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Principles of Microbiology
Micro, Lab Practical 1
13
Microbiology
Undergraduate 3
04/27/2010

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Cards

Term
what techniques were used in the lab to isolate colonies?
Definition
Streak plate, smear plate
Term
what is the difference between the two methods of isolation?
Definition
streak plate you drag the bacteria through different quadrants, minimizing the amount you drag each time through a new quadrant, resulting in isolated colonies.  smear plate, dilute the bacteria, then smear on the agar.
Term
what is a selective media
Definition
a substance that does/doesnt allow one or more bacteria to grow
Term
what is a differential media?
Definition
something that distinguishes between different bacteria
Term
what makes msa, emb, and mackonkey agar selective?
Definition
salt, eosin y and methelyne blue dye, bile salts and crystal violet dye
Term
what does msa, emb, and mackonkey agar select for?
Definition
staphylococcus, gram -, gram -
Term
what makes msa, emb, and mackonkey agar differentail?
Definition
mannitol, lactose and sucrose, lactose
Term
what does msa, emb, and mackonkey agar differentiate between?
Definition
s. aureus, fecal coliform, enterobacteriaceae
Term
what pH indicator is used in msa, emb, and mackonkey agar?
Definition
phenol red, eosin y and methelyne blue, neutral red
Term
what causes the indicator to change color?
Definition
fermentation
Term
what is the Kerby Bauer Test used for?
Definition
to find out which antibiotics are resistant/suseptible to which bacteria
Term
what is an antibiotic?
Definition
a substance that inhibits growth or kills a bacteria
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