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shoreline community college Bio260 FINAL
Shoreline community college micro final
97
Microbiology
Undergraduate 1
06/10/2012

Additional Microbiology Flashcards

 


 

Cards

Term
Terms used to describe shape
Definition
Coccus pl cocci,
bacillus, pl; Bacilli,
Spirillum
Term
• Structural differences between Gram positive and Gram negative cell wall
Definition
Gram positive cell walls contain teichoic acid, have thick peptidoglycan layer, Lack outer membrane, lipopolysaccharide and porin proteins
Term
Why are electrons important in metabolism?
Definition
The movement of electrons helps cells make ATP
Term
Know difference between:
Chemotroph vs. Phototroph
Definition
Chemotrophs use chemical compounds for their energy source
Phototrophs use light as their energy source
Term
Why is nitrogen fixation important to all life on this planet?
Definition
Nitrogen fixation is important because cells use nitrogen to make amino acids and nucleic acids.
-Done by converting N2 to amino acids in a process of deamination.
Term
Obligate aerobe
Definition
require oxygen for growth
Term
Facultative Aerobe
Definition
Can live with or withut O2
Term
Obligate anaerobe
Definition
grows without the presence of O2
Term
Aerotolerant
Definition
can grow in the presence of oxygen but do not use it as its final electron acceptor.
Term
Microaerophile
Definition
Organisms that require small amounts of oxygen for growth but are inhibited by larger amounts.
Term
What is a mutation
Definition
• A mutation is a change in the nucleotide sequence of a cells DNA
Term
Spontaneous mutations include
Definition
Base substitution
removal or adding nucleotides
transposable elements
Term
Three results of base subtitutions
Definition
A silent mutation- Nucleotide change results in when new amino acid results in wild amino acid.
Missense mutation results when new codon encodes for a different amino acid.
Nonsense mutations. Occur when the changed codon is a stop codon.
Term
What are two structures that are found in some, but not all, viruses?
Definition
A matrix protein and an envelope.
Term
Anti-sense DNA can be used to make what?
Definition
Sense + DNA
Term
Describe the basic structural differences between bacteriophage and animal viruses.
Definition
Bacteriophage have tail region that are used to inject genetic material into host.
Animal virus enters host cell directly and can have additional outer coverings such as matrix protein and envelope.
Term
What’s the function of the tail structure in bacteriophage?
Definition
The tail structure attaches to the host cell and injects the viral nucleic acids into host.
Term
• What’s an operon?
Definition
An operon is a group of genes whose expression is controlled as a single unit.
Term
The genes of the lactose operon encode for proteins that do what?
Definition
Encodes for proteins required for degradation of lactose.
Term
cellular metabolism
Definition
breaking down and building up of molecules in order for cell to stay alive.
Term
Molecules needed for a cell to live
Definition
-Needs a molecule containing high energy electrons.
- molecule that has pull for electrons
-molecule containing carbons
N, S, P, Fe, Mg
Term
Catabolic reactions
Definition
break down molecules
Term
anabolic reactions
Definition
build molecues
Term
Organic molecules
Definition
Have H AND C (has to have both to be organic
Term
Inorganic
Definition
Lack H or C or both
Term
Chemoorganotroph
Definition
Use organic molecules as electron source.
Ex. Carbs, proteins, lipids
Term
chemolithotroph
Definition
Use Inorganic molecules as electron source.
H2 H2S NH3
Term
Phototroph
Definition
Use low energy inorganic and organic.
make high energy from sun
Term
Respirator
Definition
perform respiration

use inorganic molecules as electron acceptor.

O2, NO3, SO4
Term
2 types of respiration
Definition
aerobic respiration uses O2 as final electron acceptor

Anaerobic respirator uses NO3, SO4
Term
Fermentator
Definition
uses organic molecules as final electron acceptor

Ex: Pyruvate
Term
Autotroph
Definition
uses inorganic molecules as its source of carbon.
CO2

Auto (self)
troph(Feed)
Term
heterotroph
Definition
use organic molecules such as lipids, carbs, and proteins
Term
Cells that can metabolize organic molecules and O2
Definition
chemoorganotroph heterotroph fermentator aerobic respirator
Term
glucose is valuable because
Definition
it contains carbons
because it has high energy electrons.
Term
Lac Z encodes for
Definition
Beta-Galactosidase
A protein that serves as an enzyme that breaks up the glucose and galactose
Term
Lac A encodes for
Definition
Beta-galactoside transacetylase.

