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principle of inflammation & immune system
pharmacology exam 3
120
Pharmacology
Graduate
04/03/2012

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Cards

Term
Which system is the first responder to an offending agent?
Definition
Innate immune system 
Term
How does the innate immune system recognize antigens?
Definition

By pattern recognition receptors:

- Toll-like receptors (TLRs) recognise bacterial LPs

- Dectin-1 recognizes fungal Beta-glucans 

Term
T/F: innate immune system had memory and the response will be the same upon repeated exposure?
Definition
False, innate immune system lack memory
Term
How is the adaptive immune system stimulated?
Definition
It neutralize the infectious agent or engulf----phagocytosis of an offending agent digest macromelecule into fragments (antigens)---antigens are displayed on the surface of antigen presenting cells---antigen presenting cells stimulate the adaptive immune system 
Term
What is the major arm of innate immune system?
Definition
Granulocytes, contain chemical mediators that are released upon stimulation (neutrophils, eosinophils, basophils, and mast cells)
Term
What complex is involved in the adaptive immunity?
Definition
Major histocompatibility complex (MHC) proteins at the cell surface
Term
T/F: B cells and T cells are part of the adaptive immunity?
Definition

True, B cells fction as antigen presenting cells, they synthesize and secrete antibodies

T cells: Cytotoxic T cells (Tc)=cellular adaptive immunity 

Helper T cells (Th)= regulate adaptive responses (Th1,Th2)

Term
What are the differences between innate immunity and acquired immunity?
Definition

Innate immunity: immediate onset of action, recognizes antigen through receptors, cell types: macrophages, neutrophils, mast cells, natural killer cells, complement interferon

Acuired immunity: it takes days to wks for onset of action, recognize antigen through unique antigen specific receptors (T-cell, B-cell receptor), cell types: antigen presenting cells, T & B lymphocytes

Term
What is the mechanism of action of antigen presenting cells?
Definition
They process the macromolecules of invading agent into fragments, display them to T cells and provide costimulatory signals for T cell activation 
Term
What two cells function as antigen presenting cells (APC)?
Definition

- Macrophages: as APC (adapive immunity), and as phagocytosis of cellular debris & foreign particles (innate immunity), involved in chronic inflammation

- Dentritic cells: engulf antigens fron one location, then travel & present the antigen to T cells at different location

Term
Where does the MHC class I protein bind with degraded protein fragment (antigen)?
Definition
In the ER 
Term
T/F: MHC I is tranported to the cell surface, where it serves as a fingerprint for all proteins expressed by that cell (self)?
Definition
True 
Term
T/F: MHC I has a CD4 binding site that ensures that the interaction occurs only with cytotoxic T cells?
Definition
False: has CD8 binding site that do that 
Term
T/F: all nucleated human cells express MHC I proteins?
Definition
True 
Term
T/F: MHC II is transported to the cell surface, where it serves as fingerprint for all self antigen?
Definition

False, serves as fingerprint for nonself antigen 

MHC I serves as fingerprint for self antigen 

Term
T/F: MHC II has CD4 binding site that ensure that the interaction occurs only with helper T cells?
Definition
True 
Term
T/F:cytotoxic T cells (Tc) express T-cell receptors (TCR) and CD4. The TCR indentifies nonself antigens bound the MHC proteins, and CD4 ensures that Tc cells interact only with cells on MHC I proteins?
Definition
False, Tc express TCR and CD8, and CD8 ensures Tc interaction with MHC I
Term
T/F: Th express TCR and CD4, and CD4 binds to MHC II?
Definition
True
Term
T/F: Th cells interact only with cells expressing MHC II?
Definition
True
Term
Interaction of CD28 on Th cells with which proteins is required for Th activation?
Definition

with B7 (costimulatory molecule) family on APC

This is a costimulatory signal, required for response of Tcells 

Term
What is secreted by an activated Th cells?
Definition
IL-2, which expressed IL-2 receptor and stimulates further Th-cell proliferation and activation 
Term
T/F: IL-2 and other cytokines secreted by Th cell activate not only Th cells, but also Tc and B cells?
Definition
True 
Term
What is an acute inflammation?
Definition

Initial response to tissue injury/infection, involves the innate immunity (mostly neutrophyls accumulate)

Its a self-limited process 

Term

What is released in an acute inflammation?

