Term
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Definition
| process by which Holliday junction can move down paired chromatids, forming more heteroduplex DNA |
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Term
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Definition
| Cre enzyme recognizes and cleaves loxP sites, can be used to make targeted recombination events, like turn a gene in a mouse on or off |
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Term
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Definition
| gives rise to recombinant gametes or nonparental meiotic products |
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Term
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Definition
| break across both strands of DNA |
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Term
| double-strand break repair |
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Definition
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Term
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Definition
| irregular 3:1 or 1:3 segregation, important outcome of HR, often associated w/ reciprocal crossover of flanking genes, sometimes incomplete |
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Term
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Definition
| selectable marker is inserted into coding region of a gene, thereby disrupting its activity |
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Term
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Definition
| disruption of gene activity by DNA transformation made by mitotic recombination, types: replacement and insertion |
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Term
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Definition
| all or part of coding sequence is deleted and replaced with selectable marker |
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Term
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Definition
| DNA that has two genotypes, with one or a few DNA mismatches |
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Term
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Definition
| crossed strand intermediate of HR in Holliday model, has four-way symmetry and moves through branch migration |
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Term
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Definition
| essential process for repairing DNA double strand breaks and for ensuring correct chromosome segregation in meiosis |
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Term
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Definition
| higher frequency, double-strand breaks catalyzed by Spo11, nonsister chromatids is preferred donor of information |
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Term
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Definition
| less frequent, sister chromatid is preferred donor of information |
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Term
| nonhomologous end-joining |
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Definition
| used to repair double-strand breaks where no homology or limited "microhomology" is present, Ku bridge |
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Term
| positive-negative selection |
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Definition
| positive: select marker (carrying antibiotic resistance) that disrupts gene of interest, negative: carries gene at end that makes cell sensitive to antibiotic. If precise target, will be resistive to both. |
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Term
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Definition
| strand exchange protein that binds DNA and promotes the early steps of strand exchange between homologous DNA sequences |
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Term
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Definition
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Term
| recombination signal sequences |
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Definition
| join V, D, J sequences, fromed from conserved heptamer and nonamer elements separated by spacer region of 12 or 23 nts |
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Term
| single-strand annealing (SSA) |
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Definition
| repairs double-strand breaks that occur between repeated sequences of the same orientation. A repeated sequence is deleted as ends are annealed to form 3'-OH ends |
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Term
| site-specific recombination |
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Definition
| used for immunoblobulin diversity and by transposable elements; exchange, insertion, or deletion of DNA segments at one or two defined DNA sequences |
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Term
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Definition
| promoted by RecA, binding of single-stranded DNA to homologous sequences, intermediate: D-loop |
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Term
| symmetric heteroduplex DNA |
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Definition
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Term
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Definition
| proteinaceous structure in which meiotic recombination occurs, forms along chromosome arms and serves as scaffold to connect interacting homologous chromosomes, needed for crossover formation |
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Term
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Definition
| heterozygous for knockout gene of interest |
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