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Practical 4
Study Cards for Bio 1 lab practical 4
97
Biology
Undergraduate 1
04/28/2010

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Term

Asexual Reproduction

Definition

involves only one individual. It is useful when environmental conditions are stable and the organism is well adapted.

ie: clone

Term
Reproduction
Definition
All organisms make more individuals similar to themselves
Term
Budding
Definition
one or several cells will break off the parent and become a new individual.
Term
Bulbs - Rhizomes - Stolons
Definition
all examples of asexual reproduction in plants.
Term
Sexual Reproduction
Definition
occurs in multicellular eukaryotes in specialized cells called sex cells.
Term
Gametes
Definition
These cells undergo a process in which the original chromosome number is reduced by half in new cells.
Term
Homologous Chromosome
Definition
Chromosome pairs that carry the smae genes at the same locations within the DNA molecules
Term
Diploid
Definition
There are two copies of every chromosome
Term
Haploid
Definition
special cells must be created that have half the number of chromosomes - so that when two of these special cells join together, we get back the full number.
Term
Spermatogenesis
Definition
Sperm formation
Term
Oogenesis
Definition
ovum formation
Term
genes
Definition
code for synthesis of enzymes that regulate cell reactions to determine a specific cell activity or appearance commonly called a character
Term
alleles
Definition
the genetic variations that produce traits 
Term
Polygenic inheritance
Definition
two or more genes work together to produce a specific outcome
Term
genotype
Definition
differences between the actual allels
Term
Phenotype
Definition
the physical expression of that genotype
Term
monohybrid cross
Definition
an individual that is homozygous dominant for a gene mates with an individual that is homozygous recessive
Term
duhybrid cross
Definition
involves parents that are homozygous (one parent dominant on parent recessive) for two differences genes.
Term
Three differences between Mitosis and Meiosis?
Definition

1. cells pass through each phase twice=4 cells

2. Dna replication only happens once but the cells divide twice so the cells are haploids.

3. synapsis and crossing over

Term
Synapsis
Definition
is when the two sets of sister chromatids from one homologous pair of chromosomes get bound together and intertwine.
Term
crossing over
Definition
happens after synapsis. is the swapping of chromosome pieces between the chromatids. Since this produces chromosomes with new gene combinations, crossing over increases genetic variability.
Term
Where and why meiosis takes place?significant to sexual reproduction?
Definition
occurs in multicellular eukaryotes in specialized cells called sex cells.Sexual production provides important genetic variability within a species that will allow tolerance to a wider variety of environmental conditions.
Term
differences between spermatogenesis and oogenesis.
Definition

-(sperm) in testes:produces four haploid sperm per starting cells

-(ova) in ovaries: cytokinesis is uneven,one large and three small cells are produced. three smaller ones are called polar bodies

Term

purple over____

smooth over____

Definition

yellow

wrinkled

Term
polygenic
Definition
Traits in humans that are determined by more than one gene on different chromosomes. ie: height, body weight, intelligence, skin color.
Term
Mendel's 4 discoveries
Definition

1. there are alternative forms of genes for each trait

2. for each inherited trait a diploid organism receives two genes.

3. law of segregation: a gamete carries only one allele because homologous chromosomes separate during meiosis

4. for those traits inherited by complete dominance allele of the pair is usually expressed while the other is not (dominant and recessive)

Term
Law of independent Assortment
Definition
if the genes are located on different chromosomes the inheritance of one will not affect the inheritance of the other.
Term
Population genetics
Definition
is the study of the changes in genotype and allele frequencies that occur in natural populations
Term
gene pool
Definition
the sum of genetic variability for that species 
Term
allele frequencies
Definition

p = the frequency of dominant alleles

q = the frequency of recessive alleles

p^2 +2pq+q^2=1

(p+q)^2=1 (binomial)

