Term
| What procedure(s) should be followed in medicolegal cases when an isolate appears to be Neisseria gonorrhoeae? |
|
Definition
| Use 2 different methodologies to ID |
|
|
Term
| What is the purpose of GN broth? |
|
Definition
| Enrichment for Salmonella and Shigella |
|
|
Term
What is the significance of the following urine colony counts:
One organism at 10^5 CFU/ml? |
|
Definition
Contaminant/Contaminated specimen
-probably causing UTI |
|
|
Term
What is the significance of the following urine colony counts:
Three MOs in equal numbers? |
|
Definition
|
|
Term
With respect to clue cells:
What are they? |
|
Definition
| Vaginal epis coated with bacteria |
|
|
Term
With respect to clue cells:
What disease are they associated with? |
|
Definition
|
|
Term
With respect to clue cells:
What organism are they associated with? |
|
Definition
|
|
Term
| How can gram-stained smears be used in the interpretation of culture results? |
|
Definition
Presence of epis indicate contamination. specimen w/WBCs and no epis is of higher quality.
MOs observed in smears prepped from high quality specimens are probably significant. |
|
|
Term
| Name 6 organisms that are part of the normal flora of humans |
|
Definition
Strep
Neisseria
Staph
Haemophilus
Many enterics
Corynebacterium
Enterococci
Anaerobic |
|
|
Term
How do humans typically become infected by the following organisms?
Brucella |
|
Definition
|
|
Term
How do humans typically become infected by the following organisms?
Pasteurella multocida |
|
Definition
|
|
Term
How do humans typically become infected by the following organisms?
Aeromonas |
|
Definition
|
|
Term
How do humans typically become infected by the following organisms?
Legionella |
|
Definition
|
|
Term
How do humans typically become infected by the following organisms?
Chlamydia trachomatis |
|
Definition
| Trachoma (direct contact), genital, & lymphogranuloma venereum (sexual contact) & inclusion conjunctivitis (female genital tract) |
|
|
Term
What is the disease often associated with the following organism:
Legionella |
|
Definition
Pontaic Fever
Legionnaire's Disease |
|
|
Term
What is the desired specimen for the following organism:
Chlamydia trachomatis |
|
Definition
|
|
Term
| Laboratories must perform QC on certain media even though the manufacturer has documented that the media meets NCCLS standards. Name 3 of these media. |
|
Definition
Gonococcal Selective Media (MTM, ML)
Campylobacter Selective Media
Chocolate Agar |
|
|
Term
What is the typical colony morphology of E. coli on the following media:
EMB |
|
Definition
|
|
Term
What is the typical colony morphology of E. coli on the following media:
MAC |
|
Definition
| Red/Pink with precipitated bile |
|
|
Term
What is the typical colony morphology of E. coli on the following media:
SS |
|
Definition
|
|
Term
What is the typical colony morphology of E. coli on the following media:
Hektoen |
|
Definition
|
|
Term
What is the typical colony morphology of E. coli on the following media:
Xylose-Lysine-Deoxycholate |
|
Definition
|
|
Term
What is the typical colony morphology of Klebsiella & Enterobacter on the following media:
EMB |
|
Definition
|
|
Term
What is the typical colony morphology of Klebsiella & Enterobacter on the following media:
MAC |
|
Definition
| Red/Pink with precipitated bile |
|
|
Term
What is the typical colony morphology of Klebsiella & Enterobacter on the following media:
SS |
|
Definition
|
|
Term
What is the typical colony morphology of Klebsiella & Enterobacter on the following media:
Hektoen |
|
Definition
|
|
Term
What is the typical colony morphology of Klebsiella & Enterobacter on the following media:
XLD |
|
Definition
|
|
Term
What is the typical colony morphology of Shigella on the following media:
EMB |
|
Definition
|
|
Term
What is the typical colony morphology of Shigella on the following media:
