Term
| For the following cards, the SERUM ENZYME will be provided and the "Definition" side would have the general Diagnostic use of that enzyme |
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Definition
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Term
| Aspartate aminotransferase (AST or SGOT) |
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Definition
Myocardial Infarction (but also found in Liver)
AST did not have a * next to it, so we don't have to "know" it for the Unit 5 exam. All that follow are *'ed |
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Term
| Alanine Aminotransferase (ALT or SGPT) |
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Definition
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Term
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Definition
Acute Pancreatitis
Amylase --> think digestion enzyme = pancreas |
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Term
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Definition
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Term
| Lactate Dehydrogenase isozyme 5 |
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Definition
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Term
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Definition
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Term
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Definition
| Metastatic Carcinoma (prostate) |
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Term
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Definition
| Various BONE disorders, obstructive liver diseases |
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Term
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Definition
| Different forms of an enzyme that carry out the same general reaction. |
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Term
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Definition
TISSUE SPECIFIC:
One isozyme may be SPECIFICALLY expressed for one TISSUE/ORGAN, while another isozyme for another
Isozymes may have a SPECIFIC pattern of EXPRESSION during DEVELOPMENT |
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Term
| LDH and CK are examples of... |
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Definition
| ...ISOZYMES. LDH has 5 forms of isozymes and CK has 3. LDH and CK are not isozymes of each other though! |
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Term
| Describe LDH (Lactate Dehydrogenase) |
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Definition
4 subunits. 2 types of subunits --> alpha/Beta
LDH-1 - 4a (heart)
LDH-2 - 3a,1B
LDH-3 - 2a,2B
LDH 4 - 1a,3B
LDH 5 - 4B --> DIAGNOSTIC for LIVER DISEASE! |
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Term
| LDH 5 (4B) is diagnostic for... |
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Definition
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Term
| Describe Creatine Kinase (CK) |
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Definition
2 Subunits, 2 types of subunits (M and B)
BB = CK1 --> Brain, Lung, Thyroid
MB = CK2 --> ONLY in Cardiac! --> thus VERY Diagnostic
MM = CK3 --> skeletal and cardiac muscle |
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Term
| One way to distinguish LDH isozymes is via... |
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Definition
| ..ELECTROPHORESIS b/c they have different overall charges! |
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Term
| How can you use LDH and CK activities if a patient had an MI (CARDIAC MUSCLE)? |
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Definition
Look for CK-2 (MB) and LDH-1 (4 alpha's) in the plasma.
In particular, both enzyme activities appear TRANSIENTLY after attack. |
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Term
| How about for muscular dystrophy (Skeletal Muscle)? |
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Definition
| Look for CK-3 (BB). In particular, a LONG-TERM rise of CK-3 activity in the plasma. |
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