Term
| Is Pseudomonas aeruginosa Lactose + or =? |
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Definition
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Term
True or False:
Acinetobacter may resemble enterics |
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Definition
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Term
| What pigment is characteristic of Flavobacteria? |
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Definition
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Term
| What pigment is characteristic of Chromobacterium? |
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Definition
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Term
| Is Stenotrophomonas maltophilia Trimethoprim-sulfamethoxazole Susceptible or Resistant? |
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Definition
| Trimethoprim-sulfamethoxazole Susceptible |
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Term
| What colony characteristic of pseudomonas aeruginosa is often observed in isolates recovered from cystic fibrosis patients? |
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Definition
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Term
| What is the difference in biochemical test reactions between Klebsiella pneumoniae and K. oxytoca? |
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Definition
K. oxytoca Indole +
K. pneumoniae Indole = |
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Term
| What test should be performed to definitively identify Shigella? |
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Definition
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Term
| What should be done if the serogrouping for Shigella does not work the first time? |
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Definition
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Term
| What test should be performed to definitively identify Salmonella? |
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Definition
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Term
| What should be done if the serogrouping for Salmonella does not work the first time? |
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Definition
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Term
| Which type of Salmonella is associated with "Rose spots" (red spots) on the abdomen? |
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Definition
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Term
What is the purpose of the following media:
Anaerobic PEA |
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Definition
Inhibits facultative GNRs
Supports growth of Facultative GP MOs
Anaerobes (GP & GN) |
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Term
What is the purpose of the following media:
Bacteroides Bile-esculin |
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Definition
| Selective & Differential for Bacteroides fragilis group |
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Term
What is the purpose of the following media:
Kanamycin-vancomycin laked blood agar (KVLB) |
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Definition
Selects for Bacteroides & Prevotella
Enhances pigment production by certain Prevotella species |
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Term
Given the key identification characteristics of a known anaerobe MO, name the MO:
Reverse CAMP +
Lecithinase + |
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Definition
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Term
Given the key identification characteristics of a known anaerobe MO, name the MO:
Bile Resistant
Kanamycin Resistant
Vancomycin Resistant
Colistin Resistant |
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Definition
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Term
Given the key identification characteristics of a known anaerobe MO, name the MO:
Catalase +
Indole + |
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Definition
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Term
Given the key identification characteristics of a known anaerobe MO, name the MO:
SPS Disk Susceptible |
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Definition
| Peptostreptococcus anaerobius |
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Term
Given the biochemical tests, name the GN MO:
Phenylalanine =
VP =
Indole +
H2S =
Citrate =
Lysine +
Lactose +
Motility +
TSI - A/A (with gas) |
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Definition
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Term
Given the biochemical tests, name the GN MO:
PA =
VP =
Citrate =
H2S =
Lysine =
Lactose =
Motility =
TSI - K/A |
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Definition
|
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Term
Given the biochemical tests, name the GN MO:
PA =
VP =
H2S +
Lysine +
Citrate +
Lactose =
ONPG =
TSI - K/A with H2S |
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Definition
|
|
Term
Given the biochemical tests, name the GN MO:
PA =
VP +
Lactose +
Ornithine =
Motility =
TSI - A/A with H2S |
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Definition
|
|
Term
Given the biochemical tests, name the GN MO:
PA =
VP +
Lactose +
Motility +
Ornithine +
DNAse =
TSI - A/A |
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Definition
|
|
Term
Given the biochemical tests, name the GN MO:
PA =
VP +
DNAse +
Lactose =
Motility +
Ornithine +
TSI - A/A |
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Definition
|
|
Term
Given the biochemical tests, name the GN MO:
PA +
H2S +
VP =
Ornithine =
Indole +
TSI - A/A with H2S |
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Definition
|
|
Term
Given the biochemical tests, name the GN MO:
PA +
H2S +
Indole =
VP =
Ornithine =
TSI - K/A with H2S |
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Definition
|
|
Term
Given the biochemical tests, name the GN MO:
Oxidase +
Nonfermenter
Glucose Oxidizer
Growth at 42 degrees Celsius
TSI - K/K or K/No Change |
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Definition
|
|
Term
| Is Stenotrophomonas maltophilia Oxidase + or =? |
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Definition
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Term
| Is Acinetobacter Oxidase + or =? |
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Definition
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Term
Which type of Salmonella shows the following:
small amount of H2S
"Mustache"
TSI or K/A |
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Definition
|
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Term
| Is Acinetobacter Nitrate + or =? |
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Definition
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Term
What is the Principle/Procedure for the following test:
Bile-esculin |
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Definition
40% Bile inhibits many MOs
Esculin --> Esculetin + Glucose
Esculetin + Ferricions --> Black |
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|
Term
What does a positive result look like for the following test:
Bile-esculin |
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Definition
|
|
Term
What does a negative result look like for the following test:
Bile-esculin |
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Definition
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Term
What is the Principle/Procedure for the following test:
Bile-solubility |
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Definition
Pneumococcal autolysis is accelerated by bile
Plate methd - add bile to colonies
Tube method - Add bile to suspension of MOs |
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|
Term
What does a positive result look like for the following test:
Bile-solubility |
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Definition
|
|
Term
What does a negative result look like for the following test:
Bile-solubility |
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Definition
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Term
What is the Principle/Procedure for the following test:
CAMP |
|
Definition
GBS make CAMP factor
S. aureus makes Beta-lysin
Enhance zone of hemolysis appears when CAMP factor meets Beta-lysin
Test MO steaked perpendicular to a S. aureus streak |
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Term
What does a positive result look like for the following test:
CAMP |
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Definition
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|
Term
What does a negative result look like for the following test:
CAMP |
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Definition
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Term
What is the Principle/Procedure for the following test:
Carbohydrate-rapid utilization |
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Definition
Acid formed when carbohydrate used.
