Term
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Definition
| The purpose of containment is to reduce or prevent ____________ of laboratory workers, other persons, and the outside environment to potentially hazardous agents. |
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Term
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Definition
| For surface decontamination, 70% ethanol may be used, but the surface should be ______________with ethanol and the area allowed to dry by ___________________, because ethanol achieves its antimicrobial properties by desiccating (completely drying out) the infectious agent. |
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Term
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Definition
| Biological safety cabinets should be ________________ BEFORE AND AFTER each use. Potentially contaminated materials should be too before they are brought outside of the cabinet. |
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Term
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Definition
| Medical waste in red bags that have been autoclaved at an approved autoclave facility with a visible indication of decontamination, such as _______________ _____________, may be disposed of as non-regulated solid waste. |
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Term
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Definition
| Biohazardous and medical waste may not be stored for more than ___ days at room temperature or more than ____ days at 0 degree Celsius. |
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Term
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Definition
| Sharps include: needle syringes, ________________, razor blades, scalpels or anything else that is capable of puncturing human skin or a biohazard bag. |
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Term
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Definition
| Sharps containers must be disposed within ______ days of being filled. Never fill a sharps container more than _____ full in order to prevent injury. |
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Term
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Definition
| Non-_______________ broken glass or pipettes may be disposed of in glass disposal boxes in the regular trash as long as they are _________ closed before disposal. |
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Term
| pipetting, centrifugation, sonication |
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Definition
| Examples of procedures that may generate aerosols include ______________, ______________, tissue culture, ____________, blending of tissues and intranasal inoculation of animals . |
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Term
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Definition
| If aerosols may be generated, the work must be performed in a Class II biosafety cabinet. Class II biosafety cabinets are designed with inward air flow at a specified velocity to protect personnel, HEPA-filtered downward vertical laminar airflow for product protection and HEPA-filtered exhaust air for environmental protection. It is important to recognize that horizontal laminar flow hoods designed to protect items and your work within the hood from contamination are NOT Class II biosafety cabinets and would not provide protection from contamination to the ______________ or ______________. |
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Term
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Definition
| Many of infectious agents used at BSL-2 have _____________ available to protect personnel or infections can be readily treated with standard therapy. |
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Term
| containment, security, training |
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Definition
| Biosafety Level-3: consists of infectious agents which can be transmitted through aerosol exposure. BSL-3 requires additional _________________, ______________ and ___________requirements. |
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Term
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Definition
| “______________” refers to an organism in which foreign DNA has been introduced into its germ-line cells and will become an inherited characteristic of the progeny of that animal. |
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