MAC |
|
Definition
|
|
Term
What is the typical colony morphology of Shigella on the following media:
SS |
|
Definition
|
|
Term
What is the typical colony morphology of Shigella on the following media:
Hektoen |
|
Definition
|
|
Term
What is the typical colony morphology of Shigella on the following media:
XLD |
|
Definition
|
|
Term
What is the typical colony morphology of Salmonella on the following media:
EMB |
|
Definition
|
|
Term
What is the typical colony morphology of Salmonella on the following media:
MAC |
|
Definition
|
|
Term
What is the typical colony morphology of Salmonella on the following media:
SS |
|
Definition
| Colorless with black centers |
|
|
Term
What is the typical colony morphology of Salmonella on the following media:
Hektoen |
|
Definition
| Blue-green to Blue with black centers |
|
|
Term
What is the typical colony morphology of Salmonella on the following media:
XLD |
|
Definition
|
|
Term
| What is the typical colony morphology of nonfermenters on MacConkey agar? |
|
Definition
Lactose Negative if they grow
(not all are capable of growing on MAC) |
|
|
Term
True or False:
The following MOs all have GPC in clusters on their gram stain:
Staphylococcus aureus
Staph. saprophyticus
Other coag-negative staph |
|
Definition
|
|
Term
True or False:
The following MOs all have GPC in chains on their gram stain:
GAS
GBS
Enterococci
GDS
Viridans streptococci |
|
Definition
|
|
Term
What is the gram-stain results typical of the following:
Micrococcus |
|
Definition
| GPC, sometimes in tetrads |
|
|
Term
What is the gram-stain results typical of the following:
Pneumococci |
|
Definition
| Lancet-shaped GP Diplococci |
|
|
Term
What is the gram-stain results typical of the following:
Bacillus species |
|
Definition
|
|
Term
What is the gram-stain results typical of the following:
Listeria |
|
Definition
|
|
Term
True or False:
The following MOs all have kidney-bean shaped GNDC:
Moraxella catarrhalis
N. gonorrhoeae
N. meningitidis |
|
Definition
|
|
Term
What is the gram-stain results typical of the following:
Lactobacillus |
|
Definition
|
|
Term
True or False:
The following MOs all have small pleomorphic GNR:
Haemophilus influenzae
Haemophilus parainfluenzae |
|
Definition
|
|
Term
True or False:
The following MOs all have faintly staining "gull wings" GNR:
Helicobacter pylori
Campylobacter jejuni |
|
Definition
|
|
Term
What is the gram-stain results typical of the following:
Aeromonas |
|
Definition
|
|
Term
What is the gram-stain results typical of the following:
Acinetobacter |
|
Definition
| GNR, May be coccobacillary |
|
|
Term
True or False:
The following MOs all have GNR:
Flavobacteria
Chromobacterium
Eikenella
Stenotrophomonas maltophilia
Pseudomonas aeruginosa |
|
Definition
|
|
Term
What is the hemolysis of the following:
Staph aureus |
|
Definition
|
|
Term
What is the hemolysis of the following:
GAS |
|
Definition
|
|
Term
What is the hemolysis of the following:
GBS |
|
Definition
|
|
Term
What is the hemolysis of the following:
Enterococci |
|
Definition
|
|
Term
What is the hemolysis of the following:
GDS |
|
Definition
|
|
Term
What is the hemolysis of the following:
Pneumococci |
|
Definition
Alpha
Mucoid / Umbilicated (indented) |
|
|
Term
What is the hemolysis of the following:
Viridans streptococci |
|
Definition
|
|
Term
What is the hemolysis of the following:
Bacillus species |
|
Definition
|
|
Term
What is the hemolysis of the following:
Listeria |
|
Definition
|
|
Term
What is the hemolysis of the following:
Corynebacterium species (diptheroids) |
|
Definition
|
|
Term
What is the hemolysis of the following:
Lactobacillus |
|
Definition
|
|
Term
| What is a colony characteristic of N. gonorrhoeae? |
|
Definition
| May produce small and large colonies |
|
|
Term
| What is the colony characteristic of Staph aureus on Mannitol salt agar? |
