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Reagent Strip Testing Part 2
Remainder of Reagent Strip Testing
37
Medical
Undergraduate 2
02/29/2012

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Cards

Term

Bilirubin Principal of Test

Definition
diazo reaction
Term
Bilirubin Test Strip Impregnation
Definition

diazonium salt

 

buffer

Term
Bilirubin Test Methodology
Definition
bilirubin combines with diazonium salt in an acid medium to produce azobilirubin, a colored compound
Term
Free or Unconjugated Bilirubin
Definition
non soluble bilirubin transported by MPS to liver
Term
Conjugated Bilirubin
Definition
bilirubin becomes soluble by conjugation with glucoronic acid
Term
Which type of bilirubin can be eliminated by kidney or liver?
Definition
Only conjugated bilirubin
Term
Urobilinogen Conversion
Definition
bilirubin is converted by bacteria in the small intestine into urobilinogen
Term
Normal Bilirubin Result
Definition
negative
Term
Bilirubin Test Sensitivity
Definition
0.5 - 1 mg/dL
Term
Bilirubin Test False Negative
Definition

exposure to light

 

ascorbic acid

 

elevated nitrites

Term
Bilirubin Test False Positive
Definition
color interfering medications
Term
Urobilinogen Testing Principle
Definition
diazo reaction
Term
Urobilinogen Test Strip Impregnation
Definition

diazonium salt

 

buffer

Term
Urobilinogen Test Methodology
Definition
urobilinogen reachs with dazonium salt to form a red azo dye
Term
Urobilinogen Normal Test Result
Definition
1 mg/dL
Term
Urobilinogen Test Sensitivity
Definition
0.4 mg/dL
Term
Urobilinogen Test False Negative
Definition

exposure to light

 

complete absence of urobilinogen

Term
Urobilinogen Test False Positive
Definition
highly colored pigments
Term
Leukocytes Test Principle
Definition

ester is cleaved by leukocyte esterase to produce an aromatic compound

 

aromatic compound reacts with diazonium salt to produce purple color

Term
Leukocyte Test Normal Result
Definition

negative

 

Term
Leukocyte Test Sensitivity
Definition
5 - 10 WBC/HPF
Term
Leukocyte Specificity
Definition
esterase
Term
Leukocyte Test False Reactions
Definition

trichonomas

 

contain esterase

 

aminoglycosides, proteinuria

Term
Nitrites Test Principle
Definition

"griess test"

nitrites + p-arsanillic acid yield diazonium salt

 

diazonium salt + aromatic compound yield azo dye

 

azo dye changes from white to pink

Term
Nitrites Normal Result
Definition
negative
Term
Nitrites Test Sensitivity
Definition
0.03 - 0.06 mg/dL
Term
Nitrites Test Flase Positive
Definition
red color urine
Term
Nitrites Test False Negative
Definition

insufficient time in bladder

 

ascorbic acid

 

insufficient dietary nitrites

Term
Specific Gravity Test Principle
Definition

partially disassociated polyelectrolyte releases H+ in the presence of positive ions

 

free H+ cases indicator to change to its acid form

Term
Specific Gravity Normal Result
Definition
1.003 - 1.030
Term
Specific Gravity Test Sensitivity
Definition
1.000 - 1.030 in .005 units
Term
Specific Gravity Test Specificity
Definition
positive ions
Term
Specific Gravity Test False Positive
Definition
increased urine
Term
Specific Gravity Test False Negative
Definition
alkaline urine
Term
Specific Gravity Definition
Definition
evalulated the weight of solids/molecules in water
Term
Osmolality Definition
Definition
measures the number of molecules in water
Term
Principles of Fluorescent Flow Cytometry
Definition

LED emits a specific wavelength of light onto the test strip

colored product absorbs the light, darker the test pad the less reflected light

detector captures the reflected light and converts it into an electronic signal that produces a result

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