Term
Equalibrium
Definition
the freq. of the dominant allele and recessive allele remained unchanged generation after generation
Term
Hardy-Weinberg (H-W) equilibrium theory
Definition
is a mathematical principle that states that allelic and genotypic freqs. in a population remain constant unless acted upon by forces other than genetic recombination and the Law of Segregation
Term
Genotype freq.
Definition

p^2 = the freq. of homozygous dominant individuals

2pq = the freq. of herozygotes

q^2 = the freq. of homozygous recessive individuals

 

Term
H-W requirements(6)
Definition

1. Large population size

2. Panmixis

3.no natural selection

4. no migration

5. no mutation

6. no genetic drift

Term
panmixis
Definition
this means that individuals in a population do not select their mates. mate at random. equal likelihood of all possible combinations of alleles.
Term
Natural selection
Definition
is a term that refers to the effects of an environment on a species.
Term
mutation
Definition
any alteration to the nucleotide sequence in a gene. Mutations are a prime means for adaptation in response to environment.
Term
Genetic drift
Definition
is a concept that refers to random changes in allele frequencies that may result from processes such as founder effect or bottleneck effect.
Term
founder effect
Definition
the reproductive isolation of some portion of an existing population. if the survive in the new environment the portion that are isolated are colonists or founders in the new location.
Term
bottleneck effect
Definition
a catastrophic decline in the population size that may be caused by an unusual abiotic factor, such as a hurricane or tornado that kills off most of the population. the populatiuon remnant are called survivors.
Term
(equation)p=
Definition
#RR + 1/2# of Rr / total # individuals
Term
(equation) q =
Definition
#rr + 1/2 #Rr / total # individuals
Term
role of RNA and ribosomes
Definition
make copies of individual instructions within the DNA and to carry out the instructions
Term
Similarities in DNA and RNA
Definition

both are built from nucleotides:

5 carbon sugar (pentose)

phosphate functional grooup

nitrogen containing ring molecule called a nitrogenous base

Term
Differences between DNA and RNA
Definition

1. DNA has a double helix. RNA is single stranded

2. DNA contains pentose sugar deoxyribose. RNA contains only ribose

3. DNA contains nitrogenous bases Adenine, Guanine, Cytosine, and Thymine. RNA contains A, G, C and Uracil

Term
Purines
Definition
double ringed molecules: Adenine and Guanine
Term
Pyrimidines
Definition
Single ringed nitrogenous base Cytosine, Thymine, Uracil
Term
DNA Synthesis
Definition
During S phase each DNA molecule is EXACTLY duplicated
Term
Replication requirements (3)
Definition

1. Complementary Base Pairing

2. Anti-Parallel arrangement of strands

3. Semi-conservative nature of synthesis

Term
Complementary Base Pairing
Definition
Either single strand of DNA has any conservative sequence of the four N bases
Term
Chargaff's Rule
Definition

A=T

G=C

Term
Anti Parallel arrangement of strands
Definition

in order for bases to line up so that they can bond across the strands.

3' and 5' are in opposite directions.

5' = phosphate functional group

3'=hydroxyl functional group

 

Term
Semi-Conservative nature synthesis
Definition
in order to replicate any DNA molecule the helix only needs to be "unzipped" by breaking the H bonds at the base pairs
Term
DNA polymerase
Definition

DNA replication occurs at multiple locations simultaneously and works in both directions along the strands by the help of this enzyme.

Polymerase can only move from 3' towards 5'

Term
replication bubble
Definition
when the strands separate they form an opening 
Term
Initiation sites
Definition
series of bubbles that open all along the DNA 
Term
replication Fork
Definition
what does the "unzipping"
Term
Leading Strand
Definition