Definition

Autacoids (histamine, bradykinin, prostaglandins, leukotrienes), cytokines and chemokines 

Term
What is a chronic inflammation?
Definition

Sustained and inappropriate response to inflammatory stimulus (pathogen, self-antigens), predominant accumulation of macrophages, involves the adaptive immunity (lymphocytes)

implicated in atoimmune disease and organ plant rejections 

Term
What are membrane derived lipid mediators?
Definition
Prostaglandins, leukotrienes, and platelet activation factor (PAF)
Term
What is produced through degranulation?
Definition
Histamine, heparin, serotonin, proteases 
Term
Where is histamine synthesized and stored?
Definition
in granules of mast cells and basophils
Term
What is the function of histamine?
Definition

Act as an initiator of inflammatory process

It's a vasocative amine-dilates arterioles and postcapillary venules, constriction of veins 

Term
T/F: complement are activated in response to injury, adaptive immunity?
Definition
False, innate immunity 
Term
What is complement?
Definition

A system of serine proteases 

C3a, C3b 

Term
How does complement trigger inflammatory reaction?
Definition
upon activation they attract phagocytes to the area. phagocyte engulf and digest dead cells and bacteria 
Term
Which cells are target for pharmacological interventions?
Definition
Cytokines 
Term
Which chemical mediators act as vasodilators?
Definition
Prostaglandins (PGI2, PGE1, PGE2, PGD2), nitric oxide (NO)
Term
Which chemical mediators increase vascular permeability?
Definition

- Histmine

- C3a, C5a

- Bradykinin 

- Leukotrienes (LTC4, LTD4, LTE4)

- Platelet-activating factor

- Substance-P

Term
Which chemical mediators act as chemotaxis and leukocyte activators? 
Definition

-C3a, C5a

-LTB4

-Lipoxins

-Bacterial products

Term
Which chemical mediators produce tissue damage?
Definition

- Neutrophil

- macrophages lysosomal products

- Oxygen radicals

- NO

Term
Which chemical mediators cause pain?
Definition


- PGE2

- PGI2

- Bradykinin

- CGRP

Term
Which chemical mediators produce fever?
Definition

- IL-1

- IL-6

- TNF-alfa 

Term
In what disease states does Eiconanoids act?
Definition

- Inflammatory conditions

- Autoimmune dz

- Asthama

- Glomerulonephritis

- Cancer

- Sleep disorders

- Alzheimer's dz 

Term
What are eicosanoids?
Definition

Arachidonic acid derived:

Prostaglandin

Thromboxanes

Leukotrienes 

act as proinflammatory and anti-inlfammatory

Term
T/F: arachidonic acid is part of membrane phospholipids?
Definition
True 
Term
How is arachidonic acid released?
Definition
Upon hydrolysis of membrane phosoholipids by PLA2
Term
T/F: production of a specific PG is independent of cell-specific enzymes, and PGs have long t1/2?
Definition
False, PG are dependent on cell-specific enzymes, and have short t1/2
Term
In what organs is Cox-1 expressed?
Definition
Gastric mucosa, kidneys, platelets and endothelial cells 
Term
T/F: Cox-1 undergo induction anf is responsible of housekeeping functions: vascular homeostasis, regulation of renal and GI blood flow, renal function?
Definition

False, no inductions

2nd part is true

Term
Cox-2 is inducible by which inflammatory cells?
Definition
Proinflammatory cytokines: TNF-a, IL-1, IL2, EGF, IFN-g
Term

Which of the followings are pathophysiological implications of Cox-1?