Phenol red detects pH change
Used to identify Neisseria |
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|
Term
What does a positive result look like for the following test:
Carbohydrate-rapid utilization |
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Definition
|
|
Term
What does a negative result look like for the following test:
Carbohydrate-rapid utilization |
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Definition
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Term
What is the Principle/Procedure for the following test:
Catalase |
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Definition
H2O2 -(catalase)-> H2O + O2
Place MO on microscope slide and add drop of H2O2 |
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Term
What does a positive result look like for the following test:
Catalase |
|
Definition
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|
Term
What does a negative result look like for the following test:
Catalase |
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Definition
|
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Term
What is the Principle/Procedure for the following test:
Citrate utilization (Simmons citrate agar) |
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Definition
Determines if MO can use citrate as sole source of carbon
Medium becomes alkaline if isolate grows
pH indicator - Bromthymol Blue |
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|
Term
What does a positive result look like for the following test:
Citrate utilization (Simmons citrate agar) |
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Definition
|
|
Term
What does a negative result look like for the following test:
Citrate utilization (Simmons citrate agar) |
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Definition
|
|
Term
| Which organism is bile soluble? |
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Definition
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Term
| Which organism is CAMP Positive? |
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Definition
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Term
What is the Principle/Procedure for the following test:
Coagulase-Slide |
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Definition
Fibrinogen --(cell bound coagulase)-> Fibrin strands
Prepare saline suspension of test MO on microscope slide.
Mix in drop of plasma |
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|
Term
What does a positive result look like for the following test:
Coagulase-Slide |
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Definition
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|
Term
What does a negative result look like for the following test:
Coagulase-Slide |
|
Definition
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Term
What is the Principle/Procedure for the following test:
Coagulase-Tube |
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Definition
Fibrinogen -(coagulase)-> Fibrin clot
Add test MO to tube of Plasma & Incubate for 4 hours @ 35 degrees Celsius
Hold Negative tubes overnight @ RT |
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|
Term
What does a positive result look like for the following test:
Coagulase-Tube |
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Definition
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|
Term
What does a negative result look like for the following test:
Coagulase-Tube |
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Definition
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Term
What is the Principle/Procedure for the following test:
Hippurate hydrolysis |
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Definition
Detect either benzoate or glycine
Hippurate -(hippuricase)-> Sodium benzoate + Glycine
Benzoate + Ferric Chloride -> Precipitate
Glycine+Ninhydrin --> Blue |
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|
Term
What does a positive result look like for the following test:
Hippurate hydrolysis |
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Definition
Benzoate - Precipitate
Glycine - Blue |
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Term
What does a negative result look like for the following test:
Hippurate hydrolysis |
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Definition
Benzoate - No precipitate or clearing of precipitate
Glycine - No Color |
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Term
What is the Principle/Procedure for the following test:
Hydrogen Sulfide (H2S) production |
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Definition
Suflur containing AA or Inorganic sulfur compounds --(some MOs)--> H2S
H2S + Iron or Lead --> Black |
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Term
What does a positive result look like for the following test:
Hydrogen Sulfide (H2S) production |
|
Definition
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|
Term
What does a negative result look like for the following test:
Hydrogen Sulfide (H2S) production |
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Definition
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|
Term
What is the Principle/Procedure for the following test:
Indole (Spot & Tube) |
|
Definition
Tryptophan -(tryptophanase)-> Indole
Indole + Reagent -> color
Spot: p-dimethylaminocinnamaldehyde
Tube: Kovac's or Ehrlich's Reagent which has p-dimethylaminobenzaldehyde |
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|
Term
What does a positive result look like for the following test:
Indole (Spot & Tube) |
|
Definition
Spot: Blue-Green
Tube: Red |
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|
Term
What does a negative result look like for the following test:
Indole (Spot & Tube) |
|
Definition
Spot: No Color Change
Tube: No Color Change |
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Term
| What else is Coagulase-slide testing known as? |
|
Definition
| Bound Coagulase or Clumping Factor |
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|
Term
True or False:
