Term
| What are the steps of the KOH wet mount procedure? |
|
Definition
1. Place 10% KOH on slide
2. Emulsify specimen in KOH
3. Apply Cover Slip
4. Gently heat
5. Examine with 40x objective |
|
|
Term
|
Definition
Blastomyces dermatitidis
Coccidioides immitis
Histoplasma capsolatum
Paracoccidioides
Sporothrix schenkii |
|
|
Term
| What is the appearance of the dimorphic fungi at 37 degrees Celsius? |
|
Definition
|
|
Term
| What is the appearance of the dimorphic fungi at RT? |
|
Definition
|
|
Term
With respect to Malassezia furfur:
What special media should be used to culture for this organism? |
|
Definition
Media supplemented with lipids;
Olive oil can also be added to culture media |
|
|
Term
With respect to Malassezia furfur:
What type of patient is at risk for systemic disease? |
|
Definition
| Patients on lipid therapy |
|
|
Term
With respect to Malassezia furfur:
Name 2 diseases this organism causes |
|
Definition
Tinea versicolor
Bacteremia
Infected IV catheters |
|
|
Term
| What is the recommended sputum collection procedure? |
|
Definition
| Three 1st morning specimens |
|
|
Term
| What is the usual clinical significance of a Penicillium isolate? |
|
Definition
| Frequently a contaminant although it can cause disease in some individuals |
|
|
Term
Put the following in order with respect to their ability to detect cryptococcal meningitis:
India Ink Prep
Culture
AGN Test |
|
Definition
| Culture > AGN Test > India Ink Prep |
|
|
Term
| What effect does cycloheximide have on Cryptococcus? |
|
Definition
|
|
Term
With respect to Nocardia:
What is its gram-stain reaction? |
|
Definition
| GP Branching Rods that may bead |
|
|
Term
With respect to Nocardia:
What other organism may also have this same appearance? |
|
Definition
|
|
Term
With respect to Nocardia:
What stain might rapidly distinguish Nocardia from Actinomyces? |
|
Definition
|
|
Term
| What disease does Pneumocystis jirovec typically cause? |
|
Definition
|
|
Term
| What stains can be used to visualize Histoplasma capsulatum and Pneumocystis jirovec in specimen smears? |
|
Definition
| Giemsa & Methenamine silver stain |
|
|
Term
| What stains are NOT helpful in visualizing Histoplasma capsulatum and Pneumocystis jirovec in specimen smears? |
|
Definition
|
|
Term
For each of the following dermatophytes, describe (in general terms) the characteristic macroconidia:
epidermophyton |
|
Definition
|
|
Term
For each of the following dermatophytes, describe (in general terms) the characteristic macroconidia:
Trichophyton |
|
Definition
|
|
Term
For each of the following dermatophytes, describe (in general terms) the characteristic macroconidia:
Microsporum |
|
Definition
|
|
Term
Given the MO, what is the characteristic morphology of its yeast phase:
Blastomyces dermatitidis |
|
Definition
Broad-Based Budding
Figure 8
Double Walled |
|
|
Term
Given the MO, what is the characteristic morphology of its yeast phase:
Histoplasma capsulatum |
|
Definition
Small narrow-based budding
Oval to Round |
|
|
Term
Given the MO, what is the characteristic morphology of its yeast phase:
Coccidioides Immitis - Known as "Valley Fever" |
|
Definition
| Spherules with endospores |
|
|
Term
Given the MO, what is the characteristic morphology of its yeast phase:
Sporothrix schenckii |
|
Definition
|
|
Term
Given the MO, what is the characteristic morphology of its mold phase:
Histoplasma capsulatum |
|
Definition
|
|
Term
Given the MO, what is the characteristic morphology of its mold phase:
Coccidioides Immitis - Known as "Valley Fever" |
|
Definition
| Alternating Barrel-shaped arthrospores |
|
|
Term
Given the MO, what is the characteristic morphology of its mold phase:
Sporothrix schenckii |
|
Definition
| Conidia in a "flowerette" arrangement |
|
|
Term
Given the MO, what is the characteristic morphology of it on cornmeal agar:
Candida albicans |
|
Definition
|
|
Term
Given the MO, what is the characteristic morphology of it upon incubation in serum for 2 hours:
Candida albicans |
|
Definition
|
|
Term
Given the MO, what is its characteristic morphology:
Penicillium species |
|
Definition
|
|
Term
Given the MO, what is its characteristic morphology:
Aspergillus species |
|
Definition
|
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