I) Housekeeping fct, renal fct

II) Fever

III) Intestinal mucosal proliferation

IV) platelet fct

V) Anti-thrombogenesis


A) I, II, & V are correct

B) only I is correct

C) I, III, IV & V are correct

d) All are correct 

Definition
C) I,III, IV & V are correct
Term
What chemical structure is shared by protaglandins?
Definition
Prostanoid 
Term

Which protaglandin act as vasodilator, inhibition of platelet activation, sleep, and alzeimer's dz?

 a) PGE2

b)PGD2

c) PGF2a

d) PGI2

Definition
b)PGD2, found in mast cells and neurons
Term

Which prostaglandin act as a protector of gastric mucosa, vasodialtion, hyperalgesia, cytoprotective (GI), acid secretion (GI), mucus formation?

a) PGE2

b)PGF2a

c)PGD2

d) PGI2

Definition

a) PGE2, found in many tissues,

macrophages and mast cells 

Term

T/F: TXA2 act ad vasoconstrictor, bronchoconstrictor and platelet aggregation?

 

Definition
True, found in platelets
Term
Which prostaglandin act as vasodilator and inhibition of platelet activation?
Definition
PGI2 (prostacyclin), found in endothelial cells 
Term

What are the major actions of PGF2a?

a) vascular tone

b) Reproductive physiology

c) Bronchoconstriction

d) all of the above

Definition
d) all of the above, found in vascular smooth muscles and uterine smooth muscles 
Term
How are prostaglandins released from cells?
Definition
Released from cells in response to chemical stiuli or physical trauma to perform local action
Term
T/F: Prostaglandins ptomote tissue inflammtion by stimulating inflammatory cell chemotaxis, causing vasoconstriction and increasing capillary permeability and edema?
Definition
False, causing vasodilation
Term

Which ones below are relatively selective Cox-2 inhibitors?

a)Nabumetone

b) Etodolac

c) Meloxicam

d) all of the above 

Definition
d) all of the above are 
Term
Which cox inhibitors are selective cox-2 inhibitors?
Definition
Celecoxib (celebrex), rofecoxib (vioxx), and valdecoxib (bextra)
Term

Which NSAID is the most potent?

a)Aspirin

b) Ketorolac

c) Naproxen

d) Indomethacin

Definition
b) Ketorolac is most potent 
Term

Which NSAID is least potent?

a) Keterolac

b) Celecoxib

c) Aspirin

d) Ibuprofen

Definition
c) Aspirin 
Term
What is the therapeutic effect of NSAIDs?
Definition

 

Inhibition of PG synthesis 

Term
T/F: Inhibition of PG synthase in gastric mucosa results in GI damage (dyspepsia, gastritis)?
Definition
True 
Term
What are common ADE of NSAIDs?
Definition

- Gastritis and peptic ulcer w/bleeding

- Acute renal failure 

- Sodium and water retention and edema

-Analgesic nephropathy

-Prolomgation of gestation and inhibition of labor

- GIT bleeding and perforation 

- PG inhibition-mediated hypersensitivity

- Platelet dysfunction

Term
T/F: NSAIDs decrease PG-mediated capillary permeability resulting reduction in edema, swelling and local warmth?
Definition
True
Term
T/F:Acetaminophen does decrease swelling?
Definition
False, does not 
Term
T/F: reversal of only the peripheral not central sensitization of pain receptos contribute to the analgesic activity of NSAIDs?
Definition
False, reversal of both peripheral and central sensitization 
Term
What is the pain (nociception) pathway?
Definition

1) Thermal, mechanical and chemical---nearby cell damage

2) Nociceptor activation

3) CGRP and substance-P released by activated nociceptors

4) Blood vessel dilation and mast cell degranulation

Term
What is the cause of fever?
Definition
Fever results due to altered hypothalamic thermoregulatory mechanisms 
Term
T/F: All NSAIDs relieve fever by inhibiting PG synthesis in the hypothalamus, but they are not capable of reducing body temperature below normal?
Definition
True 
Term
T/F: Basal temperature is affected by NSAIDs?
Definition
Fasle, its unaffected by NSAIDs
Term
T/F: Aspirin inhibits platelet aggregation and is used to prevent and treat arterial thromboembolic disorders?
Definition
True
Term
T/F: Traditional NSAIDs are thought to afford cardioprotection (short t1/2, reversibility of binding w/ COX enzyme)?
Definition
False, are not thought to do so, however Naproxen is an exception (~10% reduction of MI compared to 25% with low dose aspirin)(epidimiologic studies)
Term
What are the most important prostaglandins affecting platelet aggregation?
Definition
Prostacyclin PGI2 and TXA2
Term
T/F: PGI2 synthesized by vascular endothelial cells and promotes platelet aggregation?
Definition

