Term
| What has the largest amount of DNA that can be cloned into vectors? |
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Definition
| YAC (yeast artificial chromosome) 1000 kb |
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Term
| What has the least amount of DNA that can be cloned in vectors? |
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Definition
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Term
| What are the most commonly used vectors? |
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Definition
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Term
What type of order is this in terms of DNA carrying ability?
Plasmid, bcateriophage, cosmid, P1 vector, YAC |
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Definition
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Term
| What commonly cloning vector is found in ecoR1 has antibiotic res. genes. and indep origin of rep (rep on its own) |
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Definition
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Term
| What are three things that Yeast Artificial Chromosome needs? |
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Definition
Telomere, centromere, and ARS (allows for indep DNA rep)
ARS stands for autonomous replication sequence |
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Term
| To test if host has recombinant DNA put in a media with antibiotic and then... |
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Definition
| if it is the only thing that grows then it worked! |
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Term
| Introduction of target into plasmid will change the characteristic of plasmid this is called? |
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Definition
Insertional Inactivation
(results in huge frameshift mutation whatever gene the foreign DNA is on no longer works |
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Term
| To make certain we could find a clone to make production of a protein we would use a? |
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Definition
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Term
| what library is used to study promoter sequence? |
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Definition
| Genomic library (promoter sequence would NOT BE EXPRESSED in the cDNA) |
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Term
What test can be used for
a. DNA finger printing (RFLP)
b. Genetic counseling, ex. Cystic fibrosis
c. DNA mapping
d. Sequence homology |
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Definition
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Term
| What test involves a transfer of DNA from a gel to a solid matrix. Gives info about presence or absence of a particular gene (DNA). Gives NO info whether the genes are expressed or not in a given tissue. |
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Definition
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Term
| What test involves transfer of RNA from a gel to a solid matrix and gives info about whether RNAs are present in a tissue. Often used to determine whether particular genes are expressed. Does NOT give info about whether the gene is present in other tissue. |
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Definition
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Term
| What type of test involves transfer of proteins from gel to solid matrix and gives info about expression of a given protein. LABELED ANTIBODIES are used as probes. |
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Definition
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Term
| If you wanted to find ribosomal DNA genes you would use... |
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Definition
| gDNA you're right! good job! |
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Term
| If you want to find a functional protein you would use? |
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Definition
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Term
| When you use total DNA this is called a? |
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Definition
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Term
To see if sequence is there the following tests use what?
Southern Blot
Western Blot
Northern Blot |
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Definition
Southern blot - DNA
Western blot - Protein
Northern Blot - RNA |
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Term
| Northern and southern blotting use? |
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Definition
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Term
| PCR is 3 stages resulting in a million base pairs of your gene of interest. What are the three steps? |
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Definition
1.Denature the double strand
2. Lower temp to anneal; allow base pairing
(add Taq polymerase and dNTPs, incubuate at 72 degrees C)
3. Raise temp to extend the primers |
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Term
| Sickle Cell gene is missing a cleavage site resulting in a... |
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Definition
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Term
| Restriction endonucleases are ? specific. |
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Definition
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Term
| Humans differ amonst each other in regards to their ?. This allows for CSI to pick right suspect even based on one hair. Also used in paternity testing |
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Definition
| VNTR (variable nucleotide tandem repeats) |
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Term
| What test can be used for breast cancer screening, prostate screening, and also result in genetic discrimination? |
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Definition
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Term
What can turn genes off by specifically binding to mRNA.
Use against over expressed gene in cancer cells |
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Definition
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