Term
Adenosine triphosphate (ATP) |
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Definition
Has high potential energy and allows cells to do work. As cells process sugar, the energy that is released is used to transfer a phosphate group to adenosine diphosphate (ADP) |
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Definition
reactions that release energy |
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Definition
Reactions that require energy |
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Definition
The addition of a phosphate group to a substrate; phosphorylation of proteins is exergonic because the electrons in ADP and the phosphate group have much less potential energy than they did in ATP. |
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Term
Reduction-oxidation reactions |
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Definition
a class of chemical reactions that involve the loss or gain of one or more electrons; they drive the formation of ATP. |
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Term
Nicotinamide adenine dinuleotide (NAD+) |
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Definition
Reduced from NADH which readily donates electrons to other molecules and is said to have "reducing power." |
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Term
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Definition
One molecuel fo glucose is broken into two molecules of the three-carbon compound pyruvate.
2 ATP molecules are produced from ADP and 1 molecule of NAD+ is reduced to form NADH. |
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Term
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Definition
Pyruvate is processed to form the compound acetyl-CoA.
During this step of respiration, another molecule fo NADH is produced. |
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Term
Citric acid cycle (KREBS cycle) |
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Definition
Acetyl-CoA is oxidized to two molecules of CO2.
During this sequence of reactions, more ATP and NADH are produced and FAD is reduced to form another electron carrier, FADH2. |
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Term
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Definition
Electrons from NADH and FADH2 move through a series of proteins called an electron transport chain (ETC).
The potential energy released during these redox reactions is used to create a proton gradient across a membrane; the ensuing flow of protons back across the membrane is used to make ATP. |
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Term
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Definition
Any suite of reactions that produces ATP in an electron transport chain. |
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Definition
when an enzyme in a pathway is inhibited by the product of the reaction sequence.
The product molecule "feeds back" to stop the reaction sequence when the product is abundant. |
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Definition
Layers of sac-like structures within the interior of th mitochondrion |
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Term
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Definition
The region inside the inner membrane but outside the cristae. |
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Term
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Definition
Acts as a coenzyme by accepteing and then trasnferring an acetyl group (-COCH3) to a substrate.
Pyruvate reacts with CoA, through a series of steps, to produce acetyl CoA, which is later oxidized to CO2. |
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Term
Electron Transport Chain (ETC) |
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Definition
As electrons are passed from one protein to another in the chain, the energy released by the redox reactions is used to pump protons across the inner membrane of mitochondria.
After this proton gradient is established, a stream of protons through the enzyme ATP synthase makes part of the protein spin, driving the production of ATP from ADP and P_i. |
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Term
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Definition
A metabolic pathway that regenerates NAD+ from stockpiles of NADH; it allows gylcolysis to continue producing ATP in the absence of the electron acceptor required by the ETC.
Allows AT to be produced int eh absence of oxygen. In organisms that usually use oxygen as an electron acceptor, fermentation is an alternative mode of energy production when oxygen supplies temporarily run out. |
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Term
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Definition
a cell's entire complement of DNA |
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Term
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Definition
An enzyme that polymerizes deoxyribonucleotides to DNA; catalyzes DNA synthesis. |
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Term
deoxyribonucleoside triphosphates (dNTPs) |
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Definition
monomers that act as substrates in polymerization reactions. The N in dNTP stands for any of the four bases found in DNA: adenine, thymine, guanine, or cytosine. |
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Term
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Definition
A Y-shaped region where the parent-DNA double helix is split into two single strands, which are then copied. |
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Term
single-stranded DNA-binding proteins (SSBPs) |
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Definition
attach to the separated strands of DNA and prevent them from snapping back into a double helix, during replication. |
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Definition
an enzyme that cuts DNA, allows it to unwind, and rejoins it ahead of the advancing replication fork. |
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Term
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Definition
an enzyme that synthesizes a short stretch of ribonucleic acid (RNA) that acts as a primer for DNA polymerase.
Primase is a type of RNA polymerase - an enzyme that catalyzes the polymerization of ribonucleotides into RNA. Unlike DNA polymerase, primase does not require a primer. |
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Definition
an enzyme that catalyzes the formation of a phosphodiester bond between the adjacent fragments |
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Term
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Definition
region at the end of a linear chromosome |
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Term
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Definition
an enzyme that catalyzes the synthesis of DNA from an RNA template. |
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Term
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Definition
carry information from DNA to the site of protein synthesis |
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Term
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Definition
The process of copying hereditary information in DNA to RNA.
DNA is trasncribed to RNA, by RNA polymerase. |
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Definition
The process of using the information in nucleic acids to synthesize proteins.