Systems & Media vary in their ability to detect H2S production |
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Definition
|
|
Term
What is the Principle/Procedure for the following test:
Nitrate Reduction |
|
Definition
Nitrate --> Nitrite --> N2 Gas
Nitrite detected by adding sulfanilic acid & Dimetthyl-alpha-napthylamine |
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|
Term
What does a positive result look like for the following test:
Nitrate Reduction |
|
Definition
Red after addition of reagents
OR
Colorless after reagents & Zinc added |
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|
Term
What does a negative result look like for the following test:
Nitrate Reduction |
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Definition
| Red after Reagents & Zinc Added |
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|
Term
What is the Principle/Procedure for the following test:
ONPG |
|
Definition
| Orthonitrophenylglactopyranoside (colorless) -(Beta-galactosidase)-> Orthonitrophenol + galactose |
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|
Term
What does a positive result look like for the following test:
ONPG |
|
Definition
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|
Term
What does a negative result look like for the following test:
ONPG |
|
Definition
|
|
Term
What is the Principle/Procedure for the following test:
Oxidase |
|
Definition
| Tetra OR di-methyl-p-phenylenediamine dihydrochloride (oxidase reagent) --(cytochrome oxidase)--> indophenol |
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|
Term
What does a positive result look like for the following test:
Oxidase |
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Definition
| Dark purple or Blue-Black |
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|
Term
What does a negative result look like for the following test:
Oxidase |
|
Definition
|
|
Term
What is the Principle/Procedure for the following test:
Phenylalanine deaminase |
|
Definition
Phenylalanine deaminase --(deaminase)--> phenylpyruvic acid
Phenylpyruvic acid + Ferric Chloride --> Green |
|
|
Term
What does a positive result look like for the following test:
Phenylalanine deaminase |
|
Definition
|
|
Term
What does a negative result look like for the following test:
Phenylalanine deaminase |
|
Definition
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|
Term
What is the Principle/Procedure for the following test:
PYR |
|
Definition
Pyrrolidonyl-Beta-naphthylamide--(pyroglutamyl aminopeptidase)--> Free Beta-naphthylamine
N,N dimethylaminocinnamaldegyde added to detect Beta-naphthylamine |
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|
Term
What does a positive result look like for the following test:
PYR |
|
Definition
|
|
Term
What does a negative result look like for the following test:
PYR |
|
Definition
|
|
Term
What is the Principle/Procedure for the following test:
Salt Tolerance (6.5%) |
|
Definition
6.5% salt inhibits many MOs
Inoculate brother with test MO |
|
|
Term
What does a positive result look like for the following test:
Salt Tolerance (6.5%) |
|
Definition
|
|
Term
What does a negative result look like for the following test:
Salt Tolerance (6.5%) |
|
Definition
| No Turbidity OR pH Change |
|
|
Term
What is the Principle/Procedure for the following test:
Satellite Test |
|
Definition
Satelliting MOs use excess nutrients produced by S. aureus
MO inoculated onto BA. S. aureus then streaked through inoculated area. |
|
|
Term
What does a positive result look like for the following test:
Satellite Test |
|
Definition
| Tiny clonies growing near the staph streak |
|
|
Term
What does a negative result look like for the following test:
Satellite Test |
|
Definition
| No tiny colonies growing near the staph streak |
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|
Term
What is the Principle/Procedure for the following test:
Staph Latex Test |
|
Definition
Latex beads coated with antibody to protein A.
Mix test MO with latex reagent |
|
|
Term
What does a positive result look like for the following test:
Staph Latex Test |
|
Definition
|
|
Term
What does a negative result look like for the following test:
Staph Latex Test |
|
Definition
|
|
Term
What is the Principle/Procedure for the following test:
Urease |
|
Definition
Urea--(urease)--> Ammonia
Medium becomes alkaline as ammonia is produced
pH Indicator: Phenol Red |
|
|
Term
What does a positive result look like for the following test:
Urease |
|
Definition
|
|
Term
What does a negative result look like for the following test:
Urease |
|
Definition
|
|
Term
What is the Principle/Procedure for the following test:
Voges-Proskauer (VP) |
|
Definition
Glucose --> Acetoin (acetylmethylcarbinol)
Acetoin + Alpha-naphthol + 40% KOH --> Red |
|
|
Term
What does a positive result look like for the following test:
Voges-Proskauer (VP) |
|
Definition
|
|
Term
What does a negative result look like for the following test:
Voges-Proskauer (VP) |
|
Definition
|
|
Term
What is the Principle/Procedure for the following test:
X,V, & XV Factor Disks |
|
Definition
Test MO inoculated onto TSA or MHA which lack X factor (hemin) and V Factor (NAD)
Paper Disk with X, V, or XV p-laced onto inoculated area |
|
|
Term
What does a positive result look like for the following test:
X,V, & XV Factor Disks |
|
Definition
|
|
Term
What does a negative result look like for the following test:
X,V, & XV Factor Disks |
|
Definition
|
|
Term
| Which nutrient is required by satelliting streptococci? |
|
Definition
|
|
Term
| What nutrient is supplied for Haemophilus? |
|
Definition
| NAD must be supplied for V requiring Haemophilus |
|
|
Term
What does a positive result look like for the following:
Spot Indole |
|
Definition
|
|
Term
What does a negative result look like for the following:
Spot Indole |
|
Definition
|
|
Term
What does a positive result look like for the following:
Tube Indole |
|
Definition
|
|
Term
What does a negative result look like for the following:
Tube Indole |
|
Definition
|
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