the portions of the strands that are aligned

3'->5' AWAY from the initiation site and TOWARDS the Fork

Term
Lagging Strand
Definition

the portions of the strands that are aligned

3'->5' TOWARDS the initiation site and AWAY from the forks

Term
Types of RNA
Definition

1. Ribosomal RNA (rRNA)

2. Transfer RNA (tRNA)

3. Messenger RNA (mRNA)

Term
mRNA
Definition
these are highly coiled strands of RNA found in ribosomes where protein synthesis occurs.
Term
tRNA
Definition
these are found as free molecules in the cytoplasm. function is to bring amino acids to the ribosome and transfer them to the ribosome to be added to the polypeptide being synthesized.
Term
mRNA
Definition
is created in the nucleus or cytoplasm as a copy of a gene.
Term
transcription 
Definition
mRNA formation process
Term
RNA Polymerase
Definition
copies one gene from one strand of DNA.
Term
Promoter and terminator regions
Definition
on the DNA to tell the RNA polymerase where to start and stop copying of the gene
Term
Translation
Definition
the process of converting the information in the mRNA which is formed by specific base sequences, into a polypeptide chain or protein=aka protein synthesis
Term
codons
Definition
information in the bases is read in these sets of tripilets
Term
AUG
Definition

first codon in mRNA

signifies the start of protein synthesis

Term
anticodon
Definition
correct amino acid is brought to the ribosome by tRNA which has an exposed three base sequence
Term
peptide bond
Definition
binds the amino acids at the A and P sites
Term
Developmental Processes (4)
Definition

1. Fertilization

2.Cleavage

3.Gastrulation

4. Organogenesis

Term
Fertilization
Definition
penetration of the sperm into the ovum and fusion of haploid gamete nuclei to form a single celled diploid zygote.
Term
Cleavage
Definition
rapid series of mitoses and cytokineses resulting in increasing number of progressively smaller cells such that the overall embryo size in unchanged.
Term
stages of cleavage
Definition
zygote=2 cell stage=4 cell stage=8 cell stage=morula=blastula
Term
blastomeres
Definition
individual cells
Term
morula
Definition
solid ball of 16-32 blastomeres.
Term
Blastula
Definition
contains between 64 and more than 2000 blastomeres and forms a hollow ball with the hollow center(blastocoel) filled with liquid
Term
Yolk Concentration (3)
Definition

1. Microlecithal: very little yolk present

2. Mesolecithal: moderate amount of yolk

3. Macrolecithal: large amount of yolk

Term
Yolk distribution (3)
Definition

1. isolecithal

2. Moderately Telolecithal

3. telolecithal

Term
Isolecithal
Definition
yolk evenly distributed in egg (microlecithal)
Term
Moderately Telolecithal
Definition
more concentrated in one region of the egg (mesolecithal)
Term
Telolecithal
Definition
densely concentrated in one region of the egg (macrolecithal)
Term
Holoblasric
Definition
entire embryo undergoes cleavage
Term
Holoblastic equal
Definition
found in isolecithal eggs-all blastomeres divide evenly throughout so all are approximately the same size.
Term
Holoblastic unequal
Definition
found in Moderately telolecithal eggs - blastomeres in vegetal pole divide more slowly than in animal, so blastomeres in the vegetal pole are larger and fewer in number (macromeres) - blastomeres in animal pole are smaller and more numerous (micromeres)
Term
Meroblastic 
Definition
cleavage is confined to one region of embryo away from yolk 
Term
grastulation
Definition
embryo now begins to change size and shape. dividing blastomeres now push inward forming a hole on the surface of the embryo (the blastopore). continued division inward of these blastomeres produces a tube inside the embryo which is the beginning of the digestive system and is called the archenteron.
Term
germ layers
Definition
three kinds of layers
Term
Ectoderm
Definition
the outer layer forms skin and also the nervous system
Term
Mesoderm
Definition
the middle layer which produces muscles and the internal skeleton on many animals
Term
Endoderm
Definition
the inner layer forms the linings of all tubes in the body ie: stomach lining
Term
neurulation
Definition
formation of the central nervous system
Term
neural ridges or neural folds
Definition
the system forms from the build up ectodermal cell along the long axis of the body to form two ridges
Term
neural groove
Definition
lying between the ridges 
Term
neural canal
Definition
the neural ridges continue to enlarge until they meet over the center of the groove. it is a hollow tube that eventually fills in with nerve cells becoming the spinal cord.
Term
notochord
Definition
fibrous rod of mesodermal tissue directily beneath and parallel to the neural tube = spine
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