is the third protein that enables the bacteria to break down lactose.
Term
Lac Y encodes for
Definition
Galactoside permease
a pore protein that allows the E. coli to take the lactose in.
Term
(lac operon)
What’s the function of CAP-cAMP?
Definition
Helps RNA polymerase bind to the promoter region
Term
(lac operon)
What’s the function of the operator region?
Definition
A binding site for the repressor protein keeping the lac operon turned off
Term
What’s the function of the lac I gene
Definition
Contains information to tell the cell how to make the repressor protein.
Term
The presence of lactose causes E. coli to turn ( on or off ) its lactose operon and why?
Definition
On. The repressor protein changes shape when it comes in contact with lactose and falls off the operator region allowing the RNA polymerase to transcribe the lac operon. Which then produces the proteins that metabolize lactose.
Term
The presence of glucose causes E. coli to turn ( on or off ) its lactose operon.
Definition
Off.

In order to make ATP the AMP is used up leaving none available to bind to the cAMP. The Camp in turn changes shape rendering it unable to bind to the DNA molecules releasing the RNA polymerase from the promoter region shutting down the lac operon.
Term
How does the presence of lactose cause the lactose operon to get turned on?
Definition
The repressor protein changes shape when it comes in contact with lactose and falls off the operator region allowing the RNA polymerase to transcribe the lac operon. Which then produces the proteins that metabolize lactose.
Term
Bacteria Vs Virus
Definition
Bacteria are living, viruses are not.
Viruses lack ribosomes, ATP, ways of making ATP. No free floating AA or NA
Term
Basic structure of a virus
Definition
Nucleic acids wrapped in a protein coat.(capsid)
Some have a matrix protein and an envelope made up of phospholipids
Term
A virus that infects bacteria
Definition
bacteriophage
Term
Ways viruses can differ
Definition
Some contain Single stranded nucleic acid
some have double stranded NA's
Some have segmented NA, some have Non-segmented NA
Some have RNA
Some have DNA
Term
two types of infection caused by bacteriophage
Definition
Latent- don't produce complete viruses
productive- complete viruses are produced
Term
nuclease
Definition
encodes for nuclease enzyme that breaks down chromosomal DNA in the host cell.
Term
polymerase
Definition
reads NA and makes compliment of it.
Term
Early genes
Definition
Viral genes expressed immediately after entering the host cell. Part of productive infection
Term
two types of infection caused by Animal viruses
Definition
Latent
Productive
Term
HIV
Definition
has NA made of RNA
Term

(hiv)Viral RNA?

Process called?

Definition
is used to make complimentary DNA strand reverse transcription
Term
Animal viruses leave host cell via
Definition
Budding. Gains envelope by doing this.
lysing the cell
Term
Retrovirus
Definition
virus that does transcription in reverse.
Makes DNA from RNA
Term
two receptors needed for HIV gp120 to bind to and infect host cell
Definition
CD4 and a co-receptor (CxCR4 on helper T cells Or CCR5 found on macrophages, and dendritic cells)
Term
What allows fusion to happen?
Definition
GP40 embeds itself in the membrane of the host cell
Term
Types of cells HIV can infect
Definition
brain cells, MACs, Helper T-cells, Intestinal cells
Term
hallmark of advanced HIV infection
Definition
Low Helper t cells
Term
How does HIV cause Helper T cells die due to osmotic pressure?
Definition
Influx of NA and K ions which are co-factors required by enzymes for replication. This causes the cell to burst due to osmotic pressure.
Term
a. proteins responsible for detecting DNA damage do what?
b. If they are missing?
Definition
a. stop the cell from dividing
. Cell cant detect if its DNA is damaged, it will kill itself
Term
viral protein R (Vpr)
Definition
deactivates protein responsible for detecting damaged DNA
Term
apoptosis
Definition
cell mediated cell death
Term
Infected (helper T cell)CD4 molecule
Definition
loads antigen onto mhc 1 and presents to cytotoxic T cell. Cytotoxic T-cell recognizes antigen as foreign and kills the cell.
Term
GP120 causes decrease of helper t cells by?
Definition
binds to cd4 molecules keeping them from patrolling the blood for cells that need help
Term
free floating GP 120
Definition
binds to cd4 molecule of helper T cell causing antibodies to bind which then attracts Natural killer cells triggering ADCC (cell death)
Term
On average the time it takes for Hiv to overtake CD4 cells
Definition
is about 4 years
Term
-HIV infects
-causing
Definition
-CD4+ cells
-immediate depletion of CD4 cells
Term
Hiv infections decreases in the beginning because?
Definition
cytotoxic t cells and antibodies are able to respond and kill the infected cells.
Term
As number of CD4 cells go down...
causing???
Definition
-Cells are not able to release cytokines that activate cytotoxic T cells.
B-cells are not activated, so less antibodies are produced.
Term
four classes of drugs to treat HIV
Definition
1: Fusion inhibitors
2: reverse transcriptase inhibitor (stops DNA expression of HIV genome)
3: Inegrase inhibitor. Prevents HIV DNA from being intagrated into host DNA.
4: protease inhibitors. Prevent HIV from producing fully functioning proteins