False, inhibits platelet aggregation 

TXA2 promotes platelet aggregation

Term
T/F: Cox-1 is the predominant COX isoform in platelets?
Definition
True 
Term
T/F: endothelial cells express only Cox-2?
Definition
False, express both Cox-1 and Cox-2 
Term
What is the mechanism of aspirin-induced cardioprotection?
Definition
Low dose aspirin inhibits Cox-1 and Cox-2 (more Cox-1), therefore inhibit conversion of PGG2 to PGH2. (no more PGI2 and TXA2 formation)
Term
Why do we use low dose of aspirin?
Definition
Because low dose aspirin selectively inhibit synthesis of TXA2 without affecting protacyclin
Term
T/F: Like other NSAIDs aspirin reversibly inhibits cyclooxygenase enzyme (Cox-1) that catalyzes an early step on TXA2 synthesis?
Definition
False, unlike other NSAIDs aspirin irreversibly inhibits Cox-1
Term
How do NSAIDs cause gastric damage (ulceration)?
Definition
By inhibiting PG synthesis and blocking Cox-1 enzyme in GI (can occur w/ PO, IV and transdermal administration of NSAIDs)
Term
What is misoprostol?
Definition
It is a PGE1 analog has protective effects on GI, may give w/ NSAIDs
Term
T/F: PGE2 and PGF2-a induce uterine contraction?
Definition
Tru, therefore NSAIDs prolong labor by inhibiting PGE2 and PGF2-a production
Term
Use of NSAIDs in latre pregnancy may increase risk of postpartum hemorrhage. the patency of the doctus arteriosus (required for fetal circulation(aorta--lung)) is maintained by PGE2. NSAIDs during late pregnancy/labor induce prematue closure, thus impaired fetal circulation and cardiopulmonary issues
Definition
Term
How does patent ductus arteriosus PDA develop?
Definition
At birth, failure of ductus arteriosus to close results in PDA which can lead to pulmonary HTN, CHF, anbd cardiac arrythmias to neonate
Term
What can be used to treat PDA?
Definition
NSAIDs: indomethacin or ibuprofen injection can be used to treat PDA
Term
T/F: chronic uses of high doses of NSAIDs may cause analgesic nephropathy (slowly progressive renal failure)?
Definition
True 
Term
T/F: regular use of NSAID has been shown to increase risk of developing colorectal cancer (especially Cox-2 selective NSAIDs)?
Definition

False, decrease risk 

aspirin showed--50% reduction in risk of colon cancer

Term
T/F: Recent studies have shown that NSAIDs can delay or slow the progress of alzheimer's dz?
Definition
True 
Term
What are drug interactions with NSAIDs?
Definition

- ACE inhibitors (decrease effectiveness, increase risk of bradycardia and syncope in elderly and HTN, diabetes and heart pts)

- Cortocosteroids (increase frequency and severity of GI ulceration)

- Warfarin (increase plasma level, increase risk of bleeding)

- increase toxicity with methotrexate, warfarin, sulfonylurea (display from protein binding)

Term
What amount of aspirin will cause tinnutis and hearing loss?
Definition
3-6 g 
Term
what amount of aspirin is lethal?
Definition

4 g in children

20-25 g in adults 

Term
How is salicylism manifested?
Definition

Vomiting, sweating, tinnitus, dizziness, hallucination and convulsions 

respiratory alkalosis and decreased plasma buffering capacity 

Term
T/F: at doses>4g/d, salicylates compete with uric acid secretion, causing increased UA retention in blood?
Definition