Messenger RNA is translated to proteins, in ribosomes. |
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Term
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Definition
the rules that specify the relationship between a sequence of nucleotides in DNA or RNA and the sequence of amino acids in a protein. |
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Definition
a group of three bases that specifies a particular amino acid |
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Definition
Any permanent change in an organism's DNA. It is a modification in a cell's information archive - a change in its genotype. Mutations create new alleles. |
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Definition
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Term
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Definition
a point mutation that does not change the amino acid sequence of the gene |
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Term
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Definition
the strand that is read by the enzyme |
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Term
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Definition
DNA strand that is not read by the enzyme; sequence of nucleotides matches the RNA sequence that is transcribed from the template strand and codes for a polypeptide |
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Term
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Definition
sigma + RNA polymerase; "whole enzyme" |
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Term
downstream of transcription |
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Definition
DNA that is located in the direction RNA polymerase moves during transcription is said to be 'downstream' from the point of reference |
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Term
upstream of transcription |
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Definition
DNA located in the opposite direction RNA polymerase moves during transcription. |
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Term
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Definition
A unique sequence centered about 30 base pairs upstream of the transcription start site. |
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Term
basal transcription factors |
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Definition
Proteins that initiate eukaryotic transcription by binding to the appropriate promoter region in DNA.
Function is analogous to the function of the sigma proteins in bacteria, exept that many proteins are invoved, instead of one, and basal transcription factors are not part of a holoenzyme. |
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Term
elongation phase of transcription |
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Definition
1. The process by which messenger RNA lengthens during transcription
2. The process by which a polypeptide chain lengthens during transcription |
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Term
Termination phase of transcription |
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Definition
In most cases, transcription stops when RNA polymerase reaches a DNA sequence that functions as a transcription-termination signal |
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Term
primary transcription (pre-mRNA) |
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Definition
When a eukaryotic gene is transcribed, the product is an immature primary transcript, or pre-mRNA.
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Term
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Definition
The regions of eukaryotic genes that are part of the final mRNA |
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Term
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Definition
A region of a eukaryotic gene that is transcribed into RNA but is later removed, so it is not translated into a peptide or protein |
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Term
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Definition
As transcription proceeds, this proccess removes the introns from the growing RNA strand. |
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Term
small nuclear ribonucleoproteins (snRNPs) |
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Definition
In most cases, splicing is catalyzed by complexes of proteins and specialized RNAs, called small nuclear RNAs. These protein-plus-RNA complexes are known as snRNPs |
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Term
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Definition
In eukaryotes, a large, complex assembly of snRNPs that catalyzes removal of introns from primary RNA transcripts |
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Definition
As soon as the 5' end of a eukaryotic RNA emerges from RNA polyerase, enzymes add a structure called the 5' cap.
The cap consists of the molecule 7-methyl-guanylate and three phosphate groups. |
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Term
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Definition
An enzyme cleaves the 3' end of most RNAs once transcription is complete, and another enzyme adds a long row of 100-250 adenine nucleotides, not encoded on the DNA template strand. |
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Term
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Definition
the general term for any of the modicfications, such as splicing or poly(A) tail addition, needed to convert a primary transcript into a mature RNA. |
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Term
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Definition
A large complex structure that synthesizes proteins by using the genetic information encoded in messenger RNA strands.
Consists of two subunits, each compsed of ribosomal RNA and proteins. |
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Definition
A structure consisting of one messenger RNA molecule along with many attached ribosomes and their growing peptide strands. |
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Term
Transcripton/Translation in Bacteria |
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Definition
Transcription and translation can occur concurrently in bacteria because there is no nuclear envelope to separate the two processes.
Thus transcription and translation are physically connected. |
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Term
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Definition
One of a class of RNA molecules that have an anticodon at one end and an amino acid binding site at the other.
Each tRNA picks up a specific amino acid and binds to the corresponding codon in messenger RNA during transcription. |
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Term
aminoacyl tRNA synthetase |
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Definition
an enzyme that catalyzes the addition of a particular amino acid to its corresponding tRNA molecule |
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Term
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Definition
A transfer RNA molecule that is covalently bound to an amino acid |
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Term
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Definition
a set of three ribonucleotides that forms base paris with the mRNA codon |
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Term
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Definition
A RNA molecule that forms part of the structure of a ribosome |
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Term
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Definition
in a bacterial mRNA molecule, the sequence just upstream of the start codon to which a ribosome binds to initiate translation. |
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Term
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Definition
Proteins that mediate the initial interaction between the small subunit and the message |
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Term
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Definition
The process by which a ribosome moves down a messenger RNA molecule during translation |
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Term
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Definition
Proteins involved in the elongation phase of tranlation, assisting ribosomes in the synthesis of the growing peptide chain. |
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Term
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Definition
Proteins that can trigger termination of RNA translation when a ribosome reaches a stop codon. |
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Term
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Definition
Protein that can trigger termination of RNA translation when a ribosome reaches a stop codon.
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Term
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Definition
Occurs when regulatory proteins affect RNA polymerase's ability to bind to a promoter and initiate transcription
[image] |
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Term
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Definition
occurs when regulatory molecules alter the length of time an mRNA survives before it is degraded by ribonucleases, or affect translation intitiation, or affect elongation factors and other proteins during the translation process. |
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Term
post-translational control |
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Definition
Some proteins are manufactured in an inactive fomr and have to be activaated by chemical modification, such as the addition of a phosphate group. |
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Term
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Definition
genes that are transcribed all the time |
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Term
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Definition
a substrate in a reaction and it stimulates the expression of a specific gene or genes. |
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Definition
chemicals that damage DNA and increase mutation rates |
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Definition
a method of identifying bacterial colonies that have certain mutations by tranferring cells from each colony on a master plate to a second (replica) plate and observing their growth when exposed to different conditions |
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