|
Definition
|
|
Term
| What is the colony characteristic of Other coagulase-negative staph on Mannitol salt agar? |
|
Definition
|
|
Term
| What is the colony characteristic of Micrococcus? |
|
Definition
|
|
Term
| What is the colony characteristic of Haemophilus influenzae? |
|
Definition
|
|
Term
| What is the colony characteristic of Campylobacter jejuni? |
|
Definition
| Moist spreading colonies that tend to form streaklines |
|
|
Term
Given the key biochemical test, Name the MO:
Catalase +
Coagulase +
Latex Agglutination + |
|
Definition
|
|
Term
Given the key biochemical test, Name the MO:
Catalase +
Coagulase =
Novobiocin Resistant |
|
Definition
| Staphylococcus saprophyticus |
|
|
Term
Given the key biochemical test, Name the MO:
Catalase +
Coagulase =
Novobiocin Susceptible |
|
Definition
Other Coagulase-Negative Staph
(e.g., S. epidermidis) |
|
|
Term
Given the key biochemical test, Name the MO:
Catalse =
Bacitracin Disk Susceptible
PYR + |
|
Definition
|
|
Term
Given the key biochemical test, Name the MO:
Catalase =
Hippurate +
CAMP + |
|
Definition
|
|
Term
Given the key biochemical test, Name the MO:
Catalase =
Bile esculin +
6.5% NaCl +
PYR + |
|
Definition
|
|
Term
Given the key biochemical test, Name the MO:
Catalase =
Bile esculin +
6.5% NaCl = |
|
Definition
|
|
Term
Given the key biochemical test, Name the MO:
Catalase =
Optocin Disk Susceptible
Bile soluble |
|
Definition
|
|
Term
Given the key biochemical test, Name the MO:
Catalase =
Bile esculin =
6.5% NaCl =
Optochin Disk Resistant
NOT Bile Soluble |
|
Definition
|
|
Term
Given the key biochemical test, Name the MO:
Grows aerobically
Forms spores |
|
Definition
|
|
Term
Given the key biochemical test, Name the MO:
Catalase +
Motility + @ RT
Motility = @ 37 degrees Celsius
Bile esculin +
"Umbrella" |
|
Definition
|
|
Term
Given the key biochemical test, Name the MO:
Gamma Hemolysis
Catalase +
"Chinese letters" |
|
Definition
|
|
Term
Given the key biochemical test, Name the MO:
GPR
Catalase =
Gamma & Alpha Hemolysis |
|
Definition
|
|
Term
| What can you do in determining GAS to enhance hemolysis? |
|
Definition
|
|
Term
| What disease is often associated with Staph saprophyticus? |
|
Definition
|
|
Term
| What important disease is often associated with GBS? |
|
Definition
|
|
Term
| What is the Quellung reaction? |
|
Definition
| Pneumococcal ABY is mixed on a slide with a possible pneumococcal isolate. If the ABY matches the capsular AGN, an optical illusion occurs when the mixture is examined microscopically. The capusule appears to swell. |
|
|
Term
| What are the culture conditions for growing N. gonorrhoeae? |
|
Definition
|
|
Term
Given the key biochemical test, Name the MO:
Oxidase +
Glucose +
Maltose =
Lactose =
Sucrose = |
|
Definition
|
|
Term
Given the key biochemical test, Name the MO:
Oxidase +
Glucose +
Maltose +
Lactose =
Sucrose = |
|
Definition
|
|
Term
Given the key biochemical test, Name the MO:
Oxidase +
Glucose =
Maltose =
Lactose =
Sucrose =
DNAse +
Nitrate + |
|
Definition
|
|
Term
Given the key biochemical test, Name the MO:
Requires X & V factor
Porphyrin Test = |
|
Definition
|
|
Term
Given the key biochemical test, Name the MO:
Requires V Factor
Porphyrin Test + |
|
Definition
| Haemophilus parainfluenzae |
|
|
Term
Given the key biochemical test, Name the MO:
GNR
Oxidase + |
|
Definition
|
|
Term
Given the key biochemical test, Name the MO:
Oxidase +
Catalase +
Darting motility |
|
Definition
|
|
Term
Given the key biochemical test, Name the MO:
Oxidase +
Urease +
Causes gastric and duodenal ulcers |
|
Definition
|
|
Term
| What are the culture conditions for Haemophilus influenzae? |
|
Definition
Choc in CO2
Satellite around staph |
|
|
Term
| What are the culture conditions for Campylobacter jejuni? |
|
Definition
Grows on CAMPY-BA
Microaerobic @ 42 degrees Celsius |
|
|