False, <2g/d will do that

>4g/d will block UA reabsorption--increased UA excretion 

Term
At what doses salicylates will not affect UA excretion?
Definition
2-3 g/d 
Term
What are drug interactions with salicylates?
Definition

- Warfarin: risk of bleeding

- Aspirin toxicity when used w/ acididying drugs (ascorbic acid)

- Glucocorticoids: risk GI ulcers

- Ethanol: gastric erosion

Term
T/F: Diflunisal (Dolobid) used for osteoarthritis musculoskeletal strains or sprains is 3-4 * more potent than aspirin because, of peripheral effect?
Definition
True 
Term
T/F: diflunisal has antipyretic effect?
Definition
False, does not because has low access to CNS hypothalamus 
Term
What are the advantage of diflunisal?
Definition

- No auditory SE,

- Less intense GI effects

- Not metabolized to salicylate (less SE)

Note: has cross sensitivity with other NSAIDs

Term
T/F: Inhibition of cox-2 causes platelet activation and aggregation and vasodilation leading to increased tisk of MI, heart attack, stroke and thrombosis?
Definition
False everything is correct but vasodilation it causes vasoconstriction 
Term
T/F: Cox-2 inhibitors alter the vascular homeostasis by removing the cardioprotective effects of PGI2, leaving the atherogenic properties of TXA2 intact?
Definition
True
Term
T/F: Acetaminophen selectively inhibit Cox-3 in the CNS?
Definition
True 
Term
Which leukotrienes are consiodered as bronchoconstrictors?
Definition
LTC4, LTD4 and LTE4 known as cysteinyl leukotrienes
Term
T/F: zafirlukast and montelukast are slective competitive antagonists for cy-LT1 receptors?
Definition
True 
Term
What are ADE of zafirlukast?
Definition

Eosinophilia and vasculitis

DI: increase prothrombin time w/ warfarin 

metabolized by CYP2C9 

Term
MOA of zafirlukast?
Definition
Inhibit influx of basophils and lymphocytes into the airways (LTC4, LTD4, LTE4)
Term
T/F: montelukast has DI with warfarin?
Definition
False, no interaction
Term
What is the MOA of colchicine?
Definition

Inhibit microtuble assembly

- Decrease release of chemotactic factors by activated neutrophils

- Decrease histamine release from mast cells

- Decrease neutrophil motility and adhesion

Term
What are ADE of colchicine?
Definition

- Diarrhea, N/V, abdm pain

- Myelosuppression

- Neuromyopathy

Term
DI with colchicine?
Definition

Cyclosporine, tacrolimus, or verapamin increase plasma levels of colchicine (inhibit excretion of colchicine from bile, C & T from urine)

CYP3A4 substrate (cimetidine) increase colchicine plasma levels and toxicity 

Term
What are ADE of allopurinol?
Definition

Rash

Nephritis

Hepatitis

Kidney stones 

Term
Important DI w/ allopurinol?
Definition
Azathioprine and 6-mercaptopurine 
Term
T/F: When administered with allopurinol, the dose of 6-mercaptopurin or azathioprine should be reduced?
Definition
True 
Term
ADE of Rasburicase?
Definition

Hemolysis (G6PD-deficient pts)

Methemoglobinemia

Acute renal failure

Anaphylaxis

GI problems

Fever

HD, etc.. 

Term
T/F: Probenecid inhibits uric acid reabsorption by enhancing URATE-1 transporter?
Definition
False, by inhibiting URATE-1 transporter 
Term
Hematologic toxicity is important with which Uricosuric agent?
Definition
Sulfinpyrazone 
Term
T/F: Low dose aspirin antagonize probenecid action?
Definition
True 
Term
Which uricosuric agent has antiplatelet activity, thus caution w/ othe anticoagulan or antiplatelets)?
Definition
Sulfinpyrazone 
Term
DI of uricosuric agents?
Definition
Delays excretion of penicilin, nitrofurantoi and other anionic compounds 
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