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Pure Culture Techniques
Pure Culture Techniques
14
Microbiology
Undergraduate 2
10/23/2011

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Cards

Term
Bacteria exist in natural environments as MIXED CULTURES?
Definition
True!
Term
Who is the father of medical microbiology?
Definition
Robert Koch
Term
What does a pure culture contain?
Definition
It contains only a single kind of an organism.
Term
What does a mixed culture contain?
Definition
More than one kind of organism.
Term
What does a contaminated culture contain?
Definition
The desired organism + unwanted organisms
Term
What are 2 procedures for obtaining pure cultures?
Definition

1. streak plate

2. pour plate

Term
What is the advantage of pour-plate method?
Definition
Requires less skill
Term
What is the disadvantage of the pour-plate method?
Definition
Requires more media, tubes and plates.
Term
Why would a culture of S. marcescens not be red if inoculated at a temperature higher than 25 C?
Definition
Because higher temperatures inhibit the formation of the organism's red pigment.
Term
What kind of gram is S. marcescens? Shape?
Definition
genative short rod
Term
What kind of gram is M. luteus? Shape?
Definition
Positive coccus.
Term
What is a colony?
Definition
Cells that result from one parents cell.
Term
Why is dilution a necessary prt of pure culture preparation?
Definition
For getting isolated colonies.
Term
Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled to 50 C?
Definition
In order to avoid condensation of moisture on the cover.
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