* to acetic & lactic acids found in human mouth & GI tract makes up 90% of breast-fed infant gut flora |
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T4 bacteriophage controls the expression of its genes by regulating the activity of the hose _______________ in E.coli. |
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Definition
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The viral protein holin is used at what stage of replication of the T4 bacteriophage? |
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What viral enzyme catalyzes the synthesis of (viral) DNA using DNA as a template? |
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Definition
DNA-dependent DNA polymerase |
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A complex of proteins the virus uses/needs for genome packaging |
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Definition
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what degrades peptidoglycan in a cell wall infected by a virus during the entry and release stages? |
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Definition
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what type of genome do all archaeal viruses have? |
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Definition
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Integrates the viral genome into the host genome for lysogeny at the att site |
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Definition
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Protein that activates Integrase |
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Definition
CII protein (increase CII for cell to go through lysogeny) |
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Exicision of the viral DNA from host DNA/completion of the lytic cycle |
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Definition
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Definition
a host cell protein that causes lambda repressor to cleave itself when the cell is in danger or unhealthy. leads to induction |
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virus multiplies explosively, host cell lyses & dies |
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Definition
viral genome remains in neuron and can be reactivated at any point through environmental factors |
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NCLD (nucleo-cytoplasmic large DNA virus) |
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Definition
Eukaryotic virus with large virions & genomes relatively self-sufficient in metabolism* |
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Definition
a small virus that is a possible parasite of the NCLD virus (virophage: virus of a virus) |
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DNA that is complementary to viral mRNA |
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Definition
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a filamentous phage that attaches to the tip of sex pilus to infect (can only infect f+ bacteria) Made one at a time and secreted out of host |
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Definition
have a non-segmented genome that is essentially mRNA- no transcription necessary uses RNA-dependent RNA polymerase to replicate genome |
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the pcornaviruses such as poliovirus, are +ssRNA viruses, where their genome is used as a giant ________________ |
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Definition
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viroids are infectious agents made of ssRNA only that are asociated with diseases in __________ |
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Definition
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encode one or more gene products need helper virus to infect host cells |
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normal form of a protein found on cell membranes |
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abnormal form of a protein that affects all other surrounding proteins (a prion) by altering secondary structure causes neuron loss |
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