3:
Term
Hiv is hard to target once it is inserts into DNA of host.
Definition
Because there are no drugs to target DNA without damaging host.
Term
attenuated vaccines
Definition
are weakened live viruses. HIV has high mutation rate and can mutate back to wild form.
Term
Inactivated vaccines
Definition
are dead viruses, Produces insufficient immune response. Doesn't work against all strains of HIV
Term
HIV GP120 epitopes
Definition
are hidden from antibodies by carbohydrate molecules. Keeping b-cells from binding and antibodies from being produced.
Term
Why inactivated HIV vaccines don't work against all strains.
Definition
Because HIV has a high mutation rate.
Term
Sub-unit vaccine
Definition
uses portions of the virus. Has same problems as inactivated virus
Term
DNA vaccines
Definition
-promising because you can use consistent HIV gene that does not differ between strains.

-stimulates both Ab and Tc responce
-Does not provie complete immunity
-not approved for use.
Term
b cells and t cells
Definition
are part of adaptive immune response
Term
Innate Immune Cells (macrophage and neutrophil)
Definition
ability to fight pathogens is encoded for by genes that don't change.

Built in immune system.
Term
Adaptive immune cells
Definition
can change (adapt) after coming into contact with pathogen.
Possess genes that are more prone to change.
Term
all blood cells come from....
Definition
come from hematopetic stem cells
Term
Common lymphoid progenitor
Definition
gives rise to cells in the adaptive immune system
Term
hematopetic stem cells are found in
Definition
the red bone marrow of the thymus, hips, tops of legs and shoulders.
Term
two types of lymphoid organs
Definition
-Primary lymphoid organs (bone marrow and thymus)

Secondary lymphoid organs: Lymph nodes, lymphatic, vessels, appendix, spleen and peyers patches. Secondary LOs are where pathogens are brought together with leukocytes.
Term
B-cells
Definition
bind to free floating epitopes.
Takes in Antigen, chops it up and presents it to helper t cell. Helper t cell confirms foreign antigen, releases cytokines and which activates B-cell
Term
T cells
Definition
only bind to epitopes if they are being presented by another cell.because TCR only recognizes epitope and MHC as a combo
Term
What happens when B-cells become activated?
Definition
B cell makes clones of itself. Some become Memory B cells and some become plasma cells. Plasma cells release antibodies specific to the antigen that originated the cloning of origional B-cell.
Term
Memory B cells have _______ cytokine receptors than original
Definition
More. this makes them more efficient should they encounter antigen again.
Term
When antibodies stick to a bacterial cell........
Definition
-can prevent bacterial cell from binding to our own cell (neutralization)

-can result in compliment proteins to become activated

- NK cell can binds to antibodies bound to antigens and causes ADCC

-agglutination
Term
ADCC is?
Definition
Antibody dependent cell mediated cytotoxicity
Term
helper t cells activate..
Definition
B cells, macrophages, NK cells and cytotoxic T cells.
Term
CD4 Molecules are found on..
CD8 Molecules are found on..
Definition
a helper T cells
B Cytotoxic T cell
Term
Cytotoxic T cells release?
causing the cell to die how? why?
Definition
perforins and proteases.
By apoptosis.
Lysing the cell would release the virus with in the cell. apoptosis makes the cell shrivel up and be eaten by a macrophage.
Term
Some viruses avoid being killed by cytotoxic T-calls how?
Definition
by down regulating the expression of the MHC 1 molecule in the host.
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